Qhov sib txawv tseem ceeb ntawm chromosome banding thiab chromosome painting yog chromosome banding yog cov txheej txheem staining uas qhia cov cheeb tsam ntawm chromosomes nyob rau hauv qhov txawv qhov tsaus thiab lub teeb bands, tab sis chromosome painting yog ib tug hybridization txheej txheem nyob rau hauv uas tej cheeb tsam los yog ib feem ntawm chromosomes yog. pleev xim rau nrog cov kab ke tshwj xeeb fluorescently sau npe probes.
Nyob rau hauv cytogenetics, nws yog ib qho tsim nyog los tsim cov karyotype pom ntawm cov kab mob txhawm rau txheeb xyuas cov chromosomes thiab lawv qhov aberrations. Chromosome banding thiab chromosome painting yog ob txoj kev cytogenetic uas pab kom pom cov chromosomes. Ob txoj kev no muaj txiaj ntsig los txheeb xyuas cov kab mob caj ces.
Chromosome Banding yog dab tsi?
Chromosome banding yog txheej txheem staining uas qhia cov teeb ci thiab tsaus nti lossis thaj chaw raws qhov ntev ntawm chromosome. Ib pawg hais txog ib feem ntawm cov chromosome uas paub meej qhov txawv ntawm nws cov ntu uas nyob ib sab los ntawm qhov tshwm sim tsaus dua lossis sib dua. Txhawm rau tsim cov duab tsaus thiab lub teeb, nws yog qhov yuav tsum tau stain cov chromosomes siv cov xim zoo nkauj xws li fluorescent dye quinacrine lossis Giemsa stain.
Muaj ntau hom kev siv chromosomal banding. Ntawm lawv, Q-banding, Rov qab (R) banding thiab G-banding yog cov txheej txheem dav dav. G banding txheej txheem siv cov stain hu ua Giemsa stain, thiab nws stains AT-nplua nuj cheeb tsam ntawm heterochromatic cheeb tsam nyob rau hauv darkly stained bands thiab GC nplua nuj euchromatic cheeb tsam nyob rau hauv lub lightly stained bands. R banding yog qhov thim rov qab ntawm G banding, thiab nws stains GC cov cheeb tsam nplua nuj nyob rau hauv qhov tsaus ntuj bands thiab AT-nplua nuj cheeb tsam nyob rau hauv lub teeb bands. Q banding, ntawm qhov tod tes, siv fluorescent dye quinacrine thiab nws stains chromosomes hauv cov fluorescence daj ntawm qhov sib txawv. C banding txheej txheem yog lwm cov txheej txheem banding uas stains centromere cheeb tsam. Ntxiv mus, T banding txheej txheem pom cov cheeb tsam telomere.
Daim duab 01: Chromosome Banding
Cov qauv banding yog tshwj xeeb rau cov kab mob. Yog li, cov qauv sib txawv tshwj xeeb no yog qhov tseem ceeb hauv kev txheeb xyuas cov chromosomes thiab kuaj cov chromosomal aberrations (chromosome breakage, poob, duplication, translocation los yog inverted segments).
Chromosome Painting yog dab tsi?
Chromosome painting yog txheej txheem uas cov cheeb tsam tshwj xeeb lossis ntu ntawm chromosomes tau pleev xim nrog cov kab ke tshwj xeeb uas tau sau npe fluorescently. Yog li ntawd, chromosomal painting yog ib txwm ua ke nrog rau hauv situ FISH txheej txheem. Nws yog lig raws li cov molecular hybridization nrog ib ntus-specific probes ntawm chromosomes. Li no, nws xav tau tshwj xeeb cov kev sim kom hybridize lub hom phiaj chromosomes lossis thaj tsam chromosome.
