Qhov Tseem Ceeb Qhov Sib Txawv - Kev Hloov Pauv vs Kev Hloov Siab
Kev hloov pauv yog txheej txheem uas koom nrog hauv cov noob hloov pauv ntawm cov hlwb eukaryotic siv cov tshuaj los yog lub cev. Transfection tuaj yeem muab faib ua ob hom tseem ceeb hu ua transient transfection thiab ruaj khov transfection. Thaum lub sij hawm transient transfection, cov noob ntawm kev txaus siab tsis koom ua ke nrog tus tswv tsev genome thiab tau qhia ib ntus hauv tus tswv tsev rau ib lub sij hawm luv, whereas, nyob rau hauv ruaj khov transfection, cov noob ntawm kev txaus siab integrates nrog tus tswv tsev genome thiab yog sustained rau ib tug ntev lub sij hawm nyob rau hauv ob peb tiam.. Qhov no yog qhov sib txawv tseem ceeb ntawm transient thiab ruaj khov transfection. Hauv ob qho xwm txheej, kev hloov pauv tau ua tiav, thiab cov noob tau nthuav tawm.
Kev hloov pauv hloov pauv yog dab tsi?
Kev hloov pauv yog ib qho cuab yeej tseem ceeb rau kev tso cov noob rau hauv eukaryotic hlwb. Ntawm ob hom kev hloov pauv, kev hloov pauv hloov pauv yog ib txoj hauv kev hloov cov noob. Los ntawm ib tug vector, txawv teb chaws noob yog hloov nyob rau hauv lub host cell. Thaum DNA txawv teb chaws nkag mus rau hauv lub xov tooj ntawm tes, nws muaj ob txoj kev xaiv. Nws tuaj yeem ua ke nrog tus tswv tsev genome thiab rov ua dua, lossis nyob hauv yam tsis muaj kev koom ua ke rau hauv genome. Transient transfection nthuav tawm cov lus qhia ib ntus ntawm cov noob caj noob ces yam tsis muaj kev koom ua ke rau hauv tus tswv tsev genome. Genes nthuav qhia thiab tsim cov coded protein kom txog thaum lub cell sib faib. Txawm li cas los xij, vim tsis muaj peev xwm ntawm kev sib koom ua ke, nws tsis tuaj yeem rov ua dua thiab nkag mus rau cov tiam tom ntej. Hom kev hloov pauv no ua tiav rau lub sijhawm luv luv. Thaum lub sij hawm faib cell los yog vim muaj lwm yam, DNA txawv teb chaws raug rau degradation. Transient transfection yog qhia thaum txawv teb chaws DNA nyob rau hauv daim ntawv ntawm coiled DNA heev.
Daim duab 01: Kev Hloov Hloov Hloov
Kev hloov pauv ruaj khov yog dab tsi?
Kev hloov pauv ruaj khov qhia tau tias muaj kev vam meej txawv teb chaws kev koom ua ke rau hauv tus tswv tsev genome. Thaum cov DNA txawv teb chaws nkag mus rau hauv lub xov tooj ntawm tes, ib feem ntawm cov DNA txawv teb chaws koom ua ke nrog tus tswv tsev genome thiab ua ib feem ntawm nws. Li no, txawv teb chaws DNA kuj replicates thiab dhau mus rau yav tom ntej tiam thaum tus tswv tsev genome replicates. Hom kev hloov pauv no yog qhov nyuaj thiab tsis tshua muaj. Txawm li cas los xij, vim qhov ruaj khov hloov mus rau hauv genome, qhov zoo li no tau ruaj khov rau lub sijhawm ntev ntawm ntau tiam neeg.
Kev hloov pauv ruaj khov yog txheej txheem nyuaj thiab yuav tsum muaj kev xa khoom zoo ntawm DNA thiab cell tau txais cov DNA txawv teb chaws rau hauv nws cov genome. Li no, linear DNA nyiam qhov ruaj khov hloov dua li DNA ncig. Txawm li cas los xij, tus nqi hloov pauv ruaj khov yog kwv yees li ib ntawm 10 4hloov pauv hlwb. Kev hloov pauv ruaj khov tuaj yeem pom tau los ntawm cotransfomation ntawm tus cim xaiv tau thiab ua qhov kev xaiv khoom cuav ntawm qhov nruab nrab.
