Qhov sib txawv tseem ceeb ntawm phycocyanin thiab allophycocyanin yog tias phycocyanin absorbs thiab tawm ntawm lub wavelengths luv dua li allophycocyanin.
Phycobiliproteins yog tsev neeg ntawm cov dej-soluble proteins uas muaj nyob rau hauv cyanobacteria thiab qee hom algae. Phycocyanin thiab allophycocyanin yog ob tug tswv cuab tseem ceeb ntawm tsev neeg no.
Phycocyanin yog dab tsi?
Phycocyanin yog cov xim-protein complex los ntawm tsev neeg ntawm lub teeb-sau phycobiliprotein. Lwm cov tswv cuab tseem ceeb ntawm tsev neeg no suav nrog allophycocyanin thiab phycoerythrin. Cov xim no yog ib qho xim ntxiv rau chlorophyll. Feem ntau, tag nrho cov phycobiliproteins yog cov dej-soluble complexes uas tsis tuaj yeem nyob hauv daim nyias nyias xws li carotenoids. Hloov chaw uas muaj nyob rau hauv daim nyias nyias, cov xim no zoo li sib sau ua ke, tsim cov pawg uas tuaj yeem ua raws li daim nyias nyias hu ua phycobilisomes.
Daim duab 01: Extracted Phycocyanin Pigments (los ntawm Cyanobacteria)
Peb tuaj yeem pom tias Phycocyanin muaj cov yam ntxwv lub teeb xiav uas tuaj yeem nqus cov txiv kab ntxwv thiab lub teeb liab (ze 620 nm) thiab tuaj yeem tawm fluorescence (ze li 650 nm). Peb tuaj yeem pom cov xim xim no hauv cyanobacteria thiab lub npe "Phycocyanin" los ntawm Greek lub ntsiab lus rau "Phyco" uas yog hais txog "algae" thiab cov lus "cyanin" los ntawm Greek lub ntsiab lus rau "Kyanos" uas yog hais txog "dub xiav".
Feem ntau, Phycocyanin molecules sib koom ua ke nrog txhua tus phycobiliproteins. Thaum xav txog cov qauv ntawm cov xim no, nws pib nrog kev sib dhos ntawm phycobiliprotein monomers. Cov monomers no yog heterodimers uas muaj alpha thiab beta subunits nrog lawv cov chromophores. Cov chromophores thiab cov subunits tau koom ua ke los ntawm thioether chemical bond.
Cov subunits ntawm Phycocyanin qauv feem ntau muaj yim alpha-helices. Cov qauv monomer zoo li spontaneously aggregate, tsim lub nplhaib-shaped trimers muaj rotational symmetry thiab lub hauv paus channel. Ntxiv mus, cov trimers zoo li sib sau ua ke, tsim hexamers uas tau pab nrog ntxiv cov protein txuas ntxiv. Yog li ntawd, txhua tus pas nrig phycobilisome muaj ob lossis ntau dua Phycocyanin hexamers.
Allophycocyanin yog dab tsi?
Allophycocyanin yog ib qho protein molecule uas los ntawm tsev neeg phycobiliprotein, thiab nws yog ib qho khoom siv xim rau chlorophyll. Lwm cov tswvcuab ntawm tsev neeg phycobiliprotein lub teeb ci no suav nrog Phycocyanin, phycoerythrin, thiab phycoerythrocyanin. Allophycocyanin pigments tuaj yeem nqus tau thiab tawm lub teeb liab, thiab peb tuaj yeem pom cov xim no hauv cyanobacteria thiab liab algae.
Daim duab 02: Qhov tshwm sim ntawm Allophycocyanin hauv daim duab
Peb tuaj yeem cais cov allophycocyanin los ntawm ntau hom liab lossis xiav-ntsuab algae. Cov algae no tsim cov qauv sib txawv me ntsis ntawm cov molecule. Feem ntau, allophycocyanin molecule muaj ob lub subunits sib txawv npe hu ua alpha thiab beta subunit. Txhua lub subunit muaj ib qho phycocyanobilin chromophore.
Muaj ntau daim ntawv thov ntawm allophycocyanin; ntau cov twj paj nruag tau tsim tshwj xeeb rau allophycocyanin. Piv txwv li, cov khoom no feem ntau yog siv rau hauv immunoassays, suav nrog FACS, flow cytometry, thiab lwm yam.
Dab tsi zoo sib xws ntawm Phycocyanin thiab Allophycocyanin?
- Phycocyanin thiab allophycocyanin yog dej-soluble proteins
- Ob leeg suav nrog hauv tsev neeg phycobiliprotein.
Qhov txawv ntawm Phycocyanin thiab Allophycocyanin yog dab tsi?
Phycobiliproteins yog tsev neeg ntawm cov dej-soluble proteins uas muaj nyob rau hauv cyanobacteria thiab qee hom algae. Phycocyanin thiab allophycocyanin yog ob tug tswv cuab tseem ceeb ntawm tsev neeg no. Qhov sib txawv tseem ceeb ntawm phycocyanin thiab allophycocyanin yog Phycocyanin absorbs thiab emits ntawm luv wavelengths tshaj allophycocyanin.
Hauv qab infographic teev ntau qhov sib txawv ntawm phycocyanin thiab allophycocyanin hauv daim ntawv teev npe rau ib sab ntawm kev sib piv.
Summary – Phycocyanin vs Allophycocyanin
Phycobiliproteins yog tsev neeg ntawm cov dej-soluble proteins uas muaj nyob rau hauv cyanobacteria thiab qee hom algae. Phycocyanin thiab allophycocyanin yog ob tug tswv cuab tseem ceeb ntawm tsev neeg no. Qhov sib txawv tseem ceeb ntawm Phycocyanin thiab allophycocyanin yog Phycocyanin absorbs thiab emits ntawm luv wavelengths tshaj allophycocyanin.
