Qhov sib txawv tseem ceeb ntawm cov proteinase K thiab protease yog cov proteinase K yog qhov tseem ceeb hauv kev zom cov protein thiab tshem tawm cov kab mob los ntawm nucleic acid npaj, hos protease tseem muaj txiaj ntsig zoo hauv kev ua haujlwm lom neeg xws li kev zom zaub mov noj, protein catabolism, thiab cell signaling..
Proteinase K thiab protease yog ob hom protein-cleaving enzymes. Hauv lwm lo lus, proteinase K thiab protease tuaj yeem tshem peptide bonds hauv cov proteins.
YProteinase K yog dab tsi?
Proteinase K yog qhov dav-spectrum serine protease enzyme. Nws tau pom nyob rau hauv 1974 thiab tau muab rho tawm los ntawm fugus Engyodontium album hom. Cov enzyme no tuaj yeem zom cov keratin hauv cov plaub hau, uas ua rau nws lub npe proteinase K (qhov twg K sawv cev rau keratin). Thaum lub sij hawm kev zom zaub mov no, qhov chaw uas qhov cleavage tshwm sim yog peptide daim ntawv cog lus uas tshwm sim nyob ib sab ntawm pawg carboxyl ntawm aliphatic thiab aromatic amino acids uas tau thaiv cov alpha-amino pawg. Ntxiv mus, qhov enzyme no muaj txiaj ntsig zoo vim nws qhov dav dav ntawm qhov tshwj xeeb.
Thaum xav txog kev ua haujlwm ntawm proteinase K enzyme kev ua haujlwm, nws tau qhib los ntawm calcium. Nws tuaj yeem zom cov protein, feem ntau hydrophobic amino acids xws li aliphatic, aromatic, thiab lwm yam hydrophobic amino acids. Txawm li cas los xij, calcium ions tuaj yeem pab txhawb kev ruaj ntseg ntawm cov enzyme txawm tias cov ions no tsis cuam tshuam rau kev ua haujlwm ntawm enzyme. Tsis tas li ntawd, cov enzyme tuaj yeem zom cov proteins kom tiav yog tias lub sijhawm incubation ntev thiab yog tias cov enzyme concentration siab txaus rau kev zom zaub mov. Yog tias peb tshem cov calcium ions hauv cov tshuaj tiv thaiv sib xyaw, qhov kev ruaj ntseg ntawm cov enzyme txo qis (txawm li cas los xij, cov haujlwm proteolytic tseem tsis hloov).
Muaj ob qhov chaw khi rau calcium ions hauv cov enzyme no. Cov chaw no nyob ze ntawm qhov chaw nquag ntawm cov enzyme, tab sis lawv tsis ncaj qha rau hauv kev ua haujlwm enzymatic. Feem ntau, cov proteinase K enzyme tuaj yeem zom cov proteins uas tuaj yeem paug cov tshuaj nucleic acid npaj; Yog li, peb yuav tsum siv cov enzyme no hauv kev ua kom huv ntawm cov qauv nucleic acid nyob rau hauv lub xub ntiag ntawm EDTA kom txo cov hlau-ion dependent enzymes, xws li. nuclease).
YProtease yog dab tsi?
Protease yog ib qho enzyme catalyzing proteolysis. Nws tseem hu ua peptidase lossis proteinase. Nws tuaj yeem nce tus nqi ntawm cov tshuaj tiv thaiv proteolysis qhov twg cov proteins tau tawg mus rau hauv me me polypeptides los yog rau hauv ib qho amino acids. Lub enzyme ua qhov no catalysis los ntawm cleaving peptide bonds nyob rau hauv cov proteins los ntawm hydrolysis cov tshuaj tiv thaiv (qhov twg cov dej lov bonds).
Qhov no protease enzyme koom nrog ntau yam kev ua haujlwm lom neeg, nrog rau kev zom cov proteins uas tau noj, protein catabolism, thiab cell signaling.
Muaj xya ntau hom protease enzymes raws li hauv qab no:
- YSerine protease
- YCysteine protease
- YThreonine protease
- YGlutamic Protease
- Metalloprotease
- Asparagine peptide lyase
Aspartic Protease Nyob
Qhov txawv ntawm Proteinase K thiab Protease yog dab tsi?
Proteinase K thiab protease yog ob hom protein-cleaving enzymes. Hauv lwm lo lus, proteinase K thiab protease tuaj yeem tshem peptide bonds hauv cov protein. Qhov sib txawv tseem ceeb ntawm cov proteinase K thiab protease yog cov proteinase K yog qhov tseem ceeb hauv kev zom cov protein thiab tshem tawm cov kab mob ntawm nucleic acid npaj, hos protease tseem muaj txiaj ntsig zoo hauv kev ua haujlwm lom neeg xws li kev zom cov protein uas tau noj, protein catabolism, thiab cell signaling.
Cov hauv qab no infographic tabulates qhov sib txawv ntawm proteinase K thiab protease.
Summary – Proteinase K vs Protease
Proteinase K thiab protease yog ob hom protein-cleaving enzymes. Qhov sib txawv tseem ceeb ntawm cov proteinase K thiab protease yog cov proteinase K yog qhov tseem ceeb hauv kev zom cov protein thiab tshem tawm cov kab mob ntawm nucleic acid npaj, hos protease tseem muaj txiaj ntsig zoo hauv kev ua haujlwm lom neeg xws li kev zom cov protein uas tau noj, protein catabolism, thiab cell signaling.
