Qhov sib txawv tseem ceeb ntawm immortalized thiab transformed cells yog cov hlwb immortalized tsis yog mob qog noj ntshav, thaum hloov hlwb yog mob qog noj ntshav.
Cov hlwb hloov pauv thiab cov hlwb tsis txawj tuag yog ob hom hlwb. Lawv faib tsis tu ncua. Cov hlwb immortalized muaj lub neej tsis kawg. Hloov cov hlwb qhia tag nrho cov cim ntawm cov qog nqaij hlav cancer. Lawv tuaj yeem tsim cov cell loj (cov qog). Ob qho tib si immortalization thiab kev hloov pauv yog cov xwm txheej tseem ceeb ntawm kev tsim mob qog noj ntshav. Txawm li cas los xij, tsis zoo li cov hlwb uas tsis txawj tuag, cov hlwb hloov pauv tau nthuav tawm cov cell proliferation thiab invasiveness.
Dab tsi yog Immortalized Cells?
Immortalized cells yog cov hlwb uas muaj peev xwm faib tau mus ib txhis. Hauv lwm lo lus, cov hlwb tsis txawj tuag yog cov hlwb uas muaj lub neej tsis kawg. Feem ntau, cov hlwb ib txwm muaj lub neej tsis kawg, tab sis cov hlwb tsis txawj tuag muaj lub neej tsis kawg. Lawv muaj peev xwm tsim cov kab txuas txuas ntxiv. Yog li ntawd, immortalized hlwb tau evaded senescence. Qhov tseem ceeb tshaj plaws, nws ntseeg tau tias kev tsis txawj tuag yuav tshwm sim thaum qee qhov kev tswj hwm ntawm tes tsis ua haujlwm. Cov hlwb immortalized tau hloov pauv txaus kom muaj lub neej tsis kawg. Txawm li cas los xij, tsis zoo li cov hlwb hloov pauv, cov hlwb immortalized tsis yog mob qog noj ntshav. Lawv qhia txog kev vam khom rau kev loj hlob yam tseem ceeb thiab muaj kev cuam tshuam rau kev loj hlob inhibitors ib yam nkaus.
Daim duab 01: Cov Cells tsis txawj tuag
Cells tuaj yeem tau txais kev tsis txawj tuag ntawm tus kheej; nws tseem tuaj yeem tsim nyob rau hauv chav kuaj. Immortalized hlwb qhia ntau yam zoo. Feem ntau, lawv tau siv los ua cov kab ntawm tes hauv ntau lub chaw kuaj mob. Cov hlwb immortalized yog homogenous thiab genetically zoo tib yam cell pejxeem. Yog li ntawd, lawv tsim cov txiaj ntsig zoo dua qub. Lawv kuj yog ib qho yooj yim rau kab lis kev cai nyob rau hauv cov chaw kuaj mob. Ntxiv mus, nws tsis yog yuav tsum tau rho tawm lawv los ntawm ib tug tsiaj nyob. Immortalized cell kab loj hlob sai thiab tuaj yeem nthuav qhia cov noob ntawm kev txaus siab tsis tu ncua. Yog li, lawv tuaj yeem siv los rho tawm cov protein ntau rau kev kuaj biochemical. Cov hlwb HeLa yog ib hom ntawm cov hlwb tsis txawj tuag thiab lawv tau siv dav los kuaj cov tshuaj tshuaj thiab tsim cov tshuaj tiv thaiv. Tsis tas li ntawd, cov hlwb immortalized tuaj yeem siv rau cov tshuaj tiv thaiv monoclonal. Tab sis, qhov tsis zoo loj yog cov hlwb uas tsis txawj tuag tsis tuaj yeem suav tias yog cov hlwb ib txwm muaj.
Dab tsi yog Transformed Cells?
Kev hloov pauv yog txheej txheem tseem ceeb rau kev tsim cov qog nqaij hlav cancer. Transformation tso cai rau cov hlwb los ua uncoupled los ntawm kev tswj mechanisms. Nws kuj tso cai rau cov cell loj hlob sai thiab invasively, qhia indefinite proliferation. Hloov cov hlwb muaj tag nrho cov yam ntxwv ntawm cov qog nqaij hlav cancer. Yog li ntawd, lawv yog cov qog nqaij hlav cancer. Lawv tuaj yeem proliferate tsis tu ncua, tsim cov cell loj, tshwj xeeb tshaj yog cov qog. Lawv qhia kev ywj pheej los ntawm kev loj hlob.
Daim duab 02: Hloov cov Cells
Ntxiv mus, cov hlwb hloov pauv tsis teb rau kev loj hlob inhibitors. Lawv tuaj yeem tiv thaiv apoptosis. Tsis tas li ntawd, lawv tsis nkag mus rau theem senescence. Qhov tseem ceeb tshaj plaws, lawv tuaj yeem txhawb nqa angiogenesis. Lawv kuj yog cov kab mob invasive. Hloov cov hlwb qhia kev ywj pheej ntawm kev ywj pheej, thiab cov hlwb loj hlob hauv kev tsis sib haum xeeb. Cov hlwb hloov pauv kuj yog qhov tshwm sim los ntawm kev poob ntawm kev sib cuag inhibition.
Dab tsi yog qhov zoo sib xws ntawm Immortalized thiab Transformed Cells?
- Txhua lub hlwb tsis txawj tuag thiab hloov pauv tsis pom qhov tsis zoo.
- Lawv tuaj yeem faib tsis tas li.
- Immortalization thiab transformation yog ob txheej xwm ntawm oncogenesis.
- Txhua yam kev hloov pauv thiab kev tsis txawj tuag tuaj yeem tshwm sim ntawm tus kheej lossis vim muaj kab mob kis.
Qhov txawv ntawm Immortalized thiab Transformed Cells yog dab tsi?
Immortalized cells faib tsis tu ncua, thiab lawv muaj lub neej tsis kawg. Hloov pauv cov hlwb tau txhim kho cell proliferation muaj peev xwm thiab invasiveness. Yog li, cov hlwb hloov pauv yog cov qog nqaij hlav cancer, thaum cov hlwb tsis txawj tuag tsis yog cov qog nqaij hlav cancer. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm immortalized thiab hloov pauv hlwb. Ntxiv mus, immortalized hlwb qhia dependence rau kev loj hlob yam, thiab lawv rhiab heev rau kev loj hlob inhibitors. Ntawm qhov tod tes, cov hlwb hloov pauv qhia qhov kev loj hlob ntawm kev ywj pheej, thiab lawv tsis pom cov lus teb rau kev loj hlob inhibitors. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm cov hlwb uas tsis txawj tuag thiab hloov pauv.
Cov hauv qab no infographic qhia txog qhov sib txawv ntawm cov hlwb tsis txawj tuag thiab hloov pauv hauv daim ntawv teev npe.
Summary – Immortalized vs Transformed Cells
Immortalized cells tuaj yeem faib ua ntu zus. Lawv muaj lub neej tsis kawg. Tab sis, immortalized hlwb tsis yog mob qog noj ntshav. Cov hlwb hloov pauv yog cov qog nqaij hlav qog noj ntshav, thiab lawv qhia txog kev loj hlob ntawm tes muaj peev xwm thiab invasiveness. Tsis tas li ntawd, cov hlwb hloov pauv qhia tias muaj kev ywj pheej thiab kev tiv thaiv kev sib cuag. Yog li, qhov no yog cov ntsiab lus ntawm qhov sib txawv ntawm immortalized thiab transformed hlwb.
