Qhov sib txawv tseem ceeb ntawm CDS thiab cDNA yog CDS lossis coding sequence yog ib feem ntawm daim ntawv teev cov ntaub ntawv uas tau muab txhais ua cov protein thaum cDNA ib ntus yog DNA ib ntus tau los ntawm mRNA los ntawm kev hloov pauv rov qab.
Ib noob yog ib qho nucleotide ib ntus uas codes rau cov protein. Nws muaj cov cheeb tsam sib txawv xws li cheeb tsam txhawb nqa, qhov chaw pib pib, exons, pib codon, introns thiab nres codon. Yog li ntawd, ib tug noob muaj ob qho tib si coding thiab noncoding sequences. Coding sequences lossis CDS yog hais txog exons thiab ob codons, uas yog pib codon thiab nres codon. Nws yog cov kab ke uas tau muab txhais ua cov protein. Hauv qhov sib piv, cDNA yog DNA ib ntus muab los ntawm mRNA los ntawm kev hloov pauv rov qab.
CDS yog dab tsi?
CDS lossis coding sequence yog ib feem ntawm cov noob uas tau muab txhais ua cov protein. Nws suav nrog exons thiab ob codons li AUG codon thiab nres codon. Tsis zoo li cDNA, CDS tsis muaj ob thaj chaw tsis tau txhais: 5' UTR thiab 3 UTR. Ntxiv mus, introns tsis suav nrog hauv CDS.
Daim duab 01: Coding Sequence
Thaum muab piv rau tag nrho cov genome thiab ib tus neeg, coding sequences yog ib feem me me. Coding sequence muaj qhov tsim nyog nucleotide sequence los ua cov amino acid sequence ntawm cov protein.
cDNA yog dab tsi?
cDNA lossis ntxiv DNA yog DNA ib ntus tsim los ntawm mRNA ib ntus. Nws yog synthesized los ntawm tus txheej txheem hu ua reverse transcription. Lub enzyme thim rov qab transcriptase catalyzes cDNA synthesis, thiab mRNA sib lawv liag ua tus qauv rau cDNA synthesis. Yeej, mRNA ntawm eukaryotic hlwb tuaj yeem muab rho tawm thiab ua kom huv si thiaj li ua rau cDNA. Tom qab tsim cDNA los ntawm mRNA, lawv tuaj yeem raug cloned rau hauv cov kab mob ntawm tes txhawm rau ua cov tsev qiv ntawv cDNA. cDNA cov tsev qiv ntawv yog ib qho tseem ceeb rau kev soj ntsuam ntawm cheeb tsam coding, gene functions thiab qhia cov noob, thiab lwm yam.
Daim duab 02: cDNA
Tsis zoo li coding sequence, cDNA muaj ob UTRs, uas yog 3' UTR thiab 5' UTR. Zoo ib yam li coding sequence, cDNA tsis muaj introns.
Dab tsi yog qhov zoo sib xws ntawm CDS thiab cDNA?
- CDS thiab cDNA yog nucleic acids.
- Lawv yog tsim los ntawm deoxyribonucleotides.
- Ob CDS thiab CDNA tsis muaj introns, uas yog cov cheeb tsam uas tsis yog code.
- Ob leeg muaj cov cai caj ces lossis cov ntaub ntawv los tsim cov protein.
Qhov txawv ntawm CDS thiab cDNA yog dab tsi?
CDS lossis coding sequence yog ib feem ntawm cov noob uas tau muab txhais ua cov protein. Ntawm qhov tod tes, cDNA ib ntus yog DNA ib ntus muab los ntawm mRNA los ntawm kev hloov pauv rov qab. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm CDS thiab cDNA. Dhau li ntawd, CDS muaj exons thiab ob codons, uas yog pib codon thiab nres codon. Hauv qhov sib piv, cDNA muaj tag nrho mRNA ib ntus thiab ob UTRs.
Ntxiv mus, cDNA synthesis yog ua tiav los ntawm mRNA los ntawm kev hloov pauv rov qab thaum CDS pom hauv genomic DNA.
Hauv qab infographic qhia ntau qhov sib piv kom pom qhov txawv ntawm CDS thiab cDNA.
Summary – CDS vs cDNA
Coding sequence thiab cDNA yog ob hom nucleotide sequences. Coding sequence nyob rau hauv ib lub noob thaum cDNA yog synthesized artificially. Coding sequence muaj exons thiab ob codons thaum cDNA muaj mRNA sequence thiab ob UTRs. Ob leeg CDS thiab cDNA tsuas muaj cov nucleotide sequences uas tau txhais ua cov protein. Lawv tsis muaj introns. Tsis zoo li CDS, cDNA synthesis txheej txheem yuav tsum rov qab transcriptase enzyme. Tsis tas li ntawd, cDNA tuaj yeem hloov mus rau cDNA cov tsev qiv ntawv. Yog li, qhov no qhia txog qhov sib txawv ntawm CDS thiab cDNA.
