Qhov sib txawv tseem ceeb ntawm electroporation thiab microinjection yog tias electroporation yog cov txheej txheem uas siv lub zog hluav taws xob hluav taws xob kom xa DNA mus rau hauv cov hlwb thaum microinjection yog cov txheej txheem uas siv lub koob iav zoo los yog micropipette xa DNA mus rau hauv. host cells.
Kev hloov pauv yog txheej txheem uas txawv teb chaws DNA hloov mus rau cov tswv tsev. Los ntawm kev hloov pauv, cov genetic pleev ntawm lub cev tuaj yeem hloov pauv. Muaj ntau txoj kev hloov tshuaj lom neeg, lom neeg thiab lub cev hloov pauv. Qee qhov yog txoj hauv kev ncaj qha, thaum qee qhov yog txoj hauv kev tsis ncaj. Electroporation thiab microinjection yog ob txoj hauv kev ntawm lub cev uas yog txoj kev hloov pauv ncaj qha. Electroporation siv lub teb hluav taws xob los ua kom cov kab mob microscopic pores nyob rau hauv cov cell membranes thiaj li koom nrog DNA rau hauv cov tswv tsev. Microinjection, ntawm qhov tod tes, ncaj qha xa DNA siv lub micropipette lossis rab koob zoo-tipped iav.
Electroporation yog dab tsi?
Electroporation yog cov txheej txheem hloov pauv uas xa DNA rau hauv cov nroj tsuag thiab cov protoplasts. Cov txheej txheem no siv lub zog hluav taws xob hluav taws xob siab. Cov ntaub ntawv cog yog incubated nyob rau hauv ib qho kev daws teeb meem tsis muaj DNA. Tom qab ntawd qhov kev daws teeb meem yog raug rau lub siab voltage hluav taws xob mem tes. High voltage induced pores yog tsim nyob rau hauv cov nroj tsuag cell membranes, thiab los ntawm cov pores, DNA migrates nyob rau hauv lub hlwb thiab integrates nrog cog genomic DNA. Kev ua tau zoo ntawm txoj kev no nyob ntawm cov ntaub ntawv cog thiab kev kho mob.
Daim duab 01: Electroporation
Thaum kev hloov pauv tau ua tiav siv electroporation, tsuas yog 40 txog 50% ntawm cov hlwb tau txais DNA. Ntxiv mus, tsuas yog 50% ntawm cov hlwb hloov pauv tuaj yeem muaj sia nyob raws li txoj kev no. Txawm li cas los xij, txoj kev no yooj yim rau kev ua thiab tsis hloov cov qauv lom neeg lossis kev ua haujlwm ntawm cov hlwb. Ntxiv mus, nws tuaj yeem siv rau ntau lub hlwb.
Microinjection yog dab tsi?
Microinjection yog cov txheej txheem hloov pauv uas muaj txiaj ntsig tshwj xeeb thaum qhia DNA rau hauv cov hlwb loj. Txoj kev no siv lub koob iav zoo los yog micropipette xa DNA mus rau hauv cov nroj tsuag protoplasts lossis tsiaj hlwb (oocytes, qe thiab embryos). Qhov tseeb, txoj kev no yog qhov tsim nyog rau kev ua cov tsiaj transgenic xws li nas. Hauv txoj kev no, DNA tau koom ncaj qha rau hauv lub nucleus lossis cytoplasm.
Zoo ib yam li electroporation, microinjection yog ib qho kev hloov pauv ncaj qha. Microinjection yog ua nyob rau hauv ib tug tshwj xeeb microscope teeb. Computerized tswj tuav pipette, koob, microscope theem thiab yees duab technology tau nce qhov ua tau zoo ntawm cov txheej txheem no. Thaum txhaj DNA, cov xim tuaj yeem siv los txheeb xyuas cov hlwb hloov tau yooj yim. Yog li ntawd, tsis tas yuav siv txoj kev sib cais los txheeb xyuas cov hlwb hloov pauv. Qhov tseem ceeb tshaj, cov txheej txheem microinjection tsis tas yuav muaj cov cim cim npe.
Daim duab 02: Microinjection
Ntxiv mus, txoj kev no yog qhov zoo heev thiab rov tsim dua. Txawm li cas los xij, txoj kev no yog kim, siv sijhawm thiab xav tau tus neeg txawj ntse. Tsis tas li ntawd xwb, tsuas yog ib qho me me ntawm cov hlwb tuaj yeem kho nrog txoj kev no.
Dab tsi yog qhov zoo sib xws ntawm Electroporation thiab Microinjection?
- Ob qho tib si electroporation thiab microinjection yog ob hom kev hloov pauv.
- Cov txheej txheem no xa DNA rau hauv cov nroj tsuag thiab cov protoplasts.
- Lawv yog txoj kev ncaj.
- Ntxiv mus, lawv yog lub cev lossis kev siv tshuab.
- Ob txoj kev no pab tau thaum tsim cov nroj tsuag thiab tsiaj txhu.
Qhov txawv ntawm Electroporation thiab Microinjection yog dab tsi?
Electroporation cov txheej txheem siv hluav taws xob los qhia DNA thaum cov txheej txheem microinjection yog micropipette lossis rab koob zoo-tipped iav los qhia DNA. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm electroporation thiab microinjection. Ntxiv mus, electroporation feem ntau yog siv rau cov nroj tsuag hlwb thiab protoplasts thaum microinjection feem ntau yog siv rau cov tsiaj hlwb. Tsis tas li ntawd, electroporation tsis yog siv sijhawm ntev li microinjection.
Cov hauv qab no infographic qhia txog qhov sib txawv ntawm electroporation thiab microinjection hauv daim ntawv tabular.
Summary – Electroporation vs Microinjection
Electroporation thiab microinjection yog ob txoj hauv kev ntawm lub cev hloov cov noob. Electroporation siv cov hluav taws xob hluav taws xob siab thaum microinjection siv lub khob koob lossis micropipette. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm electroporation thiab microinjection. Txawm li cas los xij, ob txoj hauv kev ncaj qha qhia exogenous DNA mus rau hauv cov hlwb tswv.
