Qhov Tseem Ceeb - Ntshav Agar vs MacConkey Agar
Microbes xav tau cov as-ham txaus thiab tsim nyog rau kev loj hlob rau lawv txoj kev loj hlob zoo. Raws li qhov yuav tsum tau ua, kab lis kev cai xov xwm tuaj yeem tsim thiab npaj los ntawm kev tsom mus rau hom kab mob tshwj xeeb lossis ib pawg tshwj xeeb. Ib kab lis kev cai nruab nrab yog txhais tias yog ib qho khoom siv los yog kua npaj tsim los txhawb kev loj hlob ntawm cov kab mob. Muaj ntau hom kab lis kev cai xov xwm muaj nyob rau cais thiab txheeb xyuas cov kab mob hauv cov chaw kuaj mob. Cov xov xwm xaiv, xov xwm sib txawv, cov khoom noj khoom haus thiab cov khoom noj muaj txiaj ntsig yog ntau pawg. Ntshav agar thiab MacConkey agar yog ob hom xov xwm sib txawv uas yog nyob rau hauv qeb ntawm cov xov xwm sib txawv. Qhov sib txawv tseem ceeb ntawm cov ntshav agar thiab MacConkey agar yog cov ntshav agar yog ib qho kev sib txawv sib txawv nruab nrab siv los cais cov kab mob yoov tshaj cum thiab kuaj xyuas lawv cov kev ua haujlwm hemolytic whereas MacConkey agar yog ib qho kev xaiv thiab sib txawv nruab nrab siv los sib txawv ntawm cov kab mob uas tsis yog-fastidious gram-negative kab mob los ntawm gram. - cov kab mob zoo.
Ntshav Agar yog dab tsi?
Ntshav agar yog ib qho khoom noj khoom haus sib txawv uas txhawb kev loj hlob ntawm ntau hom kab mob yoov tshaj cum. Nws raug suav hais tias yog ib qho tsis xaiv nruab nrab vim nws tso cai rau kev loj hlob ntawm cov kab mob feem ntau. Ntshav yog enriched nrog cov as-ham. Li no, ntshav agar txhawb kev loj hlob ntawm cov kab mob yoov tshaj cum uas tsis yooj yim rau kev loj hlob hauv kab lis kev cai xov xwm. Ntshav agar muaj cov khoom sib txawv vim nws cov khoom hemolysis. Qhov nruab nrab no tuaj yeem txheeb xyuas qhov kev puas tsuaj ntawm erythrocytes los ntawm cov kab mob loj hlob. Kev ua tiav ntawm cov qe ntshav liab (beta (β) hemolysis) tau lees paub los ntawm thaj chaw ntshiab tsim nyob ib puag ncig cov kab mob kab mob. Kev puas tsuaj ib nrab ntawm RBCs (alpha (α) hemolysis) tuaj yeem lees paub los ntawm kev tsim cov xim ntsuab xim av ntawm agar nruab nrab. Gamma (γ) hemolysis raug lees paub thaum cov kab mob loj hlob tsis hloov nruab nrab thiab tsis ua rau cov qe ntshav liab.
Cov Ntshav Agar Nruab Nrab - Ib Liter
| Cov khoom xyaw | Nyiaj |
| YPeptone | 5 g |
| Neeg Extract/Nyeast Extract | 3 g |
| Sodium chloride | 5 g |
| Yaj cov ntshav | 50ml |
| Agar | 15g |
| pH | 7.2 - 7.6 |
Daim duab 01: Ntshav Agar Phaj
Macconkey Agar yog dab tsi?
MacConkey agar yog ib qho kev xaiv thiab sib txawv nruab nrab tsim los cais thiab txheeb xyuas cov kab mob gram-tsis zoo thaum tiv thaiv kev loj hlob ntawm cov kab mob gram-zoo. MacConkey agar nruab nrab muaj ob qho tib si xaiv thiab sib txawv. Nws txhawb kev loj hlob ntawm cov kab mob gram-tsis zoo. Nws kuj muab qhov sib txawv ntawm cov kab mob gram-tsis zoo los ntawm kev muab cov yam ntxwv loj hlob sib txawv rau qhov nruab nrab. Selectivity yog muab los ntawm ob yam hauv nruab nrab: bile ntsev thiab crystal violet. Kev sib txawv yog muab los ntawm lwm ob lub npe hu ua lactose thiab nruab nrab liab qhia. Vim qhov kev ua ob zaug no, MacConkey agar nruab nrab muaj ntau yam kev siv hauv kev kho mob thiab ib puag ncig.
