Qhov sib txawv tseem ceeb ntawm HPLC thiab UPLC yog tias HPLC tso cai rau kev tshuaj xyuas cov khoom uas muaj lawv qhov loj nyob ib ncig ntawm 5 micrometers whereas UPLC tso cai rau ntau qhov me me nyob ib ncig ntawm 2 micrometers.
HPLC thiab UPLC yog ob qho tib si Liquid Chromatographic cov txheej txheem uas muaj txiaj ntsig zoo hauv kev sib cais cov khoom ntawm cov khoom sib xyaw. Kom paub qhov txawv ntawm HPLC thiab UPLC, peb yuav tsum xub paub tias HPLC yog dab tsi. Qhov no yog vim UPLC yog qhov tshwj xeeb ntawm HPLC thiab peb tuaj yeem nkag siab yooj yim yog tias peb paub tias HPLC yog dab tsi.
HPLC yog dab tsi?
Lub sij hawm HPLC sawv cev rau Kev Ua Tau Zoo Ua Kua Dej Chromatography. Nws yog ib qho txheej txheem uas peb tuaj yeem siv los cais cov khoom sib txawv ntawm cov khoom sib xyaw. Tsis tas li ntawd, nws yog cov txheej txheem siv dav tshaj plaws los txheeb xyuas, ntsuas thiab cais cov khoom sib xyaw. Tsis tas li ntawd, cov txheej txheem no siv lub zog siab los thawb cov kuab tshuaj los ntawm kab. Tsis tas li ntawd, cov ntawv thov tseem ceeb ntawm HPLC yog nyob rau hauv biochemistry rau kev tsom xam ntawm cov khoom sib xyaw. Nws yog ib txoj hauv kev zoo tshaj plaws rau kev sib cais thiab txheeb xyuas cov amino acids, nucleic acids, proteins, hydrocarbons, tshuaj tua kab, carbohydrates, tshuaj tua kab mob, steroids thiab suav tsis txheeb lwm yam tshuaj inorganic.
Daim duab 01: HPLC System hauv chav kuaj
Hauv luv luv, txoj kev ua haujlwm ntawm HPLC cov txheej txheem yog raws li hauv qab no. Ua ntej, peb yuav tsum qhia tus qauv mus rau hauv cov kwj ntawm theem mobile hauv kev sib cais me me uas siv lub twj tso kua mis. Lub twj tso kua mis siab yuav tsum tau khaws cia ntawm 40 MPa. Tom qab ntawd, cov kwj ntawm lub xov tooj ntawm tes no percolates los ntawm kem ntawm HPLC. Tsis tas li ntawd, cov khoom hauv cov qauv dhau los ntawm kab. Txawm li cas los xij, lawv qhov nrawm ntawm kev txav yog txawv ntawm ib leeg. Qhov sib txawv no tshwm sim vim qhov sib txawv ntawm kev sib cuam tshuam ntawm cov qauv piv txwv thiab cov adsorbent hauv kab. Peb hu cov adsorbent no ua lub sijhawm nyob ruaj khov vim nws nyob hauv kem (tsis txav). Qhov thib ob, thaum kawg ntawm kab ntawv, peb tuaj yeem sau cov qauv uas elutes los ntawm kem. Nyob ntawd peb tuaj yeem txiav txim siab qhov sib txawv ntawm lub sijhawm khaws cia rau txhua yam hauv cov qauv.
UPLC yog dab tsi?
Lub sij hawm UPLC sawv rau Ultra High-Performance Liquid Chromatography. Nws yog qhov hloov pauv ntawm HPLC, tab sis txoj kev ua haujlwm yog tib yam. Txawm li cas los xij, tsis zoo li HPLC, txoj kev no tso cai rau cov khoom me me los soj ntsuam, xws li 2 micrometers me me.
Daim duab 02: A UPLC Device
Ntxiv mus, nws muab kev tshuaj xyuas sai dua. Hauv cov txheej txheem no, peb siv 100 MPa lub twj tso kua mis siab rau kev ntxig cov qauv rau hauv kab. Yog li ntawd, qhov kev kub siab no lees paub tias tag nrho cov qauv yog nkag mus rau hauv kem. Yog li, qhov no ua tau zoo heev piv rau HPLC.
Qhov txawv ntawm HPLC thiab UPLC yog dab tsi?
Lub sij hawm HPLC sawv cev rau Kev Ua Tau Zoo Ua Kua Dej Chromatography whereas lub sij hawm UPLC sawv cev rau Ultra High Performance Liquid Chromatography. Yog li ntawd, cov txheej txheem UPLC yog ib qho tsim tawm ntawm HPLC. Yog li, txoj kev ua haujlwm yog tib yam rau ob txoj kev. Txawm li cas los xij, UPLC muaj txiaj ntsig zoo dua piv rau HPLC vim nws siv lub twj tso kua mis siab; Lub twj tso kua mis siab rau HPLC yog 40 MPA thaum nws yog 100 MPa rau UPLC. Yog li, qhov no yog qhov sib txawv ntawm HPLC thiab UPLC. Qhov tseem ceeb tshaj plaws, qhov sib txawv tseem ceeb ntawm HPLC thiab UPLC yog tias HPLC tso cai rau kev tshuaj xyuas cov khoom uas muaj lawv qhov loj nyob ib ncig ntawm 5 micrometers whereas UPLC tso cai rau ntau qhov me me nyob ib ncig ntawm 2 micrometers.
Summary – HPLC vs UPLC
UPLC yog ib daim ntawv ntawm HPLC. Yog li, cov txheej txheem ua haujlwm yog tib yam. Tab sis, peb tuaj yeem pom qee qhov kev txhim kho hauv UPLC piv rau HPLC. Yog li, muaj qee qhov sib txawv ntawm HPLC thiab UPLC. Txawm li cas los xij, qhov sib txawv tseem ceeb ntawm HPLC thiab UPLC yog tias HPLC tso cai rau kev soj ntsuam cov khoom uas muaj lawv qhov loj nyob ib ncig ntawm 5 micrometers whereas UPLC tso cai rau ntau qhov me me nyob ib ncig ntawm 2 micrometers.
