Qhov sib txawv tseem ceeb ntawm buffered unbuffered thiab neutralized formalin yog tias buffered formalin ua raws li qib zoo tshaj plaws formalin khaws cov ntaub so ntswg, whereas unbuffered formalin thiab neutralized formalin qhia tsis zoo khaws cia cov ntaub so ntswg.
Formalin yog cov tshuaj tsis muaj xim ntawm formaldehyde hauv dej. Nws yog ib qho organic compound uas tshwm sim ib txwm muaj, thiab nws muaj cov tshuaj formula CH2O-(H-CHO). Pure formaldehyde muaj cov ntxhiab tsw ntxhiab, thiab nws feem ntau tshwm sim raws li cov ntxhiab tsw tsis muaj xim uas tuaj yeem ua rau polymerization ntawm tus kheej, tsim paraformaldehyde.
Formalin tuaj yeem pom hauv peb hom loj: buffered, unbuffered thiab neutralized forms. Peb pawg no tau muab faib raws li qhov muaj peev xwm buffering ntawm cov kev daws teeb meem no. Peb qib ntawm formalin no tuaj yeem siv rau kev tshawb fawb txog formalin fixatives. Formalin fixatives yog cov tshuaj daws teeb meem uas peb tuaj yeem siv los khaws cov khoom ntawm cov khoom muaj sia xws li tsiaj lossis cov ntaub so ntswg. Feem ntau cov formalin fixative siv nyob rau hauv lub chaw soj nstuam yog buffered formalin fixative.
Dab tsi yog Buffered Formalin?
Buffered formalin yog tus qauv thiab nyiam formalin fixative rau cov ntaub so ntswg preservation. Cov tshuaj no feem ntau yog yuav los ua cov tshuaj npaj, thiab nws zam qhov kev pheej hmoo ntxiv ntawm kev tuav yuav tsum tau sib tov cov tshuaj buffered formalin los ntawm cov tshuaj hauv Tshuag. Feem ntau, cov tshuaj buffered formalin no yog npaj los ntawm kev sib tov ib feem ntawm cov khoom formalin nrog 9 qhov chaw ntawm cov dej distilled. Txhawm rau kom tau txais lub peev xwm buffering, peb tuaj yeem ntxiv reagents xws li monobasic sodium hypophosphate thiab dibasic lossis anhydrous sodium hyperphosphate. Txawm li cas los xij, muaj lwm txoj hauv kev uas suav nrog kev sib ntxiv ntawm sodium chloride thiab dibasic sodium hyper phosphate rau kev sib xyaw ntawm formalin thiab dej rau qhov sib piv ntawm 1: 9.
Thaum cov txheej txheem ntawm cov ntaub so ntswg khaws cia hauv cov ntaub so ntswg, peb tuaj yeem pom tias cov ntaub so ntswg tau khaws cia zoo, qhia qhov kev puas tsuaj tsawg kawg rau cov ntaub so ntswg. Hom kev daws teeb meem no tseem ceeb heev hauv kev xav tau kev cia ntev ntev thiab cov chaw soj nstuam loj.
Dab tsi yog Unbuffered Formalin?
Unbuffered formalin yog ib qho kev daws ntawm formalin hauv dej. Hom kev daws teeb meem no yog tsim thaum ib feem ntawm formalin tov nrog 9 feem ntawm dej. Cov tshuaj sib tov no muaj pH txog 3-4 uas tuaj yeem sib txawv raws li qhov concentration ntawm formalin Tshuag peb siv rau lub hom phiaj no.
Vim tias cov tshuaj formalin uas tsis muaj tshuaj muaj kua qaub (pH thaj tsam ntawm 3 txog 4), cov kua qaub tuaj yeem ua rau muaj kev cuam tshuam ntawm hemoglobin hauv cov ntaub so ntswg uas peb yuav khaws cia, thiab nws tuaj yeem tsim cov xim av xim av formaldehyde. haematin precipitate uas tuaj yeem cuam tshuam kev txhais lus histological.
Neutralized formalin yog dab tsi?
Neutralized formalin yog ib qho kev daws teeb meem ntawm formalin hauv dej, muaj tus nqi nruab nrab pH. Yog li, pH ntawm hom tshuaj no yuav tsum yog 7.0. Thaum cov tshuaj formalin tov nrog dej, nws muab cov kua qaub, yog li peb yuav tsum tau kho cov pH siv lub hauv paus xws li sodium hydroxide.
Qhov txawv ntawm Buffered Unbuffered thiab Neutralized Formalin yog dab tsi?
Formalin tuaj yeem pom hauv peb hom loj: buffered, unbuffered thiab neutralized forms. Qhov sib txawv tseem ceeb ntawm buffered unbuffered thiab neutralized formalin yog tias buffered formalin ua raws li qib zoo tshaj plaws formalin khaws cov ntaub so ntswg, hos unbuffered formalin thiab neutralized formalin qhia tsis zoo khaws cov ntaub so ntswg.
Cov duab hauv qab no qhia cov ntsiab lus ntau ntxiv ntawm qhov sib txawv ntawm qhov sib txawv ntawm qhov sib txawv ntawm qhov tsis sib haum xeeb thiab nruab nrab formalin hauv daim ntawv tabular rau ib sab ntawm kev sib piv.
Summary – Buffered vs Unbuffered vs Neutralized Formalin
Formalin fixatives yog cov tshuaj tiv thaiv uas peb tuaj yeem siv los khaws cov ntaub so ntswg, thiab lwm yam, hauv chav kuaj. Muaj peb hom formalin fixatives uas peb tuaj yeem siv hauv peb cov kev tshawb fawb: buffered, unbuffered thiab neutralized formalin. Qhov sib txawv tseem ceeb ntawm buffered unbuffered thiab neutralized formalin yog tias buffered formalin ua raws li qib zoo tshaj plaws formalin khaws cov ntaub so ntswg, hos unbuffered formalin thiab neutralized formalin qhia tsis zoo khaws cov ntaub so ntswg.
