Qhov Tseem Ceeb Qhov txawv - Hauv Situ Hybridization vs Immunohistochemistry
Kev kuaj mob qog noj ntshav thiab kis kab mob yog qhov nrov nrov uas cov txheej txheem tshiab proteomics thiab genomics raws li kev siv rau lub hom phiaj ntawm kev txheeb xyuas cov qog lossis cov kab mob sib kis, nws qhov kev loj hlob thiab qhov chaw ntawm kev loj hlob ntawm tes thiab tshuaj xyuas cov noob caj noob ces feem ntau kis tau thiab Cov kab mob tsis sib kis. Qhov no yuav ua rau kev ua tshuaj raug cai thiab tsim thiab tsim kho kev kho mob rau cov kab mob. Nyob rau hauv situ hybridization (ISH) thiab Immunohistochemistry (IHC) yog ob txoj kev siv dav hauv kev mob qog noj ntshav thiab qhov sib txawv tseem ceeb ntawm qhov sib txawv ntawm situ hybridization thiab immunochemistry lies nyob rau hauv cov molecules uas siv nyob rau hauv cov txheej txheem tsom xam. Hauv ISH, cov tshuaj ntsuam xyuas nucleic acid tau siv rau hauv kev tshuaj ntsuam thaum, hauv IHC, monoclonal thiab polyclonal antibodies yog siv rau kev txiav txim siab.
Dab tsi yog Hauv Situ Hybridization (ISH)?
In situ hybridization yog cov txheej txheem nucleic acid hybridization uas tau ua ncaj qha rau ntawm ib feem lossis ntu ntawm cov ntaub so ntswg, hauv tag nrho cov ntaub so ntswg lossis hauv hlwb. Cov txheej txheem yog nyob ntawm txoj kev xav ntawm Watson Crick ua ke hauv paus ua ke, ua rau DNA-DNA hybrids lossis DNA-RNA hybrids uas tuaj yeem ntes cov noob hloov pauv lossis txheeb xyuas cov noob xav tau ntawm kev txaus siab. Tib txoj hlua DNA ib ntus, ob txoj hlua DNA ib ntus, ib leeg RNA ib ntus lossis cov khoom sib txuas oligonucleotide yog siv los ua kev sojntsuam thaum cov txheej txheem hybridization, thiab cov kev sojntsuam no tau sau nrog cov xov tooj cua phosphorus ntawm nws 5 'kawg rau kev txheeb xyuas cov txheej txheem fluorography los yog autoiradescent.. Muaj ntau hom ISH cov txheej txheem muaj raws li hom kev sojntsuam siv thiab hom kev pom kev ua raws li.
Daim duab 01: fluorescent Hauv Situ Hybridization
Muaj ntau daim ntawv thov ntawm ISH, feem ntau hauv kev kuaj mob molecular ntawm cov kab mob sib kis txhawm rau txheeb xyuas qhov muaj cov kab mob thiab kom paub meej cov kab mob ntawm kev kuaj mob molecular. Nws kuj yog siv nyob rau hauv kev loj hlob biology, karyotyping thiab phylogenetic tsom xam thiab lub cev daim ntawv qhia ntawm chromosomes.
Immunohistochemistry (IHC) yog dab tsi?
Nyob rau hauv cov txheej txheem ntawm IHC, lub ntsiab molecule soj ntsuam yog cov antigen. Thaum lub sij hawm IHC, monoclonal thiab polyclonal antibodies yog siv los txiav txim seb muaj cov antigens thaum muaj kab mob los yog malignant cell proliferation. Cov txheej txheem yog raws li antigen-antibody khi, thiab cov ntawv enzyme yog siv rau cov txheej txheem no; Ib daim ntawv thov no yog ELISA (Enzyme linked immunosorbent assay). Cov cim tseem tuaj yeem yog cov tshuaj tiv thaiv kab mob fluorescent lossis xov tooj cua sau cov tshuaj tiv thaiv.
