Qhov Tseem Ceeb - TLC vs HPTLC
TLC thiab HPTLC yog ob txoj kev siv tshuaj siv los cais cov khoom tsis sib xws hauv kev sib tov. Qhov sib txawv tseem ceeb ntawm TLC thiab HPTLC yog tias cov khoom nqus dej hauv TLC phaj muaj cov khoom loj thaum HPTLC daim hlau muaj cov khoom me me ntawm cov khoom adsorbent.
TLC yog nyias txheej chromatography. HPTLC yog cov txheej txheem nyias nyias chromatography, uas yog cov txheej txheem siab heev thaum piv rau TLC txoj kev.
TLC yog dab tsi?
TLC lossis nyias txheej chromatography yog cov txheej txheem tshuaj yooj yim siv los cais cov khoom tsis sib xws hauv kev sib xyaw. Qhov kev sim no yog ua los ntawm cov ntawv iav, yas lossis ntawv. Daim ntawv no yog coated nrog nyias txheej ntawm cellulose los yog silica gel (ib qho khoom adsorbent). Cov txheej nyias no yog hu ua lub sijhawm nyob ruaj khov ntawm TLC. Daim ntawv hu ua TLC phaj.
Ua ntej qhov kev sim, cov kab hauv qab thiab cov kab saum toj kawg nkaus tau cim nrog tus cwj mem ntawm TLC phaj. Tom qab ntawd cov qauv yog pom raws kab no siv lub raj capillary. Tom qab ntawd, TLC phaj yog muab tso rau vertically nyob rau hauv ib tug mobile theem. Lub sijhawm txawb yog cov kuab tshuaj (lossis sib xyaw ntawm cov kuab tshuaj) muab tso rau hauv lub thawv. Thaum muab TLC phaj nyob rau hauv lub sij hawm mobile no, ib tug yuav tsum khaws cov hauv paus ntsiab lus ntawm cov npoo ntawm cov kuab tshuaj. Txwv tsis pub, cov qauv yuav yaj hauv cov kuab tshuaj.
TLC phaj tau tso cai nyob hauv theem mobile rau qee lub sijhawm. Tom qab ntawd, nrog rau lub sijhawm, lub sijhawm txawb nce siab ntawm lub phaj vim kev ua haujlwm capillary. Yog hais tias tus qauv yog soluble nyob rau hauv lub mobile theem, nws yuav nce upward nrog rau lub mobile theem.
Lub ntsiab lus "zoo li yaj zoo li" yog tom qab txoj kev TLC no. Qhov no txhais tau tias polar compounds yaj nyob rau hauv polar solvents whereas nonpolar compounds dissolve nyob rau hauv nonpolar solvents. Cov theem nyob ruaj khov feem ntau yog silica gel lossis cellulose, uas yog cov polar compound. Cov theem txawb siv yog cov kuab tshuaj nonpolar xws li hexane. Yog li ntawd, yog tias tus qauv muaj cov khoom tsis muaj polar, cov khoom no yuav txav mus rau sab saud nrog rau theem mobile. Polar Cheebtsam yuav lo rau lub phaj. Ntawm cov khoom txav mus, cov tebchaw nruab nrab uas tsis muaj polar yuav txav mus qeeb thaum cov tshuaj tsis muaj polar ntau yuav tsiv mus sai. Thaum cov kuab tshuaj pem hauv ntej tau mus txog kab saum toj kawg nkaus ntawm TLC phaj, lub phaj raug muab tshem tawm thiab cia kom qhuav.
Daim duab 01: TLC Technique
Yog tias cov khoom hauv cov qauv yog cov xim sib xyaw, yuav tsum muaj cov xim muaj xim ntawm lub phaj ntawm ntau qhov chaw. Tab sis yog tias cov khoom tsis muaj xim, yuav tsum tau siv lwm cov txheej txheem; Piv txwv li, tshuaj tsuag ninhydrin kom kuaj tau ammonia lossis amines (primary amines thiab secondary amines).
HPTLC yog dab tsi?
HPTLC lossis kev ua haujlwm siab txheej nyias chromatography yog daim ntawv siab tshaj ntawm txheej nyias nyias chromatography (TLC). Qhov kev daws teeb meem thiab qhov tseeb ntawm HPTLC yog qhov siab heev. Feem ntau cov kauj ruam ntawm cov txheej txheem no tuaj yeem siv tau.
Cov phaj siv hauv HPTLC muaj cov khoom siv silica gel nrog qhov me me thiab cov ntim ntim ntawm cov gel ntawm lub phaj siab. Qhov saum npoo ntawm lub phaj yog du thiab muab kev sib cais zoo. Kev tsom xam sai dua li cov txheej txheem TLC. Lub rhiab heev ntawm txoj kev no kuj yog siab. Hauv HPTLC, ntau cov qauv sib txawv tuaj yeem sib cais hauv tib lub phaj yam tsis muaj cov qauv npaj ua ntej.
Qhov txawv ntawm TLC thiab HPTLC yog dab tsi?
TLC vs HPLC |
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| TLC yog nyias txheej chromatography. | HPTLC yog kev ua haujlwm siab nyias txheej chromatography. |
| Technique | |
| Nws yog cov txheej txheem tshuaj yooj yim siv los cais cov khoom tsis sib xws hauv qhov sib tov. | Nws yog daim ntawv qib siab ntawm nyias txheej chromatography (TLC). |
| TLC phaj tuaj yeem ua los ntawm ntawv, yas lossis iav uas yog siv nrog cov khoom siv adsorbent uas muaj cov khoom loj. | HPTLC daim hlau muaj cov khoom me me ntawm cov khoom adsorbent. |
| Kev daws teeb meem | |
| TLC txoj kev muab kev daws teeb meem tsis zoo thaum tus qauv muaj cov khoom sib xws. | HPTLC txoj kev muab qhov kev daws teeb meem siab. |
| TLC txheej txheem yog cov txheej txheem siv sijhawm. | HPTLC muab cov txiaj ntsig ntawm qhov nrawm dua li TLC txoj kev. |
Summary – TLC vs HPLC
HPTLC yog daim ntawv qib siab ntawm TLC. Qhov sib txawv tseem ceeb ntawm TLC thiab HPTLC yog tias TLC phaj tuaj yeem ua los ntawm cov ntawv, yas lossis iav uas yog siv nrog cov khoom siv adsorbent uas muaj cov khoom loj thaum HPTLC daim hlau muaj cov khoom me me ntawm cov khoom adsorbent.
