Qhov Tseem Ceeb Qhov txawv - Random Mutagenesis vs Site Directed Mutagenesis
Mutagenesis yog txheej txheem uas hloov pauv tau lub hom phiaj qhia rau cov hlwb lossis cov noob uas yuav ua rau cov noob hloov pauv lossis cov kab mob. Mutagenesis feem ntau yog ua los qhia cov yam ntxwv zoo rau cov kab mob. Tam sim no mutagenesis kuj tau siv hauv kev kho cov noob los hloov cov noob thiab rau cov hom phiaj tshuaj. Kev hloov pauv tuaj yeem qhia tau ob txoj hauv kev loj; Random mutagenesis thiab Site directed mutagenesis. Random mutagenesis yog txheej txheem ntawm kev qhia txog kev hloov pauv random. Tom qab ntawd xaiv cov kab mob uas hloov pauv siv txoj kev xaiv. Cov txheej txheem yog random nkaus. Site Directed mutagenesis yog cov txheej txheem uas kev hloov pauv tau qhia hauv qhov chaw tshwj xeeb rau cov chaw tshwj xeeb hauv DNA lossis rau cov nucleotides tshwj xeeb. Qhov tshwj xeeb ntawm qhov chaw qhia kev hloov pauv hauv siab heev. Qhov sib txawv tseem ceeb ntawm random thiab qhov chaw qhia mutagenesis yog lub zam uas qhov kev hloov pauv tau qhia. Random mutagenesis qhia txog kev hloov pauv hauv kev sib txawv, qhov chaw qhia kev hloov pauv tshwj xeeb yog tsom rau cov chaw xaiv ntawm cov noob.
Random Mutagenesis yog dab tsi?
Random mutagenesis yog hais txog cov txheej txheem ntawm kev qhia txog kev hloov pauv mus rau cov kab mob hauv kev sib txawv thiab yog li tsis yog tshwj xeeb. Random mutagenesis suav nrog kev nthuav tawm cov kab mob mus rau hauv mutagen rau lub sijhawm thiab xaiv cov mutant ntau yam. Cov mutagens tuaj yeem yog lub cev mutagens zoo li UV hluav taws xob lossis tshuaj mutagens xws li alkylating agents. Txoj kev no yog qhov zoo tshaj plaws los txhawb kev hloov pauv hauv cov kab mob, nroj tsuag, thiab tsiaj txhu.
Cov txheej txheem kev txhim kho microbial hauv kev tsim tshuaj tua kab mob yog ua raws li kev hloov pauv hloov pauv. Cov kab mob uas tsim cov tshuaj tua kab mob yog raug rau cov mutagens sib txawv thiab tom qab ntawd kuaj cov tshuaj tua kab mob ntawm cov kab mob. Yog li ntawd, cov kab mob uas tau tsim kev tawm tsam dhau qhov kev tawm tsam thawj zaug tuaj yeem raug txiav tawm.
Nyob rau hauv cov ntaub so ntswg kab lis kev cai, random mutagenesis yog siv los muab cov cim sib txawv rau hauv cov nroj tsuag. Cov nroj tsuag raug rau mutagens nyob rau hauv lub callus forming theem. Hauv kev tshawb fawb tsiaj, random mutagenesis tsis tshua muaj siv, txawm hais tias cov tshuaj alkylating tau siv los ua kom muaj kev hloov pauv ntawm kev hloov pauv ntawm random mutagenesis.
Thaum nthuav tawm cov kab mob rau mutagens, kev saib xyuas yuav tsum tau saib xyuas txog koob tshuaj mutagen thiab lub sijhawm raug rau cov mutagens. Cov koob tshuaj ntau dhau thiab lub sijhawm raug cuam tshuam tuaj yeem ua rau lub cev puas tsuaj. Yog li ntawd, random mutagenesis yuav tsum tau ua raws li kev tswj xyuas.
Qhov Chaw Qhia Qhia Mutagenesis yog dab tsi?
