Qhov sib txawv tseem ceeb ntawm LAMP thiab PCR kuaj yog tias LAMP tau ua ntawm qhov kub thiab txias (60-65 0 C) los tsim cov ntawv luam ntawm DNA los ntawm kev nthuav dav, thaum PCR tau ua. siv cov kev hloov pauv kub los tsim cov ntawv luam ntawm DNA los ntawm kev nthuav dav.
LAMP thiab PCR yog ob qho tib si hauv vitro amplification cov txheej txheem siv los tsim ntau txhiab daim ntawv luam ntawm DNA. Tus nqi ntawm DNA tsim nyob rau hauv LAMP yog ntau dua li cov nyiaj uas tsim nyob rau hauv PCR cov txheej txheem xws li RT-PCR. LAMP yog cov txheej txheem tshiab dua piv rau cov txheej txheem PCR. Tab sis nws yog technically yooj yim thiab yooj yim rau ib tug kws paub txog kev kawm ua nyob rau hauv lub chaw kuaj mob. Qhov no ua rau nws muaj peev xwm muaj txiaj ntsig zoo rau kev tshawb nrhiav COVID-19. Txawm li cas los xij, RT-PCR yog tus qauv ntsuas tam sim no rau COVID-19. Cov tswv yim no, thaum paub zoo rau cov kws tshawb fawb thiab cov kws kho mob nrog keeb kwm yav dhau los ntawm DNA amplification, tseem yuav tsis paub meej rau cov zej zog dav.
LAMP Test yog dab tsi?
Loop-mediated isothermal amplification (LAMP) rau COVID-19 kev soj ntsuam pib nrog kev sau cov qauv los ntawm lub qhov ntswg lossis caj pas siv lub swab. Cov qauv no tuaj yeem sau tau siv lwm txoj hauv kev xws li hnoos qeev los ntawm kev hnoos hnyav. Zoo li PCR txheej txheem xws li RT-PCR, tus kab mob RNA hauv cov qauv yog thawj zaug hloov mus rau DNA, uas tso cai rau nws luam tawm. Cov tshuaj tiv thaiv LAMP yog ua ntawm qhov kub thiab txias (60-650C).
Qhov kev nthuav dav ntawm cov kab mob DNA sib koom ua ke ntawm LAMP thev naus laus zis thiab cov tshuaj tiv thaiv tuaj yeem tshawb pom thaum cov tshuaj tiv thaiv sib xyaw ua rau huab cua vim yog tsim cov "magnesium pyrophosphate". Qhov huab cua no tso cai rau kev kuaj mob yooj yim ntawm COVID-19 los ntawm cov kws tshawb fawb thiab kws kho mob. Qhov tseeb ntawm cov txiaj ntsig kev ntsuas tuaj yeem txhim kho los ntawm kev siv cov tshuaj fluorescent tshwj xeeb lossis cov xim hloov xim hauv cov tshuaj tiv thaiv sib xyaw. Cov dyes cuam tshuam nrog cov kab mob DNA, thiab qhov kev siv ntawm lub teeb lossis kev hloov xim tuaj yeem ntsuas tau los muab cov naj npawb ntawm cov kab mob RNA molecules kwv yees li qhov pib hauv cov qauv.
Kev kuaj PCR yog dab tsi?
PCR yog cov txheej txheem kev tshawb fawb ntau heev uas tau siv dav hauv kev tshawb fawb thiab tshuaj rau 20-30 xyoo los kuaj DNA. Kev kuaj PCR yog siv ncaj qha los kuaj xyuas cov antigen los ntawm kev kuaj xyuas tus kab mob RNA. Feem ntau, tus kab mob RNA muaj nyob hauv lub cev ua ntej kuaj cov tshuaj tiv thaiv kab mob lossis tau txais cov tsos mob ntawm tus kab mob. Yog li ntawd, kev kuaj PCR tuaj yeem qhia seb puas muaj ib tus neeg muaj tus kabmob no thaum ntxov. Tam sim no, PCR yog qhov ntsuas tus qauv txhawm rau kuaj pom COVID-19. Muaj ntau hom PCR cov txheej txheem xws li lub sijhawm tiag tiag PCR, nested PCR, multiplex PCR, kub pib PCR thiab ntev PCR, thiab lwm yam. PCR siv cov kev hloov pauv kub los tsim cov ntawv luam ntawm DNA los ntawm kev nthuav dav.
RT-PCR yog qhov tshwj xeeb ntawm cov txheej txheem PCR. Nws yog siv thaum kuaj pom RNA. Tam sim no nws yog siv los kuaj COVID-19. RT-PCR yog cov txheej txheem tsim nyog thiab txhim khu kev qha. Hauv RT-PCR, ib zaug sau cov qauv, cov tshuaj siv los tshem tawm cov proteins uas tsis xav tau, rog, thiab lwm yam molecules, tawm hauv RNA tom qab. Cov khoom siv kuaj cov enzymes ua ntej hloov RNA rau DNA, uas tom qab ntawd nthuav dav DNA txhawm rau txhawm rau kuaj tus kab mob. Fluorescent dyes feem ntau yog siv los khi rau cov DNA amplified thiab tsim lub teeb. Qhov no tuaj yeem nyeem los ntawm lub tshuab los ua qhov kev xeem.
Dab tsi zoo sib xws ntawm LAMP thiab PCR Test?
- Lawv yog cov txheej txheem nthuav dav.
- Ob leeg tsim tawm DNA.
- Lawv yog cov txheej txheem rhiab heev thiab txhim khu kev qha ntau dua li cov txheej txheem tiv thaiv kab mob xws li tshuaj tiv thaiv kab mob.
- Lawv ob leeg siv rau kev kuaj COVID-19.
Qhov txawv ntawm LAMP thiab PCR Test yog dab tsi?
Qhov sib txawv tseem ceeb ntawm LAMP thiab PCR kuaj yog tias, hauv LAMP, kev ua kom pom tseeb tau ua tiav siv qhov ntsuas kub tsis tu ncua (60-65 0 C), thaum nyob hauv PCR, kev caij tsheb kauj vab ntawm yuav tsum tau kub. Muaj ntau hom kev teeb tsa LAMP tam sim no. Tab sis qhov tseem ceeb tshaj plaws rau kev kuaj pom COVID-19 yog rov qab hloov pauv cov txheej txheem isothermal isothermal (RT-LAMP) thiab cov ntawv teev lus-kho kom haum (TMA). Tsis tas li ntawd, cov txheej txheem PCR sib txawv kuj tseem muaj nyob rau hauv kev kho mob rau kev kuaj mob xws li PCR real-time, RT-PCR, nested PCR, multiplex PCR, kub pib PCR, ntev PCR, thiab lwm yam.
Cov duab hauv qab no qhia qhov sib txawv ntawm LAMP thiab PCR kuaj hauv daim ntawv teev npe.
Summary – LAMP vs PCR Test
Loop-mediated Isothermal Amplification yog txheej txheem zoo sib xws rau kev kuaj PCR xws li RT-PCR, tab sis nws tsim cov kab mob DNA ntau dua ntawm qhov kub thiab txias. Polymerase saw cov tshuaj tiv thaiv tam sim no yog qhov kev sim ntau tshaj plaws thiab cov qauv hauv ntiaj teb rau kev kuaj mob, xws li kev kuaj mob ntawm COVID-19. Nws yog pom raws li kev ntseeg ncaj ncees. PCR siv cov kev hloov pauv kub los tsim cov ntawv luam ntawm DNA los ntawm kev nthuav dav. Yog li, qhov no qhia txog qhov sib txawv ntawm LAMP thiab PCR xeem.
