Qhov sib txawv tseem ceeb ntawm kev ntxig thiab hloov cov vectors yog qhov ntxig vector muaj peev xwm los ntxig qhov ntev ntawm cov DNA txawv teb chaws thaum hloov vector muaj peev xwm ua kom haum qhov ntev ntawm cov DNA txawv teb chaws.
Phage vectors yog cov kab mob bacteriophages siv rau cloning. Muaj ob hom phage vectors; lawv yog insertion vector thiab hloov vector. Tsis tas li ntawd, tag nrho cov phage vectors muaj cov noob tsis tseem ceeb. Txawm li cas los xij, cov noob no yuav tsum tau muab tshem tawm ntawm phages los pab txhawb DNA txawv teb chaws tshiab.
Vector Insertion yog dab tsi?
Ua ntej ntawm tag nrho, kev ntxig vector yog qhov yooj yim tshaj plaws ntawm lambda cloning vectors. Qhov tseeb, nws yog hom phage vector uas muaj qhov tshwj xeeb txwv qhov chaw qhia nyob rau hauv vector genome ntawm txoj hauj lwm ntawm kev xaiv DNA. Tsis tas li ntawd, phage DNA tseem nyob tsis muaj kev tshem tawm. Qhov no tsis yog tshem tawm ntawm phage DNA curtails qhov luaj li cas ntawm cov inserts (hloov DNA txawv teb chaws) yuav tsum tau cloned nyob rau hauv lub vector. Ntxiv mus, cov vectors no muaj txiaj ntsig zoo hauv cDNA cloning thiab qhia. GT10, GT11, thiab Zap yog cov piv txwv ntawm no vector.
Daim duab 01: Lambda Phag
Insertion vector muaj ib qhov chaw lees paub. Lub luag haujlwm tseem ceeb ntawm cov vector no yog tsim cov tsev qiv ntawv cDNA tau los ntawm eukaryotic mRNA sequences. Ntxiv mus, nws tsuas tuaj yeem ua raws li qhov ntev ntawm cov DNA txawv teb chaws nyob nruab nrab ntawm 05-11 kb. Tsis tas li ntawd, nws muaj qhov tshwj xeeb cleavage site rau kev nkag mus rau txawv teb chaws DNA.
Hloov Vectors yog dab tsi?
Hloov vector lossis hloov vector yog hom phage vector tsim los ntawm kev tshem tawm nruab nrab 'filler fragment' cheeb tsam ntawm phage DNA. Qhov xav tau txawv teb chaws DNA ntxig hloov cov phage DNA.
Daim duab 02: Hloov Vector
Hloov cov vectors yog qhov tseem ceeb hauv kev tsim cov tsev qiv ntawv genomic xws li EMBL4 thiab Charon40. Cov vectors no tuaj yeem ua raws li qhov ntev ntawm DNA txawv teb chaws ntawm 08-24kb ntev. Cov cheeb tsam muab tub lim kuj muaj cov noob uas ua rau phage vector tsis tuaj yeem siv tau hauv cov kab mob.
Dab tsi yog qhov zoo sib xws ntawm Kev Ntxig thiab Hloov Vectors?
- Insertion thiab hloov vectors yog phage vectors.
- Ob lub vectors haum rau txawv teb chaws DNA inserts.
- Lawv ob leeg pab tau tsim cov tsev qiv ntawv DNA.
Dab tsi yog qhov txawv ntawm Kev Ntxig thiab Hloov Vectors?
Insertion vs Hloov Vectors |
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| Insertion vector is a type of phage vector that has a restriction site in the phage genome at the site of yeem DNA. | Hloov vector yog hom phage vector tsim los ntawm kev tshem tawm nruab nrab 'filler fragment' cheeb tsam ntawm phage DNA |
| Size of Insert Fragments | |
| 05-11 kb ntev | 08-24 kb ntev |
| No filler fragment | Filler fragment tau hloov los ntawm ib qho txawv teb chaws ntxig |
| Muaj nuj nqi | |
| Ib qho tseem ceeb los tsim cDNA qiv | Ib qho tseem ceeb hauv kev tsim cov tsev qiv ntawv genome |
| Examples | |
| GT10, GT11, thiab Zap yog piv txwv | EMBL4 thiab Charon40 yog piv txwv |
| Cleavage Site | |
| Ib qhov chaw tshwj xeeb cleavage yog tam sim no | Cleavage site muaj cov noob uas tsis tseem ceeb rau lub voj voog lytic |
Summary – Insertion vs Replacement Vectors
Ob qhov ntxig thiab hloov vectors yog ob hom phage vectors. Ob leeg vectors muaj qhov chaw txwv uas pab txhawb kev pabcuam ntawm DNA txawv teb chaws tshiab. Insertion vectors yog qhov tseem ceeb hauv kev tsim cDNA cov tsev qiv ntawv thaum hloov vectors tseem ceeb hauv kev tsim cov tsev qiv ntawv genomic. Tsis tas li ntawd, insertion vectors haum DNA inserts nrog ib tug nruab nrab ntev. Tab sis hloov vectors tuaj yeem ua kom haum ntev dua ntawm cov khoom siv DNA txawv teb chaws. Nyob rau hauv tag nrho, qhov no yog qhov sib txawv tseem ceeb ntawm kev ntxig thiab hloov vectors.
