Qhov Tseem Ceeb Qhov txawv - Exome vs Transcriptome
Ib noob muaj coding thiab tsis-coding cheeb tsam hauv nws. Coding sequences hu ua exons, thiab tsis-coding sequences hu ua introns. Cov nucleotide ib ntus ntawm exons ntawm cov noob sawv cev rau cov noob caj noob ces ntawm cov noob los ua ke cov protein tshwj xeeb. Yog li, exons tseem nyob hauv mRNA molecule. Tag nrho cheeb tsam exon ntawm genome yog hu ua exome, thiab nws yog ib feem tseem ceeb ntawm genome. Cov txheej txheem ntawm cov noob caj noob ces hloov mus rau hauv cov kab mob caj ces ntawm mRNA molecule, uas xav tau rau kev tsim cov protein. Tag nrho cov mRNA molecules transcribed nyob rau hauv ib lub xovtooj ntawm tes los yog ib tug cell cell nyob rau hauv ib lub sij hawm yog hu ua ib tug transcriptome. Qhov sib txawv tseem ceeb ntawm exome thiab transcriptome yog tias exome sawv cev rau cov kab ke ntawm cov cheeb tsam exon ntawm cov genome thaum cov ntawv sau qhia txog tag nrho mRNA ntawm lub xov tooj ntawm tes lossis cov ntaub so ntswg ntawm lub sijhawm.
Exome yog dab tsi?
Genes yog tsim los ntawm exons, introns thiab kev tswj hwm ib ntus. Exons yog cov cheeb tsam gene uas tau sau rau hauv mRNA ib ntus thaum lub sijhawm sau ntawv. Introns thiab lwm thaj chaw tsis-coding raug tshem tawm thaum lub sijhawm sau ntawv. Cov nucleotide ib ntus ntawm exons txiav txim siab cov txheej txheem caj ces ntawm cov noob uas ua ke cov protein tshwj xeeb nws cov lej. Tsuas yog exons nyob hauv mRNA ntawm cov protein. Kev sau ntawm exons nyob rau hauv lub genome yog hu ua exome ntawm ib tug kab mob. Nws sawv cev rau ib feem ntawm cov genome uas tau nthuav tawm hauv cov noob. Hauv tib neeg, exome suav 1% los ntawm genome. Nws yog cov protein-coding feem ntawm tib neeg genome.
Daim duab 01: Exome
Daim ntawv teev lus yog dab tsi?
Cov ntawv sau tseg yog sau tag nrho cov protein-coding thiab tsis-coding transcripts (RNAs) hauv cov ntaub so ntswg. Transcriptome sawv cev rau kev sau tag nrho mRNA molecules qhia los ntawm cov noob hauv ib lub xovtooj ntawm tes lossis cov ntaub so ntswg. Cov ntawv sau ntawm ib lub xovtooj tuaj yeem sib txawv nrog cov ntawv sau ntawm lwm hom xovtooj. Cov ntawv sau tseg kuj tseem muaj zog - nws hloov nrog lub sijhawm teb rau ob qho tib si sab hauv thiab sab nraud stimuli. Txawm nyob rau hauv tib cov ntaub so ntswg los yog nyob rau hauv tib hom cell, transcriptome yuav hloov tom qab ob peb feeb.
Transcriptome txawv ntawm exome ntawm lub cev. Transcriptome suav nrog tsuas yog cov exome ua ntu zus. Txawm hais tias qhov exome ntawm ib lub xovtooj tseem zoo ib yam, cov ntawv teev lus sib txawv ntawm cov hlwb vim hais tias cov noob qhia tsis zoo ib yam rau txhua lub hlwb lossis cov ntaub so ntswg. Tsuas yog cov noob tseem ceeb tau nthuav tawm hauv cov hlwb thiab cov ntaub so ntswg sib txawv. Gene qhia yog cov ntaub so ntswg lossis cov txheej txheem ntawm tes. Nws yog tswj los ntawm ntau yam xws li ib puag ncig yam. Yog li ntawd, cov ntaub ntawv sau tseg tuaj yeem sib txawv nrog cov xwm txheej sab nraud.
Transcriptome yog siv los ua tus thawj coj rau kev tshawb fawb proteomics. Tag nrho cov proteins yog muab los ntawm mRNA sequences. Kev hloov pauv kev txhais lus tuaj yeem ua rau muaj kev hloov pauv hauv cov protein. Txawm li cas los xij, transcriptome muab cov ntaub ntawv tseem ceeb rau kev tshawb fawb proteomic.
Daim duab 02: Cov ntaub ntawv sau tseg ntawm embryonic qia hlwb
Dab tsi yog qhov txawv ntawm Exome thiab Transcriptome?
Exome vs Transcriptome |
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| Exome yog qhov sau ntawm cov protein-coding cheeb tsam ntawm cov noob. | Transcriptome yog sau los ntawm txhua tus sau RNA suav nrog mRNA. |
| Sample | |
| Exome yog kawm siv DNA qauv. | Transcriptome yog kawm siv tus qauv RNA. |
| Txoj Kev Kawm | |
| Txhua exome sequencing yog txoj kev kawm exome. | RNA sequencing yog txoj kev kawm transcriptome. |
Summary – Exome vs Transcriptome
Exons yog cov txheej txheem coding ntawm cov noob thiab txiav txim siab mRNA cov kab ke ntawm cov proteins. Kev sau ntawm cov coding sequences (exons) yog hu ua exome ntawm lub cev. Cov noob tau hloov mus rau hauv mRNA molecules ua ntej ua cov protein. Tag nrho mRNA molecules ntawm ib lub xovtooj ntawm tes los yog cov ntaub so ntswg ntawm lub sijhawm twg los tau raug hu ua transcriptome. Transcriptome sawv cev rau cov noob uas tau nthuav tawm rau hauv mRNA txhua lub sijhawm. Transcriptome yog cell thiab cov ntaub so ntswg tshwj xeeb thiab cuam tshuam nrog ib puag ncig. Qhov no yog qhov txawv ntawm exome thiab transcriptome.
