Key Difference – AFLP vs RFLP
DNA cov kev tshawb fawb muaj qhov tseem ceeb hauv kev nkag siab thiab txiav txim siab txog kev sib raug zoo ntawm phylogenetic, kuaj cov kab mob caj ces thiab daim phiaj kab mob genomes. Ntau cov txheej txheem cuam tshuam nrog kev txheeb xyuas DNA kuj tseem siv rau kev txheeb xyuas cov noob tshwj xeeb lossis cov txheej txheem DNA hauv ib lub pas dej ntawm DNA tsis paub. Lawv hu ua molecular markers. Amplified Fragment Length Polymorphism (AFLP) thiab Restriction Fragment Length Polymorphism (RFLP) yog ob qho xws li cov cim molecular (txoj kev) tsim nyob rau hauv molecular biology txhawm rau txheeb xyuas cov kev hloov pauv ntawm cov kab mob. Ob txoj kev yog qhov tseem ceeb sib npaug thiab muaj qhov zoo thiab qhov tsis zoo. Qhov sib txawv tseem ceeb ntawm AFLP thiab RFLP yog tias AFLP koom nrog kev xaiv PCR kev nthuav dav ntawm cov zom zom DNA thaum RFLP tsis koom nrog kev xaiv PCR nthuav dav ntawm DNA tawg.
AFLP yog dab tsi?
AFLP (Amplified Fragment Length Polymorphism) yog ib qho cuab yeej tseem ceeb hauv molecular biology thiab tau siv dav hauv kev tshuaj ntsuam genetic variation analysis. AFLP yog raws li qhov tshwj xeeb PCR amplification ntawm fragmented genomic DNA thiab nrhiav pom ntawm polymorphism los ntawm autoradiographs ntawm gel electrophoresis. AFLP tau nthuav dav rau kev txheeb xyuas qhov sib txawv ntawm cov noob caj noob ces hauv cov kab mob lossis cov tsiaj sib ze ntawm ntau lub tebchaws suav nrog cov nroj tsuag, tsiaj txhu, kab mob thiab cov kab mob fungi. AFLP tuaj yeem nqa nrog me me ntawm cov qauv DNA tsis paub. Nws tsis tas yuav muaj kev paub ua ntej ua ntej thiab tsim kev sojntsuam.
Steps of AFLP
- Kev cais tawm ntawm DNA
- Kev zom ntawm DNA nrog txwv endonucleases
- Ligation ntawm txwv DNA tawg tawg nrog adaptors
- Xaiv qhov kev nthuav dav ntawm cov khoom tawg nrog cov chaw tshwj xeeb txwv
- Sib cais ntawm cov khoom PCR los ntawm gel electrophoresis
- Kev pom ntawm gel matrix los ntawm autoradiograph
AFLP yog ib txoj hauv kev yooj yim dua thiab rov tsim dua tshiab uas tuaj yeem siv rau hauv DNA profileing ntawm ntau cov se nrog rau cov kab mob, cov kab mob, cov nroj tsuag thiab tsiaj yam tsis muaj kev paub txog DNA ua ntej. Nws pab txhawm rau txheeb xyuas qhov sib txawv me ntsis ntawm cov tib neeg hauv cov pej xeem vim nws qhov xwm txheej rhiab heev. AFLP tseem yog ib qho tseem ceeb hauv genome mapping, kev tshawb fawb forensic, kuaj niam txiv, genotyping, thiab lwm yam.
Daim duab 01: AFLP
RFLP yog dab tsi?
Txoj Kev Txwv Fragment Length Polymorphisms (RFLPs) yog cov txheej txheem uas siv los txheeb xyuas cov kev hloov pauv caj ces hauv homologous DNA sequences. Nws yog thawj txoj kev tsim rau DNA profileing. Cov kab mob muaj cov cim DNA ntiv tes lossis DNA profile. RFLP yog ib qho cuab yeej tseem ceeb rau kev txheeb xyuas qhov sib txawv ntawm DNA profiles ntawm cov kab mob intraspecific los yog ze ze vim hais tias homologous sequences muaj qhov sib txawv txwv qhov chaw (qhov chaw) uas yog tshwj xeeb rau ib yam kab mob. Thaum homologous DNA tau zom nrog cov kev txwv tshwj xeeb endonucleases, nws yuav ua rau cov DNA sib txawv uas txawv rau txhua tus neeg. Yog li ntawd, lub hauv paus tseem ceeb ntawm txoj kev no yog kev tshawb nrhiav kev hloov pauv caj ces ntawm cov kab mob los ntawm kev txwv homologous DNA nrog cov kev txwv tshwj xeeb enzymes thiab kev tsom xam ntawm qhov ntev ntawm polymorphism ntawm gel electrophoresis thiab blotting. Blotting qauv yog tshwj xeeb rau txhua yam kab mob thiab ua tus yam ntxwv ntawm genotypes.
