Qhov sib txawv tseem ceeb ntawm kev txhim kho thiab kev ruaj khov yog qhov kev txhim kho muaj kev nkag mus sai ntawm kev txhim kho reagent rau hauv cov ntaub so ntswg thiab kho cov ntaub so ntswg nrog cov qauv biomolecular uas twb muaj lawm, qhov kev ruaj ntseg cuam tshuam nrog qhov kawg ntawm cov txheej txheem fixation thiab optimally tiv thaiv biomolecules ntev. lub sijhawm.
Kho thiab stabilization yog cov txheej txheem tseem ceeb hauv biochemistry rau kev khaws cov ntaub so ntswg thiab kev coj noj coj ua.
Kev kho yog dab tsi?
Fixation yog cov txheej txheem tshuaj xyuas uas cov khoom siv ntawm cov hlwb thiab cov ntaub so ntswg raug kho nyob rau hauv lub cev thiab ib nrab tshuaj hauv lub xeev txhawm rau ua kom lawv tiv taus cov kev kho mob tom ntej, suav nrog ntau cov tshuaj reagents. Hauv cov txheej txheem no, qhov poob ntawm reagents yog qhov tsawg kawg nkaus, thiab muaj qhov cuam tshuam loj lossis decomposition.
Thaum cov ntaub so ntswg raug tshem tawm ntawm lub cev, nws nyiam mus los ntawm tus txheej txheem ntawm kev puas tsuaj rau tus kheej, uas yog hu ua autolysis. Yog li ntawd, yog tias peb tawm hauv cov ntaub so ntswg yam tsis muaj kev khaws cia, kab mob tuaj yeem tshwm sim (qhov no hu ua putrefaction). Txhawm rau zam cov txheej txheem no, yuav tsum tau khaws cia thiab ua kom tawv nqaij ntawm cov ntaub so ntswg, ua kom zoo zoo ib yam li cov ntaub so ntswg nyob ze li sai tau.
Cov txheej txheem no yog ib qho tseem ceeb los tiv thaiv autolysis thiab putrefaction, tseem ceeb rau kev nrawm thiab txawm tias nkag mus, rau kev khaws cia ntawm cov hlwb thiab cov ntaub so ntswg kom ua neej nyob raws li qhov ua tau, kom ruaj khov cov ntsiab lus, thiab lwm yam.
Daim duab 01: Kev khaws cov ntaub so ntswg
Muaj ntau txoj kev sib txawv uas peb tuaj yeem siv los kho. Qhov no suav nrog kev kho cua sov, siv cov tshuaj xws li coagulants, thiab lwm yam. Peb tuaj yeem faib cov tshuaj kho mob ntau tshaj plaws rau ntau pawg xws li aldehydes, oxidizing agents, protein denaturing agents, cross-linking agents, thiab lwm yam.
Ntxiv mus, muaj ntau yam uas cuam tshuam rau kev kho, xws li hydrogen ion concentration, kub, nkag mus, osmolality, thiab concentration duration.
Kev ruaj ntseg yog dab tsi?
Stabilization yog cov txheej txheem tshuaj xyuas uas muaj txiaj ntsig zoo rau kev tso tseg cov txheej txheem kho thiab tiv thaiv biomolecules zoo rau kev khaws cia ntev. Yog li ntawd, txoj kev no los tom qab lub fixation kauj ruam. Nrog cov stabilizers xws li PAXgene Tissue Stabilizer, peb tuaj yeem tiv thaiv peb cov qauv ntaub so ntswg ntev li 7 hnub ntawm chav tsev kub, thiab peb tuaj yeem khaws lawv mus txog 4 lub lis piam ntawm qhov kub thiab txias. Yog tias qhov ntsuas kub qis dua, ces peb tuaj yeem khaws cov ntaub so ntswg rau ntau xyoo.
Kev ruaj khov tam sim ntawm cov ntaub so ntswg kuj tseem ceeb rau khaws cia hauv vivo profile ntawm DNA< RNA thiab cov proteins. Feem ntau ntawm cov stabilizers peb siv niaj hnub no yog formalin-free preservation uas muab kev txhim kho molecular tau los ntawm cov ntaub so ntswg ruaj.
Dab tsi yog qhov txawv ntawm Fixation thiab Stabilization?
Kho thiab ruaj khov yog cov txheej txheem kev tshuaj xyuas tseem ceeb. Qhov sib txawv tseem ceeb ntawm fixation thiab stabilization yog tias fixation muaj kev nkag mus sai ntawm fixation reagent rau hauv cov ntaub so ntswg thiab kho cov ntaub so ntswg nrog biomolecular qauv uas twb muaj lawm, whereas lub stabilization txheej txheem yuav xaus ntawm lub fixation txheej txheem thiab optimally tiv thaiv biomolecules rau lub sij hawm ntev. Tsis tas li ntawd, kev txhim kho muaj cov txheej txheem kev kho cua sov thiab cov txheej txheem tshuaj (xws li cov tshuaj coagulant thiab tsis yog coagulant chemicals) thaum stabilization yuav tsum khov kom qis qis.
Cov hauv qab no yog cov ntsiab lus ntawm qhov sib txawv ntawm kev kho thiab ruaj khov hauv daim ntawv teev npe.
Summary – Fixation vs Stabilization
Kho thiab stabilization yog cov txheej txheem tseem ceeb hauv biochemistry rau kev khaws cov ntaub so ntswg thiab kev coj noj coj ua. Qhov sib txawv tseem ceeb ntawm fixation thiab stabilization yog tias fixation yuav nkag mus sai sai ntawm fixation reagent rau hauv cov ntaub so ntswg thiab kho cov ntaub so ntswg nrog biomolecular qauv uas twb muaj lawm, whereas cov txheej txheem stabilization yuav xaus ntawm fixation txheej txheem thiab optimally tiv thaiv biomolecules rau lub sij hawm ntev.
