Qhov Tseem Ceeb Qhov Sib Txawv - Khoom Media vs Semi Solid Media
Cov kab lis kev cai nruab nrab tuaj yeem txhais tau tias yog cov khoom tsim los yog kua uas muaj cov as-ham thiab lwm yam tsim nyog rau kev loj hlob ntawm cov kab mob thiab cov hlwb. Cov kab lis kev cai nruab nrab yog siv los loj hlob cov kab mob nyob rau hauv lub chaw kuaj mob rau ntau lub hom phiaj xws li kev tshawb fawb, kev txheeb xyuas, kev faib tawm, kev tsim tshuaj, recombinant DNA technology, enzyme extraction thiab lwm yam. Muaj ntau hom kab lis kev cai xov xwm. Raws li qhov sib xws, kab lis kev cai xov xwm yog peb hom; khoom media, semi solid media thiab kua media. Cov khoom siv xov xwm tau npaj siv tus neeg sawv cev inert solidification (agar) ntawm qhov concentration ntawm 1.5 mus rau 2.0%. Semi khoom xov xwm yog npaj siv ib tug solidifying tus neeg sawv cev (agar) ntawm 0.2 mus rau 0.5%. Qhov tseem ceeb sib txawv ntawm cov khoom siv xov xwm thiab cov khoom siv semi-tshuaj yog tias cov khoom siv xov xwm muaj cov ntsiab lus siab ntawm agar thiab yog siv rau kev txheeb xyuas thiab ua tus cwj pwm ntawm cov kab mob colony morphologies ntawm cov kab mob thaum cov khoom siv semi-tshuaj muaj cov ntsiab lus tsawg ntawm agar thiab yog siv los txiav txim siab. ntawm motility ntawm cov kab mob.
Dab tsi yog Media?
Cov xov xwm khoom yog ib hom kev loj hlob lossis kab lis kev cai xov xwm uas siv rau cov kab mob loj hlob lossis cov hlwb hauv cov chaw kuaj mob. Qhov nruab nrab yog npaj los ntawm kev sib xyaw cov khoom tsim nyog thiab cov ntaub ntawv ntawm cov concentration kom raug. Lwm yam tshaj li cov as-ham, tus neeg sawv cev solidification yog siv thaum lub sij hawm npaj cov khoom thiab Semi Solid Media. Tus neeg sawv cev solidification siv hauv kev npaj tawm yog agar. Agar yog ib qho inert tshuaj muab rho tawm los ntawm hiav txwv algae. Nws tsis qhia qhov khoom noj muaj txiaj ntsig.
Daim duab 01: Khoom Media
Cov xov xwm muaj zog muaj cov ntsiab lus siab ntawm agar. Agar yog ntxiv ntawm 1.5 mus rau 2.0% concentration. Agar solidifies qhov nruab nrab hauv qab 40 0 C. Thaum nruab nrab solidifies, nws tso cai rau ib tug khoom nto rau streak thiab loj hlob microorganisms. Khoom siv xov xwm yog siv los txheeb xyuas cov kab mob. Thiab lawv kuj tau siv los kawm txog cov yam ntxwv ntawm cov kab mob sib txawv thiab kev kawm ntawm cov kab mob colony.
Semi Solid Media yog dab tsi?
Ntau txoj kev siv los soj ntsuam thiab kuaj xyuas cov kab mob motility. Ntawm lawv dai poob txoj kev yog ib txoj kev zoo li no. Txawm li cas los xij, nws muaj ob peb qhov tsis zoo xws li qhov tsis txaus ntseeg ntawm txoj kev, qhov tsis paub tseeb ntawm cov txiaj ntsig, qhov nyuaj ntawm kev txheeb xyuas qhov kev txav mus los thaum tsuas yog ob peb lub hlwb yog motile, xav tau kev ua haujlwm lossis cov kab lis kev cai tshiab thiab lwm yam. Yog li ntawd, cov kws tshawb fawb tau tsim cov khoom siv semi-tiav rau saum lub hom phiaj. Semi khoom xov xwm yog microbial kab lis kev cai xov xwm uas tau npaj kom muaj tsawg dua ntawm agar (solidifying tus neeg sawv cev ntawm 0.2 mus rau 0.5%) los soj ntsuam cov kab mob motility. Semi khoom nruab nrab yog thawj zaug qhia los ntawm Hiss hauv xyoo 1982 rau lub hom phiaj ntawm kev paub qhov txawv ntawm tus kab mob typhoid thiab cov kab mob hauv plab.