Steps of RFLP
- Kev cais cov DNA txaus los ntawm cov qauv
- Kev tawg ntawm DNA cov qauv nrog kev txwv tshwj xeeb endonucleases rau hauv ntu luv
- Kev sib cais ntawm qhov tshwm sim ntawm qhov sib txawv ntawm qhov ntev los ntawm agarose gel electrophoresis.
- Hloov cov gel profile rau hauv daim nyias nyias los ntawm Southern blotting
- Hybridization ntawm daim nyias nyias nrog cov ntawv lo sojntsuam thiab kev tshuaj xyuas ntawm cov ntu ntev polymorphism hauv txhua qhov profile
RFLP yog cov txheej txheem tseem ceeb hauv kev txheeb xyuas cov kab mob qub txeeg qub teg thiab nrhiav kev pheej hmoo ntawm tus kab mob tshwm sim ntawm cov neeg hauv tsev neeg. RFLP kuj nquag siv nyob rau hauv genome mapping, txheeb xyuas cov neeg ua txhaum cai hauv forensics, kuaj niam txiv, thiab lwm yam. RFLP muaj ntau yam kev txwv thiab. RFLP xav tau kev paub ua ntej ntawm cov ntaub ntawv ua ntu zus los tsim kev sojntsuam rau hybridization. Nws tseem yuav tsum tau cais cov DNA txaus los ntawm cov qauv los txheeb xyuas, uas nyuaj rau kev tshawb fawb forensic.
Daim duab 01: RRFLP mapping
Qhov txawv ntawm AFLP thiab RFLP yog dab tsi?
ALFP vs RFLP |
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| AFLP suav nrog kev xaiv PCR nthuav dav ntawm DNA zom. | RFLP tsis koom nrog PCR tshwj tsis yog nws yog PCR-RFLP. |
| Kev Paub | |
| kev paub ua ntej tsis tas yuav tsum tau. | Kev paub ua ntej yuav tsum tau tsim los tsim RFLP probes. |
| Qhov no ntseeg tau ntau dua. | Qhov no tsis tshua ntseeg siab piv rau AFLP. |
| Kev Ua Haujlwm Hauv Kev Tshawb Nrhiav Polymorphism | |
| Qhov no muaj qhov ua tau zoo dua hauv kev kuaj pom polymorphism dua li RFLP. | Qhov no ua haujlwm tsawg dua piv rau AFLP. |
| Tus nqi | |
| Qhov no kim me ntsis piv rau RFLP. | Qhov no tsis kim dua piv rau AFLP. |
| Daim ntawv thov | |
| AFLPs tau raug siv rau genome mapping, DNA ntiv tes, kev tshawb fawb txog kev sib txawv ntawm caj ces, kuaj txiv neej thiab forensics | RFLP kev tshuaj xyuas yog ib qho cuab yeej tseem ceeb hauv genome mapping, thaj chaw ntawm cov noob rau cov kab mob caj ces, kev txiav txim siab ntawm kev pheej hmoo rau tus kab mob, thiab kuaj niam txiv. |
Summary – AFLP vs RFLP
AFLP thiab RFLP yog ob txoj kev siv los ua cov cim caj ces rau kev ntsuam xyuas ntawm ntau haiv neeg thiab kev ntsuam xyuas ntawm kev sib raug zoo ntawm cov caj ces hauv molecular biology. AFLP yog ib txoj hauv kev zoo thiab rhiab heev rau kev tshawb nrhiav cov genetic polymorphism ntawm cov kab mob ntau dua li RFLP. Txawm li cas los xij, txawm hais tias ob txoj hauv kev no muaj qhov sib txawv hauv kev tshawb nrhiav kev hloov pauv caj ces, lawv tseem siv rau DNA ntiv tes thiab kuaj kab mob.
