Qhov sib txawv tseem ceeb ntawm CLIA thiab ECLIA yog CLIA siv cov txheej txheem tshuaj los tsim cov chemiluminescence thaum ECLIA siv cov txheej txheem electrochemical los tsim cov teeb liab chemiluminescence hauv cov txheej txheem immunoassay.
Immunoassays tau siv dav hauv kev txheeb xyuas thiab ntsuas cov protein uas cuam tshuam nrog cov kab mob lossis kab mob. Yog li ntawd, lawv feem ntau vam khom lub tswv yim ntawm cov tshuaj tiv thaiv-antigen khi. Muaj ntau ntau hom immunoassays, thiab chemiluminescent immunoassays ib yam li cov txheej txheem kev loj hlob. Ob leeg CLIA thiab ECLIA siv cov ntsuas ntsuas chemiluminescent.
CLIA yog dab tsi?
Chemiluminescent Immunoassay (CLIA) yog ib hom tshuaj tiv thaiv kab mob uas siv lub luminescent molecule rau kev kuaj pom. Lub luminescence tawm los ntawm cov molecule raug kuaj pom ntawm lub spectrophotometer, feem ntau ntawm lub wavelength ntawm 300 - 800 nm. Cov chemiluminescence ua rau excitation ntawm atoms, thiab cov txheej txheem qhia exergonic tshuaj tiv thaiv los ua lub zog tsim nyog tshaj plaws los tsim excitation.
Cov txheej txheem CLIA tshwm sim hauv ob txoj hauv kev tseem ceeb raws li cov txheej txheem CLIA ncaj qha thiab cov txheej txheem CLIA tsis ncaj. Cov txheej txheem CLIA ncaj qha siv cov cim luminophore ncaj qha uas raug khi rau lub hom phiaj, thaum txoj kev tsis ncaj CLIA siv cov cim enzymatic. Cov cim luminophore ncaj qha suav nrog acidum thiab ruthenium esters thaum cov cim qhia tsis ncaj muaj xws li alkaline phosphatase nrog adamantyl 1, 2-dioxetane aryl phosphate (AMPPD) substrate thiab horseradish peroxidase nrog luminol lossis nws cov derivatives li substrate.
Daim duab 01: Chemiluminescence
Qhov txiaj ntsig tseem ceeb ntawm CLIA yog qhov dav dav ntawm kev ua haujlwm, kev siv zog siab hauv cov teeb liab xa tawm, tsis muaj kev cuam tshuam yav dhau los, qhov tshwj xeeb, nrawm, ruaj khov thiab sib haum rau cov txheej txheem ntsuas sib txawv. Nyob rau hauv sib piv, lawv cov nqi siab, thiab cov kev txwv ntawm kev soj ntsuam antigen thiab kuaj panels yog qhov tsis zoo ntawm CLIA.
ECLIA yog dab tsi?
Electrochemiluminescence Immunoassay (ELCIA) yog cov txheej txheem tshiab ntawm kev txhim kho immunoassay uas siv lub tswv yim ntawm electrochemiluminescence. Nyob rau hauv lub tswvyim ntawm electroluminescence, cov intermediates tsim electrochemically. Cov hluav taws xob tsim hluav taws xob nruab nrab no ces mus txog lub xeev zoo siab thiab ua rau lub teeb ci. Lub wavelength nyob rau hauv uas lub teeb yog emissions sib haum mus rau lub zog sib txawv. Chemiluminescence yog, yog li, tsim tawm vim muaj ib lossis ntau dua reactants raug tsim electrochemically ntawm lub electrode.
ECLIA yog ib daim ntawv thov kev tshuaj ntsuam muaj txiaj ntsig nrog kev nkag siab zoo thiab tshwj xeeb. Nws yog tsuas yog siv los txheeb xyuas cov proteins uas cuam tshuam nrog cov kab mob sib txawv thiab kev kho mob. Nws cov txiaj ntsig tseem ceeb suav nrog qhov dav dav dav dav, ntau yam, spatial thiab lub cev tswj hwm los ntawm kev siv cov peev txheej electrode, siab rhiab heev txog picomolar ntau yam. Txawm li cas los xij, qhov tseeb tias nws xav tau cov kws tshaj lij tuav thiab tus nqi siab cuam tshuam yog nws qhov tsis zoo.
Qhov Zoo sib xws ntawm CLIA thiab ECLIA yog dab tsi?
- Ob txoj kev vam khom rau lub tswv yim ntawm antigen-antibody khi.
- Ntxiv mus, ob qho tib si yog siv rau kev kuaj kab mob raws li kev ntsuas cov protein.
- Ob leeg yog rhiab thiab cov khoom siv tshwj xeeb.
- Lawv siv lub tswv yim ntawm chemiluminescence rau kev kuaj pom.
- Lawv tuaj yeem ua haujlwm tau.
- Ob leeg yuav tsum muaj spectrophotometer rau kev kuaj pom.
- Ob leeg muaj ntau qhov sib txawv.
- Nyob yog cov txheej txheem kim.
Qhov txawv ntawm CLIA thiab ECLIA yog dab tsi?
CLIA yog cov txheej txheem ntawm immunoassay uas siv txoj kev xav ntawm chemiluminescence, thaum ECLIA yog cov txheej txheem ntawm immunoassay uas siv txoj kev xav ntawm electrochemistry ua ke nrog chemiluminescence. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm CLIA thiab ECLIA. Yog li ntawd, CLIA siv tshuaj lom neeg cov tshuaj tiv thaiv uas ua rau kev txhim kho cov teeb liab chemiluminescent, thaum ECLIA siv cov tshuaj electrochemical uas ua rau kev txhim kho cov teeb liab chemiluminescent.
Cov duab hauv qab no ntawm qhov sib txawv ntawm CLIA thiab ECLIA qhia ntau qhov sib piv ntawm ob txoj kev.
Summary – CLIA vs ECLIA
CLIA thiab ECLIA yog ob txoj kev uas ua lub luag haujlwm tseem ceeb hauv kev kuaj kab mob. Lawv yog cov txheej txheem ntawm immunodiagnostics raws li lub tswv yim ntawm antibody-antigen binding. Qhov sib txawv tseem ceeb ntawm CLIA thiab ECLIA yog txoj kev tsim cov chemiluminescence. Thaum CLIA siv tshuaj lom neeg cov tshuaj tiv thaiv los tsim chemiluminescence tom qab antibody-antigen binding, ECLIA siv cov tshuaj electrochemical los tsim chemiluminescence. Txawm li cas los xij, ob qho tib si cov txheej txheem yog nrawm thiab tshwj xeeb.