Daim duab 02: Chromosomal Painting
Ntxiv mus, cov txheej txheem no pib xav tau qhov denaturation ntawm lub hom phiaj chromosomal DNA uas nyob rau hauv metaphase. Qhov thib ob, cov kauj ruam hybridization yuav siv qhov chaw nrog kev sojntsuam. Thaum cov kws tshawb nrhiav pom lawv cov kab ke sib txuas ntxiv, lawv sib xyaw nrog cov cheeb tsam tshwj xeeb ntawm chromosome. Peb tuaj yeem yooj yim soj ntsuam cov chaw hybridized siv autoradiography lossis immunofluorescence. Kev npaj ntawm probes, denaturation, hybridization thiab visualization yog plaub kauj ruam tseem ceeb koom nrog hauv chromosomal painting.
Kev hais txog kev siv, chromosomal painting yog pab tau thaum txheeb xyuas chromosomal rearrangements, breakpoints thiab nyob rau hauv kev txiav txim siab ntawm extrachromosomal cov ntaub ntawv. Tsis tas li ntawd, nws yog ib qho cuab yeej muaj zog hauv kev txheeb xyuas qhov tseeb ntawm cov noob sib txawv ntawm chromosomes ntawm qib microscopic. Tsis tas li ntawd, chromosomal painting yog pab tau rau kev txheeb xyuas cov noob rau cov cim xav tau hauv chromosomes.
Dab tsi yog qhov zoo sib xws ntawm Chromosome Banding thiab Chromosome Painting?
- Chromosome banding thiab chromosome painting yog ob txoj kev siv hauv kev tshuaj xyuas cytogenetic.
- Ob txoj kev pom cov chromosomes feem ntau hauv metaphase.
- Cov txheej txheem no tuaj yeem txheeb xyuas cov chromosomes ib txwm muaj thiab aberrations.
- Ntxiv mus, cov tswv yim no muaj txiaj ntsig zoo hauv kev txheeb xyuas cov kab mob caj ces.
Qhov txawv ntawm Chromosome Banding thiab Chromosome Painting yog dab tsi?
Chromosome banding yog txheej txheem staining uas qhia txog cov chromosomes hauv qhov tsaus thiab lub teeb, uas yog qhov txawv. Meanwhile, chromosome painting yog ib tug hybridization txheej txheem uas pleev xim rau tej cheeb tsam ntawm chromosomes nrog sequence-specific probes uas yog fluorescently labelled. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm chromosome banding thiab chromosome painting. Tsis tas li ntawd, chromosome banding tsim alternating tsaus thiab lub teeb band raws li qhov ntev ntawm chromosome, thaum chromosome painting tsim fluorescently sau cov cheeb tsam ntawm chromosomes.
Ntxiv mus, chromosome banding nyob ntawm lub peev xwm ntawm chromosomal DNA kom stain nrog cov xim, thaum chromosome painting nyob ntawm cov molecular hybridization nrog sequence-specific probes ntawm chromosomes. Qhov no yog qhov sib txawv tseem ceeb ntawm chromosome banding thiab chromosome painting. Dhau li ntawm cov no, tsis zoo li chromosome banding, chromosome painting txheej txheem yuav tsum tau ib ntus tshwj xeeb fluorescently labeled probes.
Summary – Chromosome Banding vs Chromosome Painting
Chromosome banding thiab chromosome painting yog ob txoj kev siv los pom cov chromosomes, feem ntau hauv metaphase. Ob txoj kev pab txheeb xyuas cov lej thiab cov qauv chromosomal aberrations thiab kab mob caj ces. Chromosome banding yog cov txheej txheem staining uas qhia cov cheeb tsam tshwj xeeb ntawm chromosomes hauv qhov tsaus thiab lub teeb, uas yog qhov txawv. Lub caij no, chromosomal painting yog ib hom hybridization txheej txheem uas pom cov cheeb tsam tshwj xeeb ntawm chromosomes vim yog hybridization nrog fluorescently sau npe ib ntus tshwj xeeb probes. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm chromosome banding thiab chromosome painting.