Qhov chaw qhia mutagenesis yog txheej txheem tshwj xeeb ntawm kev hloov pauv, qhov twg cov analogs tau siv los tsim cov kev hloov pauv. Cov analogues hauv qab no muaj peev xwm ua rau muaj kev hloov pauv Adenine-Thymine rau Guanine-Cytosine. Hauv qhov no, qhov kev hloov pauv tau tshwm sim thiab nws yog lub npe hu ua qhov chaw qhia mutagenesis thiab yog li ntawd muaj qhov tshwj xeeb.
Daim duab 01: Qhov Chaw Qhia Qhia Mutagenesis
Nyob rau hauv cov txheej txheem biology thaum ntxov, lub vev xaib tau qhia mutagenesis siv cov tshuaj analogs tau ua tiav siv aminopurine rau AT - GC kev hloov pauv thiab nitrosoguanidine rau GC rau AT kev hloov pauv. Nrog rau kev qhia txog Polymerase Chain Reaction (PCR) thiab lub tswv yim ntawm primers, tam sim no, qhov chaw qhia mutagenesis yog induced los ntawm mutagenic oligonucleotides. Txoj kev no tso cai rau kev nkag ntawm indels, thiab taw qhia kev hloov pauv rau cov noob los yog kab mob. Tom qab ua tiav cov txheej txheem mutagenesis, cov mutants raug xaiv los ntawm kev siv cov neeg sau xov xwm tshwj xeeb lossis cov cim.
Dab tsi yog qhov zoo sib xws ntawm Random Mutagenesis thiab Site Directed Mutagenesis?
- Ob qho tib si Random Mutagenesis thiab Site Directed Mutagenesis cov txheej txheem yog cov txheej txheem tsim kom muaj kev hloov pauv mus rau ib qho kab mob tshwj xeeb.
- Ob qho tib si Random Mutagenesis thiab Site Directed Mutagenesis cov tswv yim yog siv los tsim cov khoom muaj txiaj ntsig zoo uas muaj cov yam ntxwv xws li kev tiv thaiv kab mob thiab hauv kev kho noob.
- Ob qho tib si Random Mutagenesis thiab Site Directed Mutagenesis cov tswv yim ua rau muaj kev hloov pauv hauv DNA thiab hloov DNA.
- Ob qho tib si Random Mutagenesis thiab Site Directed Mutagenesis hom yog induced mas los ntawm lub cev thiab tshuaj.
Dab tsi yog qhov txawv ntawm Random Mutagenesis thiab Site Directed Mutagenesis?
Random Mutagenesis vs Site Directed Mutagenesis |
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| Random mutagenesis yog txheej txheem ntawm kev qhia txog kev hloov pauv hloov pauv thiab tom qab ntawd xaiv cov kab mob uas tau hloov pauv siv txoj kev xaiv. Cov txheej txheem yog random nkaus. | Chaw Qhia Qhia mutagenesis yog cov txheej txheem uas kev hloov pauv tau qhia hauv qhov chaw tshwj xeeb rau cov chaw tshwj xeeb hauv DNA lossis rau cov nucleotides tshwj xeeb. |
Summary – Random Mutagenesis vs Site Directed Mutagenesis
Kev hloov pauv yog kev hloov pauv hauv DNA kab ke ntawm lub cev. Cov no yog vim tsis paub qhov tshwm sim ntawm mutagens. Nyob rau hauv mutagenesis, ntau yam ntawm cov kab mob, cov nroj tsuag thiab tsiaj cell kab lis kev cai raug mus rau mutagens thiab cov mutant ntau yam yog xaiv tom qab ua tiav ntawm cov noob hloov. Nyob ntawm seb qhov kev hloov pauv tau coj mus li cas, lawv tuaj yeem yog Random mutations lossis site-directed mutations. Random mutagenesis yog cov txheej txheem qhia txog kev hloov pauv tsis tu ncua thaum Site Directed mutagenesis yog cov txheej txheem uas kev hloov pauv tau qhia hauv qhov chaw tshwj xeeb rau qhov chaw tshwj xeeb hauv DNA lossis rau cov nucleotides tshwj xeeb. Qhov no yog qhov txawv ntawm Random mutagenesis thiab Site Directed mutagenesis.
