Qhov sib txawv tseem ceeb ntawm bioburden thiab endotoxin yog bioburden yog tus naj npawb ntawm cov kab mob uas muaj nyob hauv cov khoom siv ua ntej ua kom tsis muaj menyuam, thaum endotoxin yog hom tshuaj lom uas tso tawm los ntawm cov kab mob mus rau ib puag ncig ib puag ncig.
Kev kuaj kab mob microbial los yog kev tshuaj ntsuam feem ntau suav nrog kev siv tshuaj lom neeg, tshuaj lom neeg, lossis tshuaj lom neeg los kuaj xyuas thiab txheeb xyuas cov kab mob. Nws feem ntau yog siv los kuaj xyuas cov kab mob ua rau thiab lwj cov kab mob. Kev kuaj microbial tseem ceeb heev rau ntau lub lag luam, cov tib neeg, thiab cov neeg saib xyuas thoob ntiaj teb. Kev kuaj microbial yog qhov tseem ceeb los txheeb xyuas cov kev hem thawj xws li E.coli, Staphylococcus, Pseudomonas, Candida, Aspergillus, thiab lwm yam. Nws kuj pab kom muaj kev lag luam zoo hauv kev lag luam. Bioburden thiab endotoxin yog ob yam tseem ceeb hauv kev kuaj kab mob microbial txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau kis kab mob.
YBioburden yog dab tsi?
Bioburden yog qhov concentration lossis ntau ntawm cov kab mob uas pom los ntawm cov khoom siv ua ntej ua kom tsis muaj menyuam. Lo lus no feem ntau yog siv thaum ua kev sim bioburden (kev kuaj microbial txwv). Kev kuaj Bioburden feem ntau yog ua los ntawm cov khoom siv tshuaj thiab cov khoom siv kho mob rau lub hom phiaj ntawm kev tswj kom zoo. Lub hom phiaj ntawm kev sim bioburden yog ntsuas tag nrho cov kab mob uas siv tau ntawm cov khoom siv tshuaj lossis khoom siv kho mob ua ntej nws qhov kawg ua kom tsis muaj menyuam ua ntej siv.
Daim duab 01: Bioburden
Kev kuaj Bioburden rau cov cuab yeej kho mob tau tsim los ntawm ISO11737 thoob ntiaj teb. Tsis tas li ntawd, Tebchaws Asmeskas Pharmacopeia (USP) qhia txog ntau qhov kev sim uas tuaj yeem ua los txiav txim siab bioburden ntau npaum li cas ntawm cov khoom siv tshuaj tsis muaj menyuam. Thaum ua cov kev ntsuam xyuas no, nws yog ib qho tseem ceeb heev uas lawv tsis txhob qhia cov kab mob microbes rau hauv cov qauv kuaj lossis tua cov kab mob hauv cov qauv kuaj. Nrov bioburden xeem txoj kev rau cov qauv ntawm cov tshuaj muaj xws li cov membrane filtration method thiab phaj suav txoj kev. Bioburden quantification yog ib txwm qhia nyob rau hauv lub colony-forming unit (CFU). Tsis tas li ntawd, bioburden tseem cuam tshuam nrog biofouling. Hauv cov kab ke kev noj qab haus huv, qhov no ua rau muaj kev pheej hmoo ntawm kev noj qab haus huv cuam tshuam nrog kev kis kab mob. Natural convection los yog kua txias hauv chav saib xyuas tseem ceeb, chav huv huv, thiab HEPA cov ntxaij lim dej muaj txiaj ntsig zoo hauv tsev kho mob kom txo cov bioburden.
Endotoxin yog dab tsi?
Endotoxin yog ib hom tshuaj lom uas tso tawm los ntawm cov kab mob mus rau ib puag ncig ib puag ncig. Endotoxins tuaj yeem ua rau tib neeg tuag taus. Nws feem ntau yog los ntawm cov phab ntsa ntawm tes ntawm cov kab mob gram-tsis zoo. Endotoxins muaj ob qho tib si rog thiab cov suab thaj complex. Yog li, endotoxin tseem hu ua lipopolysaccharide hauv cov ntaub ntawv tshawb fawb.
Daim duab 02: Endotoxin
Endotoxins yog pyrogens. Pyrogens yog cov tshuaj ua npaws los ntawm cov kab mob. Thaum muaj ntau txaus, nws tuaj yeem ua rau mob hnyav, poob siab, ntau lub cev tsis ua haujlwm thiab txawm tias tuag rau tib neeg. Kev kuaj kab mob endotoxin tuaj yeem ntsuas qhov muaj thiab ntau ntawm endotoxins hauv ib qho qauv tshwj xeeb.
Dab tsi yog qhov zoo sib xws ntawm Bioburden thiab Endotoxin?
- Bioburden thiab endotoxin yog ob yam tseem ceeb hauv kev kuaj microbial.
- Cov no yuav muaj cov tshuaj tiv thaiv xws li O antigens.
- Ob leeg pab txhawm rau txheeb xyuas qib ntawm cov kab mob microbial thiab txo qis kev pheej hmoo kis mob.
- Lawv tuaj yeem ua rau muaj tshuaj lom thiab ua rau muaj kev mob tshwm sim hauv tib neeg.
Qhov txawv ntawm Bioburden thiab Endotoxin yog dab tsi?
Bioburden yog tus naj npawb ntawm cov kab mob uas muaj nyob hauv cov khoom siv ua ntej ua kom tsis muaj menyuam, thaum endotoxin yog hom tshuaj lom uas tso tawm los ntawm cov kab mob mus rau ib puag ncig ib puag ncig. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm bioburden thiab endotoxin. Bioburden yog ntsuas los ntawm kev kuaj bioburden, thaum endotoxin ntsuas los ntawm kev kuaj endotoxin.
Cov infographic hauv qab no teev qhov sib txawv ntawm bioburden thiab endotoxin hauv daim ntawv tabular rau ib sab sib piv.
Summary – Bioburden vs Endotoxin
Bioburden thiab endotoxin yog ob yam tseem ceeb hauv kev kuaj kab mob microbial txhawm rau txhawm rau txheeb xyuas cov kab mob microbial. Lawv pab txo txoj kev pheej hmoo kis mob. Bioburden yog tus naj npawb ntawm cov kab mob uas muaj nyob hauv cov khoom siv ua ntej ua kom tsis muaj menyuam, thaum endotoxin yog ib hom tshuaj lom uas tso tawm los ntawm cov kab mob mus rau ib puag ncig ib puag ncig. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm bioburden thiab endotoxin.
