Qhov sib txawv tseem ceeb ntawm cytotoxicity thiab genotoxicity yog tias cytotoxicity yog qhov ua tau zoo ntawm cov tshuaj lom rau cov hlwb thaum genotoxicity yog lub peev xwm ua rau DNA thiab / lossis cov cuab yeej cellular uas tswj kev ncaj ncees ntawm genome.
Cytotoxicity thiab genotoxicity yog ob yam zoo ntawm cov tshuaj los yog tshuaj. Cytotoxicity yog qhov zoo ntawm cov tshuaj lom rau cov hlwb thaum genotoxicity yog qhov zoo ntawm kev puas tsuaj rau cov ntaub ntawv caj ces hauv ib lub cell, ua rau kev hloov pauv. Qee cov tshuaj lom neeg tuaj yeem ua rau cytotoxicity thiab genotoxicity. Tsis tas li ntawd, genotoxicity tuaj yeem ua rau cytotoxicity. Ib qho ntxiv, cytotoxicity yuav los yog tsis yog genotoxic vim tsis yog txhua tus neeg sawv cev cytotoxic yog genotoxic.
YCytotoxicity yog dab tsi?
Cytotoxicity yog cov cuab yeej ntawm cov tshuaj lom neeg rau cov hlwb. Yog li, yog tias koj kho cov hlwb nrog tus neeg sawv cev cytotoxic, nws tuaj yeem rhuav tshem cov hlwb. Cov tshuaj no tuaj yeem ua rau lossis tsis ua rau cov genome lossis cov khoom siv caj ces ntawm lub cell. Yog li, tsis yog txhua tus neeg sawv cev cytotoxic yog genotoxic. Thaum kho nrog tus neeg sawv cev cytotoxic, cov hlwb yuav raug mob necrosis, thiab lawv yuav tuag sai vim yog cell lysis. Tsis tas li ntawd, cov cell yuav tsum tsis txhob loj hlob thiab faib. Cov tshuaj cytotoxic kuj tseem ua rau apoptosis lossis programmed cell tuag. Hauv cov neeg mob qog noj ntshav, kws khomob feem ntau siv tshuaj cytotoxic los tua lossis puas tsuaj sai, faib cov qog nqaij hlav cancer. Yog li, cytotoxicity yog ib qho tseem ceeb uas tau txiav txim siab thaum tsim cov tshuaj tiv thaiv kab mob cancer.
Daim duab 01: Cov Cells Muaj Necrosis thiab Apoptosis
Cytotoxicity tuaj yeem ntsuas tau siv cov kev ntsuas sib txawv xws li kev tshuaj xyuas cov xim tsis suav nrog cov tshuaj pleev xim xim, cov tshuaj fluorometric thiab cov tshuaj luminometric. Nws yog ib qho tsim nyog los xaiv txoj kev tsim nyog. Lub cell membrane kev ncaj ncees qhia tau hais tias cell viability thiab cytotoxic los ntawm cov tshuaj. Yog li ntawd, kev ntsuam xyuas cell membrane kev ncaj ncees yog txoj kev zoo tshaj plaws los ntsuas cytotoxicity. Cov tshuaj cytotoxic feem ntau cuam tshuam cov cell membrane kev ncaj ncees. Cov hlwb noj qab haus huv tsis pub trypan xiav lossis propidium iodide nkag mus rau hauv lub cell. Thaum lub cell poob cell membrane kev ncaj ncees, trypan xiav los yog propidium iodide mus rau hauv lub hlwb thiab stain rau sab hauv. Ntxiv mus, cytotoxicity tuaj yeem ntsuas tau siv 3-(4, 5-Dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide (MTT) lossis los ntawm sulforhodamine B (SRB) kev soj ntsuam. Nyob rau hauv cov ntshav los yog cov pob txha pob txha, ib qho kev hloov tseem ceeb nyob rau hauv qhov kev faib ua feem ntawm erythrocytes tsis paub tab ntawm tag nrho cov erythrocytes qhia lub cytotoxicity ntawm cov tshuaj.
Dab tsi yog Genotoxicity?
Genotoxicity yog lub peev xwm ua rau DNA ntawm lub cell puas. Hauv lwm lo lus, genotoxicity yog lub peev xwm ntawm cov tshuaj lom neeg los rhuav tshem cov khoom siv caj ces ntawm lub xov tooj ntawm tes thiab ua rau kev hloov pauv (hloov cov ntaub ntawv ntawm caj ces). Thaum DNA puas lawm, nws tuaj yeem ua rau mob qog noj ntshav, kab mob caj ces lossis kev yug menyuam. Ntxiv mus, nws tuaj yeem ua rau cytotoxicity thiab rhuav tshem cov hlwb. Feem ntau, tag nrho cov mutagens yog genotoxic, tab sis tsis yog tag nrho cov tshuaj genotoxic yog mutagenic. Feem ntau, kev kuaj genotoxic yog ua tiav rau cov tshuaj, tshuaj siv tshuaj, thiab cov khoom siv.
Daim duab 02: Genotoxicity
Genotoxicity tuaj yeem ntsuas los ntawm vivo thiab hauv vitro kuaj rau chromosomal puas, tshwj xeeb yog los ntawm kev tshuaj xyuas chromosomal aberrations hauv metaphase hlwb. Tsis tas li ntawd, nws tseem tuaj yeem kuaj xyuas cov noob hloov pauv. Micronucleus test thiab comet assay yog ob qho kev kuaj genotoxicity. Ames kuaj feem ntau yog ua kom pom cov noob hloov pauv hauv cov kab mob.
Dab tsi yog qhov zoo sib xws ntawm Cytotoxicity thiab Genotoxicity?
- Ob leeg cytotoxicity thiab genotoxicity yog ob lub peev xwm ntawm cov tshuaj tua kab mob los ua kom puas cell lossis genetic khoom.
- Tus neeg sawv cev cytotoxic tej zaum yuav yog genotoxic (ua rau DNA puas).
Qhov txawv ntawm Cytotoxicity thiab Genotoxicity yog dab tsi?
Cytotoxicity yog lub peev xwm ua rau muaj kev puas tsuaj rau cov hlwb nyob, thaum genotoxicity yog lub peev xwm los txhawb kev hloov pauv hauv cov qauv lossis cov noob ntawm cov tshuaj lom neeg nrog DNA thiab / lossis cov hom phiaj tsis yog DNA. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm cytotoxicity thiab genotoxicity. Cytotoxic cov neeg ua haujlwm tuaj yeem ua rau cov hlwb raug mob necrosis lossis apoptosis thaum cov tshuaj genotoxic tuaj yeem hloov cov qauv, nucleotide sib lawv liag lossis tus lej ntawm cov noob hauv ib lub cell.
Summary – Cytotoxicity vs Genotoxicity
Cytotoxicity yog hais txog lub peev xwm ntawm cov tshuaj tua kab mob los ua kom puas hlwb lossis ua kom puas hlwb. Genotoxicity yog hais txog lub peev xwm ntawm cov tshuaj tua kab mob los ua kom puas cov ntaub ntawv caj ces (genome) hauv ib lub xovtooj ntawm tes. Tsis yog txhua yam tshuaj cytotoxic yog genotoxic. Txawm li cas los xij, genotoxicity tuaj yeem ua rau cytotoxicity. Yog li, qhov no qhia txog qhov sib txawv ntawm cytotoxicity thiab genotoxicity.
