Qhov Tseem Ceeb Qhov txawv - Trypsin vs Chymotrypsin
Protein digestion yog ib qho tseem ceeb heev nyob rau hauv tag nrho cov digestion txheej txheem nyob rau hauv cov kab mob. Cov proteins nyuaj yog zom rau hauv nws cov monomers ntawm cov amino acids thiab tau nqus los ntawm cov hnyuv me. Proteins yog qhov tseem ceeb vim lawv ua haujlwm tseem ceeb thiab lub luag haujlwm hauv lub cev. Protein digestion tshwm sim los ntawm cov protein digesting enzymes uas muaj xws li trypsin, chymotrypsin, peptidases, thiab proteases. Trypsin yog ib qho protein-digesting enzyme uas cleaves peptide daim ntawv cog lus ntawm cov amino acids uas muaj xws li lysine thiab arginine. Chymotrypsin kuj yog ib qho protein-digesting enzyme uas cleaves peptide daim ntawv cog lus ntawm aromatic amino acids xws li phenylalanine, tryptophan, thiab tyrosine. Qhov sib txawv tseem ceeb ntawm trypsin thiab chymotrypsin yog txoj hauj lwm ntawm cov amino acid uas nws cleaves hauv cov protein. Trypsin cleaves ntawm qhov yooj yim amino acid txoj hauj lwm whereas chymotrypsin cleaves ntawm aromatic amino acid txoj hauj lwm.
Trypsin yog dab tsi?
Trypsin yog 23.3 kDa protein uas belongs rau tsev neeg ntawm serine proteases thiab nws cov substrates tseem ceeb yog cov amino acids yooj yim. Cov amino acids tseem ceeb no suav nrog arginine thiab lysine. Trypsin tau tshawb pom hauv 1876 los ntawm Kuhne. Trypsin yog ib qho globular protein thiab muaj nyob rau hauv nws cov inactive daim ntawv uas yog trypsinogen - zymogen. Cov txheej txheem ntawm kev ua ntawm trypsin yog raws li kev ua haujlwm ntawm serine protease.
Trypsin cleaves ntawm C terminal kawg ntawm cov amino acids yooj yim. Qhov no yog cov tshuaj tiv thaiv hydrolysis thiab tshwm sim ntawm pH - 8.0 hauv cov hnyuv. Kev ua kom muaj trypsinogen tshwm sim los ntawm kev tshem tawm ntawm lub davhlau ya nyob twg hexapeptide, thiab nws ua rau daim ntawv nquag; trypsin. Active trypsin yog ob hom loj; α - trypsin thiab β-trypsin. Lawv txawv ntawm lawv cov thermal stability thiab lawv cov qauv. Qhov chaw nquag ntawm trypsin muaj Histidine (H63), Aspartic acid (D107) thiab Serine (S200).
Daim duab 01: Trypsin
Lub zog ua haujlwm ntawm trypsin yog inhibited los ntawm DFP, aprotinin, Ag+, Benzamidine, thiab EDTA. Cov ntawv thov ntawm trypsin muaj xws li dissociation ntawm cov ntaub so ntswg, trypsinization nyob rau hauv cov tsiaj cell kab lis kev cai, tryptic mapping, nyob rau hauv vitro protein kev tshawb fawb, fingerprinting thiab nyob rau hauv daim ntaub ntawv kab lis kev cai.
Chymotrypsin yog dab tsi?
Chymotrypsin muaj qhov hnyav ntawm 25.6 kDa thiab belongs rau tsev neeg serine protease, thiab nws yog endopeptidase. Chymotrypsin muaj nyob rau hauv nws daim ntawv inactive uas yog chymotrypsinogen. Chymotrypsin tau tshawb pom hauv xyoo 1900s. Chymotrypsin hydrolyzes peptide bonds ntawm aromatic amino acids. Cov tshuaj tsw qab no muaj xws li tyrosine, phenylalanine, thiab tryptophan. Cov substrates ntawm cov enzyme no feem ntau yog nyob rau hauv L-isomers thiab nkag mus rau cov amides thiab esters ntawm cov amino acids. Qhov zoo tshaj pH nyob rau hauv uas chymotrypsin ua yog 7.8 - 8.0. Muaj ob lub ntsiab lus ntawm chymotrypsin xws li chymotrypsin A thiab chymotrypsin B thiab lawv txawv me ntsis nyob rau hauv muaj cov yam ntxwv thiab proteolytic. Qhov chaw nquag ntawm chymotrypsin muaj cov catalytic triad thiab muaj xws li Histidine (H57), Aspartic acid (D102) thiab Serine (S195).
