Qhov txawv ntawm Monoisotopic Mass thiab Nruab Nrab Mass

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Qhov txawv ntawm Monoisotopic Mass thiab Nruab Nrab Mass
Qhov txawv ntawm Monoisotopic Mass thiab Nruab Nrab Mass

Video: Qhov txawv ntawm Monoisotopic Mass thiab Nruab Nrab Mass

Video: Qhov txawv ntawm Monoisotopic Mass thiab Nruab Nrab Mass
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Qhov sib txawv tseem ceeb ntawm monoisotopic huab hwm coj thiab qhov nruab nrab huab hwm coj yog tias cov huab hwm coj monoisotopic suav nrog ib qho isotope, qhov nruab nrab huab hwm coj suav nrog suav tag nrho cov isotopes ntau ntawm ib qho tshuaj lom neeg.

Monoisotopic huab hwm coj thiab nruab nrab huab hwm coj yog qhov tseem ceeb hauv cov huab hwm coj spectrometry. Cov txiaj ntsig no cuam tshuam nrog cov atom ntawm cov tshuaj lom neeg.

Monoisotopic Mass yog dab tsi?

Monoisotopic huab hwm coj yog qhov loj ntawm ib qho atom ntawm ib qho isotope. Nws yog ib qho ntawm ob peb hom molecular masses uas peb siv nyob rau hauv qhov loj spectrometric tsom xam. Feem ntau, lo lus no yog siv rau cov khoom siv tshuaj muaj ib qho isotope ruaj khov uas txiav txim siab qhov nruab nrab atomic mass.

Qhov txawv tseem ceeb - Monoisotopic Mass vs Average Mass
Qhov txawv tseem ceeb - Monoisotopic Mass vs Average Mass

Daim duab 01: Cov Kws Tshuaj Ntsuam Xyuas Pawg

Ntawm no, qhov nruab nrab atomic mass yog sib npaug rau monoisotopic loj ntawm cov tshuaj lom neeg. Piv txwv li, qhov tseeb ntawm ib lub molecule los yog ib qho ion tuaj yeem suav nrog siv qhov loj ntawm cov isotopes ntau tshaj plaws uas ua rau cov molecule lossis cov ion.

Qhov Nruab Nrab Mass yog dab tsi?

Lub sij hawm nruab nrab loj yog siv los qhia qhov loj ntawm atoms. Yog li, lo lus tiag tiag yog "nruab nrab atomic mass". Nws yog qhov loj ntawm ib qho atom ntawm ib qho tshuaj lom neeg los ntawm kev txiav txim siab tag nrho cov isotopes ntawm lub caij ntawd. Ntawm no, tus nqi loj nyob ntawm qhov ntau ntawm cov tshuaj lom neeg.

Qhov sib txawv ntawm Monoisotopic Mass thiab nruab nrab pawg
Qhov sib txawv ntawm Monoisotopic Mass thiab nruab nrab pawg

Daim duab 02: Atomic Mass ntawm cov tshuaj sib txawv

Peb tuaj yeem siv ob kauj ruam tseem ceeb los xam qhov nruab nrab atomic loj ntawm cov khoom siv tshuaj. Cov kauj ruam no yog raws li nram no:

  1. Ntau qhov atomic mass ntawm txhua isotope los ntawm cov khoom noj muaj txiaj ntsig (siv qhov kev nplua nuj raws li feem pua) cais.
  2. Ntxiv cov txiaj ntsig tau ua ke kom tau txais qhov nruab nrab atomic mass.

Piv txwv li, tus nqi ntawm qhov nruab nrab atomic mass ntawm carbon yog 12.02. Carbon muaj ob lub isotopes ntau: carbon-12 thiab carbon-13. Cov isotopes no muaj ntau feem pua 98% thiab 2, feem. Siv cov txiaj ntsig no, peb tuaj yeem txiav txim siab qhov nruab nrab atomic loj ntawm carbon los ntawm kev suav. Ntawm no, peb yuav tsum muab cov atomic masses ntawm txhua isotope nrog tus nqi nplua nuj. Tom qab ntawd, peb yuav tsum coj qhov kev nplua nuj raws li ob tus lej tso rau tus nqi, tsis yog qhov feem pua. Tom ntej no, peb tuaj yeem ntxiv cov txiaj ntsig tau.

For carbon-12: 0.98 x 12=11.76

For carbon-13: 0.02 x 13=0.26

Nruab nrab atomic mass ntawm carbon=11.76 + 0.26=12.02.

Dab tsi yog qhov txawv ntawm Monoisotopic pawg thiab nruab nrab pawg?

Monoisotopic huab hwm coj thiab nruab nrab huab hwm coj yog qhov tseem ceeb hauv cov huab hwm coj spectrometry. Cov txiaj ntsig no cuam tshuam nrog cov atom ntawm cov tshuaj lom neeg tshwj xeeb. Qhov sib txawv tseem ceeb ntawm monoisotopic huab hwm coj thiab qhov nruab nrab huab hwm coj yog tias cov huab hwm coj monoisotopic suav nrog ib qho isotope, qhov nruab nrab huab hwm coj suav nrog suav tag nrho cov isotopes ntau ntawm ib qho tshuaj lom neeg.

Qhov sib txawv ntawm Monoisotopic pawg thiab nruab nrab pawg hauv daim ntawv Tabular
Qhov sib txawv ntawm Monoisotopic pawg thiab nruab nrab pawg hauv daim ntawv Tabular

Summary – Monoisotopic Mass vs Average Mass

Monoisotopic huab hwm coj thiab nruab nrab huab hwm coj yog qhov tseem ceeb hauv cov huab hwm coj spectrometry. Cov txiaj ntsig no cuam tshuam nrog cov atom ntawm cov tshuaj lom neeg tshwj xeeb. Qhov sib txawv tseem ceeb ntawm monoisotopic huab hwm coj thiab qhov nruab nrab huab hwm coj yog tias cov huab hwm coj monoisotopic suav nrog ib qho isotope, qhov nruab nrab huab hwm coj suav nrog suav tag nrho cov isotopes ntau ntawm ib qho tshuaj lom neeg.

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