Daim duab 02: MacConkey Agar
Bile ntsev thiab siv lead ua violet dye ua inhibitors rau feem ntau cov kab mob gram-zoo. Yog li, qhov nruab nrab no tso cai rau cov kab mob gram-negative nkaus xwb kom loj hlob thiab pom cov cheeb tsam pom. Lactose sib txawv lactose fermenting kab mob los ntawm cov tsis-fermenters. Thaum lactose fermenters (lactose zoo) siv lactose, lawv tso cov kua qaub rau hauv nruab nrab. Nws txo qis pH ntawm agar nruab nrab thiab ua rau cov xim liab lossis xim liab. Cov kab mob uas tsis yog fermenter (lactose negative) cov kab mob tshwm sim dawb los yog tsis muaj xim nyob rau hauv nruab nrab.
Composition of MacConkey Agar Medium - Ib Litre
| Cov khoom xyaw | Nyiaj |
| YPeptone (Pancreatic digest ntawm gelatin) | 17g |
| Proteose peptone (nqaij thiab casein) | 3 g |
| YLactose monohydrate | 10g |
| bile ntsev | 1.5 g |
| Sodium chloride | 5 g |
| Neutral red | 0.03 g |
| YCrystal Violet | 0.001 g |
| Agar | 13.5g |
Dab tsi yog qhov txawv ntawm Ntshav Agar thiab Macconkey Agar?
Blood Agar vs Macconkey Agar |
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| Ntshav agar yog kab lis kev cai nruab nrab tsim los loj hlob ntau yam kab mob yoov tshaj cum thiab kuaj xyuas lawv cov dej num hemolytic. | MacConkey agar yog kab lis kev cai nruab nrab tsim los cais cov kab mob gram-tsis zoo thiab sib txawv lactose fermenters los ntawm cov tsis-fermenters. |
| Ntshav agar muaj peptone, nqaij nyug extract lossis poov xab extract, sodium chloride, agar, yaj cov ntshav thiab dej. | Y MacConkey agar muaj peptone, proteose peptone, lactose, bile ntsev, crystal violet, sodium chloride, nruab nrab liab, agar, thiab dej. |
| khoom | |
| ntshav agar qhia tau tias muaj txiaj ntsig zoo thiab sib txawv. | MacConkey agar qhia cov khoom xaiv thiab qhov sib txawv. |
| ntshav agar yog siv los loj hlob cov kab mob fastidious thiab sib txawv hemolytic kev ua ntawm cov kab mob. | Qhov no yog siv los cais cov kab mob gram-tsis zoo, sib txawv cov kab mob lactose fermenting thiab cov kab mob uas tsis yog fermenting thiab cais cov coliform thiab cov kab mob plab hnyuv hauv dej, cov khoom noj mis thiab cov khoom siv lom neeg. |
Summary – Ntshav Agar vs MacConkey Agar
Ntshav agar thiab MacConkey agar yog ob qhov sib txawv sib txawv siv los cog cov kab mob. MacConkey agar yog siv los xaiv cov kab mob gram-tsis zoo thiab sib txawv cov kab mob lactose fermenting los ntawm cov kab mob uas tsis yog fermenting. Ntshav agar yog enriched nrog ntshav as-ham. Li no, nws yog siv los loj hlob cov kab mob fastidious thiab sib txawv raws li lawv cov qauv hemolytic. Qhov no yog qhov txawv ntawm cov ntshav agar thiab MacConkey agar.
Download PDF Version of Blood Agar vs MacConkey Agar
Koj tuaj yeem rub tawm PDF version ntawm kab lus no thiab siv nws rau offline hom phiaj raws li cov ntawv sau tseg. Thov download PDF version ntawm no Qhov txawv Ntawm Bood Agar thiab Macconkey Agar.
