Qhov sib txawv tseem ceeb ntawm metalloenzymes thiab hlau activated enzymes yog tias cov metalloenzymes muaj cov hlau ion ruaj khov ua cov cofactor whereas hlau ions hauv hlau qhib enzymes tsis ruaj khov.
Cov haujlwm ntawm qee cov enzymes nyob ntawm cov hlau ions vim tias cov hlau ions ua haujlwm ua cofactors. Cov enzymes no yog nyob rau hauv ob pawg loj xws li metalloenzymes thiab hlau activated enzymes. Yog li ntawd, cov enzymes no txawv ntawm ib leeg raws li qhov muaj lossis tsis muaj cov hlau ions nruj nreem. Cia peb tham ntxiv txog cov enzymes no.
Metalloenzymes yog dab tsi?
Metalloenzymes yog cov enzymes uas muaj cov hlau ion nruj nreem. Cov hlau ion no ua haujlwm sib koom ua ke covalent bonds nrog cov amino acids ntawm cov enzyme los yog nrog ib pab pawg neeg prosthetic. Tsis tas li ntawd, nws ua raws li coenzyme thiab txhawb kev ua haujlwm ntawm enzyme. Thaum xav txog qhov chaw ntawm cov hlau ion nyob rau hauv lub enzyme, nws feem ntau tshwm sim nyob rau hauv ib cheeb tsam ntawm lub enzyme nto. Yog li ntawd, ion tsis cuam tshuam kev khi ntawm lub substrate nrog rau qhov chaw nquag. Qee zaum, enzymes xav tau ntau dua ib qho hlau ion rau nws txoj haujlwm. Nyob rau hauv tsawg zaus, lawv xav tau ob yam hlau ions thiab. Cov hlau feem ntau uas koom nrog hauv qhov no yog Fe, Zn, Cu, thiab Mn. Cov metalloenzymes uas muaj cov chaw hlau uas tsis yog hlau (cov chaw tsis yog heme) yog qhov dav dav hauv qhov xwm txheej.
Daim duab 01: Enzyme Action
Piv txwv ntawm metalloenzymes:
- Amylase, thermolysin khi nrog Ca2+ ions
- Dioldehydrase, glycerol dehydratase yog khi nrog Co2+
- Cytochrome c oxidase, dopamine-b-hydroxylase muaj Cu2+
- Catalase, nitrogenase, peroxidase, succinate dehydrogenase muaj Fe2+
- Arginase, histidine-ammonia lyase, pyruvate carboxylase muaj Mn2+
Hlau Activated Enzymes yog dab tsi?
Hlau activated enzymes yog cov enzymes uas muaj kev ua haujlwm ntau ntxiv vim muaj cov hlau ions. Feem ntau, cov hlau ions no yog monovalent lossis divalent. Txawm li cas los xij, cov ions no tsis nruj nrog cov enzymes xws li hauv metalloenzymes. Cov hlau tuaj yeem qhib lub substrate, yog li koom ncaj qha nrog kev ua haujlwm ntawm enzyme. Cov enzymes no xav tau hlau ions ntau dhau. Piv txwv li: nyob ib ncig ntawm 2-10 lub sij hawm siab tshaj qhov concentration ntawm enzyme. Nws yog vim lawv tsis tuaj yeem sib txuas nrog cov hlau ion mus tas li. Txawm li cas los xij, cov enzymes no poob lawv txoj haujlwm thaum lub sijhawm ua kom huv.
Piv txwv ntawm meta activated enzymes:
- Pyruvate kinase xav tau K+
- Phosphotransferases xav tau Mg2+ los yog Mn2+
Qhov txawv ntawm Metalloenzymes thiab Hlau Activated Enzymes yog dab tsi?
Cov metalloenzymes yog cov enzymes uas muaj cov hlau ion nruj nreem. Raws li ib tug tshwj xeeb yam ntxwv, lawv muaj ib tug ruaj khov khi hlau ion raws li lub cofactor. Ntxiv mus, cov enzymes no xav tau ib lossis ob lub hlau ions khi rau ib cheeb tsam tshwj xeeb ntawm cov enzymes rau lawv cov haujlwm. Hlau activated enzymes yog cov enzymes uas muaj kev ua haujlwm ntau ntxiv vim muaj cov hlau ions uas tsis ruaj khov. Nws yog qhov sib txawv tseem ceeb ntawm metalloenzymes thiab hlau activated enzymes. Ntawd yog, tsis zoo li cov metalloenzymes, cov hlau ua haujlwm enzymes tsis muaj cov hlau ion ruaj khov li cov cofactor. Ntxiv rau qhov ntawd, cov enzymes no yuav tsum muaj qhov siab ntawm cov hlau ions nyob ib puag ncig lawv.
Summary – Metalloenzymes vs Hlau Activated Enzymes
Lub enzyme, uas cov haujlwm nyob ntawm seb muaj cov hlau ions, yog ob hom; Lawv yog, metalloenzymes thiab hlau qhib enzymes. Qhov txawv ntawm metalloenzymes thiab hlau activated enzymes yog tias cov metalloenzymes muaj ib tug ruaj khov khi hlau ion raws li cov cofactor whereas cov hlau ions nyob rau hauv hlau activated enzymes tsis ruaj khov.
