Qhov Tseem Ceeb - Tris Base vs Tris HCl
Tris puag thiab tris HCl yog cov organic tebchaw uas tuaj yeem siv rau hauv kev npaj cov tshuaj tsis haum. Muaj ntau qhov sib xws nrog rau qhov sib txawv ntawm tris puag thiab tris HCl. Qhov sib txawv ntawm cov qauv tshuaj ntawm ob lub tebchaw yog tias tris HCl muaj HCl molecule txuam nrog tris (hydroxymethyl) aminomethane molecule uas yog feem ntau hu ua tris puag. Qhov sib txawv tseem ceeb ntawm tris puag thiab tris HCl yog tias tris puag muaj cov tshuaj formula C4H11NO3whereas tris HCl muaj tib cov tshuaj formula nrog ib qho ntxiv HCl molecule.
Tris Base yog dab tsi?
Lub sij hawm tris puag yog siv los ua lub npe tris (hydroxymethyl) aminomethane, muaj cov tshuaj formula C4H11NO 3. Nws tseem hu ua THAM. Nws yog ib qho organic compound siv los ua ib feem hauv cov tshuaj tsis haum TAE thiab TBE buffers.
Daim duab 1: Cov Qauv Tshuaj ntawm Tris Base
Cov tshuaj no muaj cov pab pawg amine thawj. Yog li ntawd nws tuaj yeem ua rau cov tshuaj tiv thaiv uas ib txwm muaj thawj amine undergoes; Piv txwv li: condensation tshwm sim nrog aldehydes. Cov yam ntxwv ntawm cov khoom sib xyaw no tshwm sim vim yog pawg amine no. Cov molar mass ntawm no compound yog 121.14 g / mol. Nws yog dawb crystalline hmoov ntawm chav tsev kub thiab siab. Lub melting point ntawm no compound yog nyob ib ncig ntawm 176 ° C, thiab lub boiling point yog 219 ° C.
Cov pH zoo ntawm qhov sib xyaw no yog nyob nruab nrab ntawm 7.5 txog 9.0. Qhov no yog vim lub pKa ntawm conjugated acid ntawm no compound yog 25oC yog 8.07. Qhov tsis tuaj yeem cuam tshuam qee cov enzymes los ntawm kev hloov pH. Lub compound yog npaj industrially los ntawm condensation ntawm nitromethane nrog formaldehyde nyob rau hauv tej yam kev mob.
Tris HCl yog dab tsi?
Tris HCl yog tris hydrochloride uas muaj cov tshuaj formula C4H11NO3 · HCl. Cov molar loj ntawm cov tshuaj no yog 157.59 g / mol. Lub npe IUPAC ntawm tris HCl yog 2-amino-2-(hydroxymethyl)propane-1, 3-diol hydrochloride. Nws tseem hu ua THAM hydrochloride.
Daim duab 2: Cov qauv tshuaj ntawm Tris HCl
Nws yog ib qho organic compound uas feem ntau siv hauv cov kev daws teeb meem xws li TAE thiab TBE. Cov compound no yog dej soluble heev. Qhov sib xyaw no ua haujlwm zoo dua hauv pH ntau ntawm 7.0 txog 9.0. Nws tuaj yeem siv rau hauv kev npaj ntawm Laemmli tsis, uas yog siv hauv SDS-PAGE buffers.
Kev npaj ntawm tris HCl yog ua tiav los ntawm kev sib xyaw tris nrog HCl. Thaum tris sib xyaw nrog tris HCL, cov tshuaj tsis tuaj yeem muab tau, uas yog qhov tseem ceeb heev kom tsis txhob xav tau kev ua haujlwm nrog cov kua qaub muaj zog lossis cov hauv paus muaj zog.
Qhov Zoo sib xws ntawm Tris Base thiab Tris HCl yog dab tsi?
- Ob leeg yog cov khoom hauv TAE thiab TBE cov kev daws teeb meem.
- Ob leeg yog cov organic tebchaw.
Qhov txawv ntawm Tris Base thiab Tris HCl yog dab tsi?
Tris Base vs Tris HCl |
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| Tris puag yog hais txog tris (hydroxymethyl) aminomethane uas muaj cov tshuaj formula (C 4 H 11 NO 3). | Tris HCl yog tris hydrochloride uas muaj cov tshuaj formula C4H11NO3 · HCl. |
| Molar Mass | |
| Cov molar loj ntawm tris yog 121.14 g / mol. | Cov molar loj ntawm tris HCl yog 157.59 g / mol. |
| IUPAC Lub npe | |
| IUPAC lub npe ntawm tris yog tris (hydroxymethyl) aminomethane. | IUPAC lub npe ntawm tris HCl yog 2-amino-2-(hydroxymethyl)propane-1, 3-diol hydrochloride. |
| pH Range | |
| Tris puag ua haujlwm zoo tshaj plaws hauv thaj tsam ntawm 7.5 txog 9.0. | Tris HCl ua haujlwm zoo tshaj plaws hauv thaj tsam ntawm 7.0 txog 9.0. |
Summary – Tris Base vs Tris HCl
Tris puag thiab tris HCl yog cov khoom sib txawv hauv cov kev daws teeb meem sib txawv. Qhov sib txawv tseem ceeb ntawm tris puag thiab tris HCl yog tias tris puag muaj cov tshuaj formula C4H11NO3whereas tris HCl muaj tib cov tshuaj formula nrog ib qho ntxiv HCl molecule.
