Qhov sib txawv tseem ceeb ntawm tris thiab tris puag yog lub ntsiab lus tris hais txog txhua qhov sib xyaw uas muaj cov tshuaj formula C4H11NO 3 koom nrog lwm yam molecule whereas tris puag yog cov tshuaj muaj tshuaj uas muaj cov tshuaj formula C4H11 NO33
Ob nqe lus tris thiab tris puag yog xa mus rau cov tshuaj zoo sib xws uas muaj cov tshuaj formula C4H11NO 3 thiab tshuaj npe tris(hydroxymethyl)aminomethane. Ob lo lus no txawv ntawm ib leeg raws li cov molecules uas cuam tshuam nrog lawv.
Tris yog dab tsi?
Lub sij hawm tris yog hais txog txhua qhov sib xyaw uas muaj cov tris (hydroxymethyl) aminomethane pawg. Feem ntau, tris (hydroxymethyl) aminomethane nws tus kheej yog lub npe hu ua "tris base", tab sis tuaj yeem muaj qee pawg neeg cuam tshuam nrog pab pawg no. Piv txwv li, tris HCl muaj tris (hydroxymethyl) aminomethane hauv kev koom nrog HCl molecule. Tris HCl yog ib qho organic compound uas feem ntau siv hauv kev daws teeb meem xws li TAE thiab TBE. Cov tshuaj no yog dej-soluble heev. Qhov sib xyaw no ua haujlwm zoo dua hauv pH ntau ntawm 7.0 txog 9.0. Nws tuaj yeem siv rau hauv kev npaj ntawm Laemmli tsis, uas yog siv hauv SDS-PAGE buffers.
Daim duab 01: Cov qauv tshuaj ntawm Tris Molecule
Tris Base yog dab tsi?
Tris base los yog tris (hydroxymethyl)aminomethane yog tshuaj lom neeg muaj cov tshuaj formula C4H11NO 3Peb tuaj yeem sau cov ntsiab lus no ua THAM. Tris puag yog cov organic compound uas yog ib qho tseem ceeb raws li ib feem ntawm cov kev daws teeb meem tsis TAE thiab TBE buffers.
Tris puag muaj pawg thawj amine. Yog li no cov tshuaj no tuaj yeem raug cov tshuaj tiv thaiv uas raug rau thawj cov tshuaj amine; Piv txwv li, tris puag tuaj yeem ua rau cov tshuaj tiv thaiv condensation nrog aldehydes. Cov yam ntxwv ntawm tris puag yog los ntawm nws pawg amine. Cov molar loj ntawm tris puag yog 121.14 g / mol, thiab nws tshwm sim dawb crystalline hmoov nyob rau hauv chav tsev kub thiab siab. Tsis tas li ntawd, lub melting point ntawm no compound yog nyob ib ncig ntawm 176 ° C, thiab lub boiling point yog 219 ° C.
Cov pH zoo ntawm tris puag yog nyob nruab nrab ntawm 7.5 txog 9.0. Qhov no yog vim lub fact tias pKa ntawm conjugated acid ntawm no compound yog 25oC yog 8.07. Tsis tas li ntawd, qhov tsis tuaj yeem cuam tshuam qee cov enzymes los ntawm kev hloov pH. Tris puag tuaj yeem npaj kev lag luam los ntawm kev sib xyaw ntawm nitromethane nrog formaldehyde nyob rau hauv cov xwm txheej yooj yim.
Qhov txawv ntawm Tris thiab Tris Base yog dab tsi?
Ob nqe lus tris thiab tris puag yog xa mus rau cov tshuaj zoo sib xws uas muaj cov tshuaj formula C4H11NO 3 thiab tshuaj npe tris(hydroxymethyl)aminomethane. Qhov sib txawv tseem ceeb ntawm tris thiab tris puag yog tias lub sij hawm tris yog hais txog txhua qhov sib xyaw uas muaj cov tshuaj formula C4H11NO 3 nyob rau hauv koom nrog ib co lwm yam molecule whereas tris puag yog cov tshuaj compound uas muaj cov tshuaj formula C4H11NO 3
Hauv qab no yog cov ntsiab lus ntawm qhov sib txawv ntawm tris thiab tris puag
Summary – Tris vs Tris Base
Ob nqe lus tris thiab tris puag yog xa mus rau cov tshuaj zoo sib xws uas muaj cov tshuaj formula C4H11NO 3 thiab tshuaj npe tris(hydroxymethyl)aminomethane. Qhov sib txawv tseem ceeb ntawm tris thiab tris puag yog tias lub sij hawm tris yog hais txog txhua qhov sib xyaw uas muaj cov tshuaj formula C4H11NO 3 nyob rau hauv koom nrog ib co lwm yam molecule whereas tris puag yog cov tshuaj compound uas muaj cov tshuaj formula C4H11NO 3.