Daim duab 02: Stab Tube
Cov txiaj ntsig ntawm cov xov xwm semi khoom yog macroscopic. Thaum cov kab mob motile yog inoculated rau stab kab lis kev cai uas tau npaj siv Semi Solid Media, ib qho chaw diffuse ntawm kev loj hlob raws kab inoculation ntawm tus stab yuav pom meej meej. Nws tshem tawm qhov kev pom ntawm kev txav mus los yog tias tsuas yog ob peb lub motile.
Dab tsi yog qhov zoo sib xws ntawm Cov Media thiab Semi Solid Media?
- Solid thiab Semi Solid Media yog hom kab lis kev cai tshaj tawm raws li qhov sib xws.
- Ob leeg siv los ua kab mob.
- Ob lub xov xwm muaj cov khoom noj.
- Ob lub xov xwm muaj tus neeg sawv cev khov kho.
- Ob qho xov xwm tseem ceeb hauv microbiology.
Dab tsi yog qhov txawv ntawm Khoom Media thiab Semi Solid Media?
Solid Media vs Semi Solid Media |
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| Solid Media yog hom kab lis kev cai uas muaj agar ntawm 1.5 txog 2.0% concentration. | Semi Solid Media yog hom kab lis kev cai uas muaj agar ntawm 0.5% concentration. |
| Solid Media yog qhov muaj txiaj ntsig rau kev cais thiab suav cov kab mob los yog txiav txim siab cov yam ntxwv ntawm pawg. | Semi Solid Media yog siv rau kev txiav txim siab ntawm cov kab mob motility. |
| Khoom Media yog ruaj khov thiab muaj lub ntsej muag khov kho vim agar. | Semi Solid Media muaj cov mos mos zoo li jelly. |
Summary – Solid Media vs Semi Solid Media
kab lis kev cai nruab nrab muaj cov khoom noj sib txawv thiab lwm yam khoom siv xws li dej, qhov chaw ntawm carbon thiab lub zog, qhov chaw ntawm nitrogen, minerals thiab ntau yam kev loj hlob thiab lwm yam rau kev loj hlob ntawm cov kab mob thiab cov hlwb. Khoom thiab Semi Solid Media yog ob hom kev tshaj tawm uas tau muab cais raws li qhov sib xws ntawm qhov nruab nrab. Khoom nruab nrab muaj 1.5 mus rau 2.0% solidification tus neeg sawv cev thaum ib nrab khoom nruab nrab muaj 0.2 mus rau 0.5% solidifying agent. Thaum poured rau hauv daim hlau, khoom nruab nrab solidifies thiab muab ib tug khoom nto kom loj hlob microorganisms. Semi-khoom nruab nrab yog mos, thiab nws tsis solidify tag nrho raws li cov khoom siv. Li no, Semi Solid Media tso cai rau cov kab mob motile txav thiab loj hlob nyob rau hauv nruab nrab, tsis zoo li cov khoom siv xov xwm. Cov khoom nruab nrab yog siv los txheeb xyuas thiab ua tus yam ntxwv ntawm cov kab mob thiab lwm yam kab mob thaum cov khoom nruab nrab yog siv rau kev txiav txim siab ntawm cov kab mob motility. Qhov no yog qhov sib txawv ntawm cov khoom siv xov xwm thiab cov xov xwm semi solid.
Download PDF Solid Media vs Semi Solid Media
Koj tuaj yeem rub tawm PDF version ntawm kab lus no thiab siv nws rau offline hom phiaj raws li daim ntawv ceeb toom. Thov rub tawm PDF version ntawm no Qhov txawv Ntawm Khoom Media thiab Semi Solid Media