Daim duab 02: Chymotrypsin
Cov activators ntawm chymotrypsin yog Cetyltrimethylammonium bromide, Dodecyltrimethylammonium bromide, Hexadecyltrimethylammonium bromide thiab Tetrabutylammonium bromide. Cov inhibitors ntawm chymotrypsin yog peptidyl aldehydes, boronic acids, thiab coumarin derivatives. Chymotrypsin yog kev lag luam siv hauv peptide synthesis, peptide mapping thiab peptide fingerprinting.
Dab tsi yog qhov zoo sib xws ntawm Trypsin thiab Chymotrypsin?
- Ob lub enzymes yog serine proteases.
- Ob lub enzymes cleave peptide bonds.
- Ob lub enzymes ua haujlwm hauv cov hnyuv.
- Ob lub enzymes muaj nyob hauv nws daim ntawv tsis ua haujlwm li zymogens.
- Ob lub enzymes yog tsim los ntawm catalytic triad uas muaj histidine, aspartic acid, thiab serine hauv nws qhov chaw nquag.
- Ob lub enzymes tau pib pom thiab muab rho tawm los ntawm nyuj.
- Kev tsim tawm ntawm ob lub enzymes yog ua los ntawm cov txheej txheem DNA recombinant tam sim no.
- Ob lub enzymes ua rau qhov zoo tshaj pH.
- Ob lub enzymes yog siv hauv vitro hauv kev lag luam sib txawv.
Qhov txawv ntawm Trypsin thiab Chymotrypsin yog dab tsi?
Trypsin vs Chymotrypsin |
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| Trypsin yog cov protein-digesting enzyme uas yuav tshem tawm cov peptide daim ntawv cog lus ntawm cov amino acids yooj yim xws li lysine thiab arginine. | Chymotrypsin uas tseem yog cov protein-digesting enzyme cleaves peptide daim ntawv cog lus ntawm aromatic amino acids xws li phenylalanine, tryptophan, thiab tyrosine. |
| Molecular Nyhav | |
| Lub cev hnyav ntawm trypsin yog 23.3 k Da. | Qhov hnyav molecular ntawm chymotrypsin yog 25.6 k Da. |
| Cov proteins nyuaj tau zom rau hauv nws cov monomers ntawm cov amino acids thiab tau nqus los ntawm cov hnyuv me. | Aromatic amino acids substrates xws li tyrosine, tryptophan, thiab phenylalanine ua rau chymotrypsin. |
| Zymogen Form ntawm Enzyme | |
| Trypsinogen yog daim ntawv tsis muaj zog ntawm trypsin. | Chymotrypsinogen yog daim ntawv tsis muaj zog ntawm chymotrypsin. |
| Activators | |
| Lanthanides yog cov activators ntawm trypsin. | Cetyltrimethylammonium bromide, Dodecyltrimethylammonium bromide, Hexadecyltrimethylammonium bromide thiab Tetrabutylammonium bromide yog activators ntawm chymotrypsin. |
|
Inhibitors |
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| DFP, aprotinin, Ag+, Benzamidine, thiab EDTA yog inhibitors ntawm trypsin. | Peptidyl aldehydes, boronic acids, thiab coumarin derivatives yog inhibitors ntawm chymotrypsin. |
Summary – Trypsin vs Chymotrypsin
Peptidases lossis proteolytic enzymes cleave proteins ntawm hydrolysis ntawm peptide daim ntawv cog lus. Trypsin cleaves peptide daim ntawv cog lus ntawm cov amino acids yooj yim whereas chymotrypsin cleaves peptide daim ntawv cog lus ntawm aromatic amino acid residues. Ob lub enzymes yog serine peptidases thiab ua haujlwm hauv cov hnyuv me hauv ib puag ncig pH. Tam sim no, ntau cov kev tshawb fawb tau koom nrog hauv kev tsim trypsin thiab chymotrypsin siv cov tshuab DNA recombinant los ntawm kev siv cov kab mob sib txawv thiab cov kab mob fungal vim tias cov enzymes no muaj txiaj ntsig zoo. Qhov no yog qhov txawv ntawm trypsin thiab chymotrypsin.
Download the PDF Version of Trypsin vs Chymotrypsin
Koj tuaj yeem rub tawm PDF version ntawm kab lus no thiab siv nws rau offline lub hom phiaj raws li daim ntawv ceeb toom. Thov download PDF version ntawm no Qhov txawv Ntawm Trypsin thiab Chymotrypsin