Daim duab 02: Immunohistochemistry
IHC yog siv dav rau kev kuaj mob qog noj ntshav. Cov txheej txheem kuaj mob tsom cov antigens tam sim no ntawm cov qog hlwb txhawm rau txheeb xyuas thiab txheeb xyuas cov qog. Cov txheej txheem tib yam yog suav nrog txhawm rau kuaj cov kab mob sib kis. Monoclonal thiab Polyclonal antibodies kuj tseem siv los txheeb xyuas cov khoom sib txawv ntawm cov noob caj noob ces los ntawm kev ua kom cov tshuaj tiv thaiv kab mob-antigen binding cov tshuaj tiv thaiv ntawm cov protein uas xav tau thiab cov khoom siv tshuaj tua kab mob.
Dab tsi zoo sib xws ntawm Hauv Situ Hybridization thiab Immunohistochemistry?
- ISH thiab IHC yog cov tshuaj tiv thaiv tshwj xeeb.
- Ob txoj kev yog qhov tseeb heev.
- Ob txoj kev siv tau los kuaj mob qog noj ntshav thiab kis kab mob.
- Cov tswv yim no tau ua nyob rau hauv qhov chaw tsis muaj menyuam hauv vitro.
- Ob leeg yog cov txheej txheem nrawm uas muab cov txiaj ntsig rov tsim dua.
- ISH thiab IHC siv txoj hauv kev tshawb pom xws li xov tooj cua sau npe, thiab cov txheej txheem fluorescence.
Dab tsi yog qhov txawv ntawm Hauv Situ Hybridization thiab Immunohistochemistry?
YIn Situ Hybridization vs Immunohistochemistry |
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| ISH yog cov txheej txheem nucleic acid hybridization uas tau ua ncaj qha rau ntawm ib feem lossis ntu ntawm cov ntaub so ntswg lossis tag nrho cov ntaub so ntswg. | IHC yog cov txheej txheem uas siv cov tshuaj tiv thaiv kab mob monoclonal thiab polyclonal los txiav txim siab muaj cov tshuaj tiv thaiv, uas yog cov cim tshwj xeeb ntawm cov protein tso rau ntawm lub xov tooj ntawm tes. |
| Yam Bio Molecules Analysed | |
| ISH tshuaj ntsuam nucleic acids. | IHC tshuaj xyuas cov proteins-antigens. |
| Basis ntawm Biochemical Reaction | |
| Complementary base pairing ntawm DNA-DNA lossis DNA-RNA tshwm sim hauv cov txheej txheem no. | Antigen-antibody kev sib cuam tshuam yog koom nrog hauv immunohistochemistry. |
| Txoj Kev Tshawb Nrhiav Enzyme-Txuas Kev Tshawb Nrhiav | |
| Txoj kev tshawb nrhiav cov enzyme tsis tuaj yeem siv hauv ISH. | Txoj kev tshawb nrhiav qhov txuas Enzyme tuaj yeem siv hauv IHC. |
Summary – In Situ Hybridization vs Immunohistochemistry
Kev kuaj mob Molecular yog cov txheej txheem ceev thiab paub tseeb uas tuaj yeem siv los txheeb xyuas cov kab mob tsis sib kis xws li mob qog noj ntshav lossis kab mob sib kis xws li HIV lossis Tuberculosis raws li cov cim molecular muaj nyob rau ntawm cov hlwb uas ua rau pom tus kab mob.. Molecular markers tuaj yeem muaj nyob rau hauv daim ntawv ntawm cov proteins uas tau hais los yog nyob rau theem caj ces raws li cov txheej txheem tshiab tshiab tau qhia los ua kom muaj txiaj ntsig thiab tsis muaj zog, txawm hais tias muaj tus nqi siab koom nrog cov tswv yim no. Yog li ISH nyob ntawm DNA-DNA lossis DNA-RNA hybrid tsim, thiab IHC nyob ntawm cov tshuaj tiv thaiv tshwj xeeb ntawm cov tshuaj tiv thaiv thiab antigen. Qhov no yog qhov txawv ntawm qhov chaw hybridization.
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Koj tuaj yeem rub tawm PDF version ntawm kab lus no thiab siv nws rau offline hom phiaj raws li daim ntawv ceeb toom. Thov download PDF version ntawm no Qhov txawv Ntawm Hauv Situ Hybridization thiab Immunohistochemistry.
