Qhov sib txawv tseem ceeb ntawm kev ua kom thiab sib txawv ntawm THP1 hlwb yog qhov ua kom THP1 hlwb tsis yog tshwj xeeb, tab sis kev sib txawv ntawm THP1 hlwb yog ib qho txheej txheem tshwj xeeb uas sib txawv ntawm cov cell mus rau hauv ib lub cell zoo li macrophage.
THP1 yog ib kab ntawm cov kab mob monocytic tau los ntawm cov neeg mob ntshav qab zib monocytic. THP1 cell yog ib lub xov tooj loj thiab ncig ib leeg. Cov hlwb no yog siv los kuaj cov kab mob leukemia hauv cov kab mob immunocytochemical ntawm immunohistochemistry thiab protein-protein kev sib cuam tshuam. THP1 hlwb kuj tseem nrov xws li hauv vitro mob qog noj ntshav cell qauv thiab cov qauv los kawm cov txheej txheem monocyte-macrophage physiological txij li lawv muaj cov khoom monocytic. Cov txheej txheem ua kom thiab sib txawv yog qhov tseem ceeb hauv kev txiav txim siab tus cwj pwm ntawm tes thiab nws cov khoom.
Kev ua kom THP1 Cells yog dab tsi?
Kev ua kom ntawm THP1 hlwb tshwm sim tom qab sib txawv rau hauv cov hlwb zoo li macrophage. Qhov no tshwm sim los ntawm ib qho inflammatory stimulus los ntawm kev hloov lawv cov phenotypes mus rau ib qho kev ua kom lub xeev uas hloov cov kev qhia ntawm o ntawm cov noob caj noob ces. Ib txoj hauv kev ua kom THP1 hlwb yog los ntawm kev ua haujlwm metabolic ntawm cov tshuaj hepatotoxic. Kev ua kom ntawm THP1 hlwb yog ib qho tseem ceeb nyob rau hauv peripheral ntshav monocyte qauv. Feem ntau, qhib THP1, uas yog ib qho derivative ntawm THP-1 cell kab, qhia cov yam ntxwv ntawm activated macrophages thaum tsis muaj stimulators. THP-1 kab xov tooj ntawm tes, ib zaug qhib los ntawm kev sib ntxiv ntawm cov stimulators, tsim cov haujlwm macrophage. A-THP-1 yog daim ntawv ua haujlwm ntawm THP-1 cell kab. Hauv qee cov qauv kev tshawb fawb, THP-1 kab xov tooj ntawm tes nthuav qhia cov yam ntxwv ntawm cov macrophages thaum cov stimulators tsis nyob. Cov xwm txheej zoo li no tseem tsis tau ua kom zoo dua. Qhov kev ua haujlwm ntawm THP-1 hlwb ncaj qha cuam tshuam rau kev loj hlob ntawm cov hlwb zoo li macrophage. A-THP-1 cell kab yog lwm txoj hauv kev siv tau rau THP-1 hlwb.
Dab tsi yog qhov txawv ntawm THP1 Cells?
THP1 hlwb sib txawv rau hauv cov hlwb zoo li macrophage, uas qhia cov khoom zoo sib xws rau cov macrophages paub tab. Qhov no tshwm sim ua ntej lub sijhawm ua kom. Cov macrophages no adherent thiab kis tau zoo. THP1 hlwb feem ntau sib txawv los ntawm kev sib ntxiv ntawm phorbol myristate acetate (PMA). Cov txheej txheem sib txawv no tshwm sim los ntawm kev ua kom cov protein kinase nrog phorbol-12-myristate-13acetate (PMA). Qhov no ua rau cov hlwb nrog nce kev ua raws thiab poob ntawm kev ua haujlwm proliferative. Qhov no ua rau muaj kev nce ntxiv hauv phagocytosis ntawm cov hlaws latex thiab kev nthuav qhia ntawm CD14 receptors.
Daim duab 01: Mob Myeloid Leukemia
Differentiation ntawm THP1 hlwb yog ib qho qauv zoo heev rau kev txheeb xyuas txoj hauv kev hauv Lipopolysaccharide-mediated gene activation. Lipopolysaccharide yog ib qho tseem ceeb ntawm cov kab mob gram-tsis zoo. Qhov no ua rau cov ntaub so ntswg raug mob thiab poob siab los ntawm kev ua kom cov monocytes thiab macrophages thiab tso tawm ntawm proinflammatory cytokines xws li IL-1, IL-6, IL-8 thiab TNF-α. Qee cov txheej txheem kev sim ua raws li qhov sib ntxiv ntawm tib neeg gamma interferon (IFN-γ) txhawm rau ua kom muaj kev sib txawv ntawm THP1 cell kab.
Dab tsi yog qhov zoo sib xws ntawm kev ua kom thiab sib txawv ntawm THP1 Cells?
- Cov txheej txheem no tshwm sim ntawm tib lub xov tooj ntawm THP-1.
- Ob qho kev ua kom thiab sib txawv ntawm THP-1 yog qhov tseem ceeb rau qee qhov kev tshawb fawb sim.
- Kev ua kom muaj zog thiab sib txawv ntawm THP-1 hlwb yog siv los kuaj cov kab mob leukemia cell, immunocytochemical kuaj ntawm immunohistochemistry thiab protein-protein kev sib cuam tshuam.
Qhov txawv ntawm qhov ua kom thiab sib txawv ntawm THP1 Cells yog dab tsi?
Kev ua kom THP1 hlwb yog cov txheej txheem tsis tshwj xeeb thaum qhov sib txawv ntawm THP-1 hlwb yog kev kho los ntawm cytokines. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm kev ua kom thiab sib txawv ntawm THP1 hlwb. THP1 hlwb tau sib txawv rau hauv cov hlwb zoo li macrophage. Kev ua kom ua rau txoj kev loj hlob ntawm macrophage ua haujlwm rau qhov sib txawv THP1 hlwb. THP1 hlwb feem ntau yog qhib los ntawm cov tshuaj hepatotoxic thiab sib txawv los ntawm PMA thiab IFN-γ.
Cov lus hauv qab no teev qhov sib txawv ntawm kev ua kom thiab sib txawv ntawm THP1 hlwb rau kev sib piv ntawm ib sab.
Summary – Ua kom vs Sib txawv ntawm THP1 Cells
THP1 cell yog monocytic cell kab hauv tib neeg muab los ntawm cov neeg mob ntshav qab zib monocytic. Kev ua kom thiab sib txawv rau THP1 hlwb tshwm sim vim muaj ntau yam stimulants. Kev sib txawv ua rau THP1 hlwb sib txawv rau hauv cov hlwb zoo li macrophage. Cov kev ua haujlwm macrophage yog induced rau cov hlwb los ntawm kev ua kom. Kev ua kom THP1 hlwb tsis meej. Feem ntau, lawv tau qhib los ntawm cov tshuaj hepatotoxic. Lub caij no, THP1 hlwb sib txawv los ntawm cov cytokines tshwj xeeb los tsim cov hlwb zoo li macrophage. Yog li, qhov no yog cov ntsiab lus ntawm qhov sib txawv ntawm kev ua kom thiab sib txawv ntawm THP1 hlwb.
