Qhov sib txawv tseem ceeb ntawm biochemical thiab kev soj ntsuam ntawm tes yog qhov kev tshuaj ntsuam xyuas biochemical yog cov hom phiaj-raws li kev soj ntsuam thaum lub xov tooj ntawm tes yog kev soj ntsuam physiology.
Kev ntsuas yog qhov tseem ceeb hauv kev tshawb fawb thiab kev tsim tshuaj. Nws yog cov txheej txheem tshawb nrhiav hauv thaj chaw ntawm chaw muag tshuaj, tshuaj hauv chaw kuaj mob, thiab molecular biology rau kev ntsuas ntau thiab kev soj ntsuam zoo ntawm qhov muaj, kom muaj nuj nqis, thiab kev ua haujlwm ntawm lub hom phiaj. Yog li ntawd, kev soj ntsuam biochemical thiab kev soj ntsuam ntawm tes yog ob hom kev soj ntsuam uas siv rau hauv kev tsim tshuaj. Kev ntsuam xyuas biochemical yog ib qho tseem ceeb hauv kev tshawb nrhiav thiab ntsuas cov roj ntsha molecules nrog rau lawv cov haujlwm. Ntawm qhov tod tes, kev soj ntsuam ntawm tes yog qhov tseem ceeb kom tau txais cov ntaub ntawv muaj txiaj ntsig lom neeg los kwv yees thiab txheeb xyuas qib ntawm cov lus teb ntawm cov kab mob rau ib yam khoom / tshuaj.
Yam tshuaj Biochemical yog dab tsi?
Kev tshuaj lom neeg lom neeg yog cov cuab yeej siv tshuaj lom neeg rau kev tshuaj xyuas biomolecules kom muaj nuj nqis lossis zoo. Yog li, lawv tau siv los txheeb xyuas thiab txheeb xyuas cov txheej txheem loj ntawm tes xws li cell apoptosis, cell signaling, thiab metabolic reactions. Nyob rau hauv lwm yam lus, nws yog ib tug ntau biochemical txheej txheem kom nkag siab txog lub hom phiaj biomolecules thaum characterizing nws. Yog li ntawd, thaum lub sij hawm tsim tshuaj, biochemists siv pua pua ntawm biochemical ntsuam xyuas los soj ntsuam biomolecules ob qho tib si quantitatively thiab zoo.
Daim duab 01: Chemoluminescence
Raws li kev tshawb pom, muaj peb hom kev tshuaj ntsuam biochemical: colorimetric (chromogenic assays), fluorometric (fluorogenic) assays, thiab luminescent assays. Hauv kev tshuaj ntsuam colorimetric, nws tuaj yeem kuaj pom qhov hloov xim pom thaum, hauv kev tshuaj ntsuam fluorometric, nws muaj peev xwm txheeb xyuas cov teeb liab emission los ntawm excitation los ntawm lub teeb ci. Thaum kawg, luminescent kev soj ntsuam kuaj pom lub teeb tawm los ntawm cov tshuaj tiv thaiv tshuaj.
Cell Based Assays yog dab tsi?
Cell based assays yog physiology-based assays uas tso cai rau kev tshawb nrhiav cov lus teb ntawm cov kab mob lom rau ib yam khoom lossis biomolecule. Yog li ntawd, kev ntsuam xyuas ntawm tes yog qhov tseem ceeb hauv kev tsim cov tshuaj. Cov kev ntsuam xyuas no yog cov txheej txheem hauv vitro uas tau ua hauv cov kab lis kev cai ntawm tes. Los ntawm kev soj ntsuam ntawm tes, kev tswj ntawm cov noob qhia, pib lossis inhibition ntawm cov txheej txheem lom neeg hauv cov lus teb rau ib yam khoom tuaj yeem kuaj pom.
Daim duab 02: Cell Raws li kev soj ntsuam
Cov kev ntsuas ntsuas los ntawm kev soj ntsuam ntawm tes suav nrog apoptosis, cell proliferation, oxidative stress, cytotoxicity, cell adhesion, migration, transformation, invasion, and immortalization. Yog li, raws li cov kev ntsuas no, muaj ntau hom kev ntsuas ntawm tes. Lawv yog cov tshuaj ntsuam xyuas cell viability, kuaj cell proliferation, cytotoxicity assays, cell senescence assays, thiab cell death assays.
Dab tsi zoo sib xws ntawm Biochemical thiab Cell Based Assays?
- Kev tshuaj xyuas biochemical thiab cell-based yog ob hom hauv vitro
- Ob hom no pab txhawm rau txheeb xyuas qhov cuam tshuam ntawm biomolecules ntawm cov kab mob lom thaum lub sijhawm tsim tshuaj.
Qhov txawv ntawm Biochemical thiab Cell Raws li kev soj ntsuam yog dab tsi?
Kev tshuaj ntsuam biochemical yog lub hom phiaj raws li kev soj ntsuam thaum lub xov tooj ntawm tes ntsuas yog physiology raws li kev soj ntsuam. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm biochemical thiab cell raws li kev soj ntsuam. Functionally, biochemical assays soj ntsuam cov kev ua ntawm bio-molecules kom muaj nuj nqis thiab zoo thaum cell raws li kev soj ntsuam xyuas cov lus teb ntawm cov kab mob tshwj xeeb rau ib yam khoom los yog tshuaj. Li no, qhov no yog qhov sib txawv ntawm biochemical thiab cell raws li kev soj ntsuam.
Hauv qab infographic qhia txog qhov sib txawv ntawm biochemical thiab cell raws li kev soj ntsuam.
Summary – Biochemical vs Cell Based Assays
Assays yog cov cuab yeej tshuaj ntsuam xyuas kom muaj nuj nqis thiab kev soj ntsuam zoo ntawm lub hom phiaj molecule / khoom nyob rau hauv cov ntsiab lus ntawm muaj, kom muaj nuj nqis thiab ua haujlwm. Yog li ntawd, lawv yog cov tseem ceeb hauv kev tsim cov tshuaj. Biochemical thiab cell raws li kev soj ntsuam yog ob qhov kev ntsuam xyuas muaj txiaj ntsig hauv kev lag luam tshuaj thiab molecular biology. Qhov sib txawv tseem ceeb ntawm biochemical thiab kev soj ntsuam ntawm tes yog hom txheej txheem thiab kev ntsuas. Qhov ntawd yog; Cov kev ntsuam xyuas biochemical yog lub hom phiaj raws li kev soj ntsuam ntawm tes yog physiology raws li.