Daim duab 02: Kev hloov pauv ruaj khov
Dab tsi yog qhov sib txawv ntawm Kev Hloov Pauv thiab Kev Ruaj Ntseg?
Kev hloov pauv hloov pauv ruaj khov |
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| DNA txawv teb chaws tsis koom ua ke rau hauv tus tswv tsev genome. | DNA txawv teb chaws koom ua ke nrog tus tswv tsev genome thiab dhau los ua ib feem ntawm nws. |
| Replication in the Host | |
| Cov noob hloov pauv hloov pauv tsis hloov pauv hauv tus tswv tsev. Yog li cov noob tsis raug xa mus rau lwm tiam neeg. | Genes replicated nyob rau hauv tus tswv tsev thiab dhau mus rau tiam tom ntej. |
| Lub sijhawm ntawm Gene Qhia | |
| Genes tau nthuav tawm rau lub sijhawm kawg thiab tom qab ntawd, lawv raug rhuav tshem. | Genes dhau los ua ib feem ntawm cov genome thiab qhia nyob rau hauv tiam neeg rau lub sijhawm ntev. |
| Qhov no muaj txiaj ntsig rau kev kawm txog cov teebmeem ntawm kev qhia luv luv ntawm cov noob lossis cov khoom siv noob. | Qhov no muaj txiaj ntsig zoo rau kev kawm txog cov txiaj ntsig ntawm cov noob caj ces ntev. |
| Nrhiav tau ntawm qhov kev hloov chaw | |
| Genes tau nthuav tawm thiab tuaj yeem tshawb pom tau yooj yim los ntawm kev ntxig tus neeg sau xov xwm. | Kev hloov pauv ruaj khov tuaj yeem tshawb pom tau yooj yim los ntawm kev ntxig tus cim xaiv tau thiab xaiv los ntawm kev xaiv khoom siv hauv xov xwm. |
| tshwm sim thiab txheej txheem | |
| Kev hloov pauv hloov pauv yog qhov tshwm sim thiab tsis nyuaj ua. | Kev hloov pauv ruaj khov tsis tshua muaj tshwm sim thiab nyuaj ua. |
| Nature of DNA | |
| Highly supercoiled DNA yog tsim rau kev hloov pauv mus ib ntus. | Linear DNA yog tsim rau kev hloov pauv ruaj khov. |
| Applications | |
| Qhov no yog siv rau kev tshawb fawb ntawm noob caj noob ces los yog ntsiag to nrog inhibitory RNAs, protein ntau ntawm me me | Qhov no yog siv rau kev tsim cov protein ntau ntawm cov tshuaj loj, kev tshawb fawb tshuaj mus ntev, kev kho noob, kev tshawb fawb txog cov txheej txheem ntawm kev tswj cov caj ces mus ntev |
Summary – Transient vs Stable Transfection
Kev hloov pauv hloov pauv yooj yim thiab ruaj khov yog ob hom kev hloov pauv tau pom thaum lub sijhawm hloov pauv mus rau hauv cov hlwb eukaryotic los ntawm cov kab mob uas tsis yog kab mob. Hloov pauv DNA txawv teb chaws tsis koom nrog tus tswv tsev genome hauv kev hloov pauv hloov pauv thaum nws koom nrog tus tswv tsev genome hauv kev hloov pauv ruaj khov. Circular coiled DNA tau pom qhov hloov pauv hloov pauv thaum cov kab DNA nyiam qhov hloov pauv ruaj khov. Qhov no yog qhov sib txawv ntawm transient thiab ruaj khov transfection. Txawm li cas los xij transient transfection yog ntau dua thiab yooj yim piv rau kev hloov pauv ruaj khov. Tab sis kev xaiv ntawm ib qho ntawm ob yog nyob ntawm lub hom phiaj ntawm kev hloov cov noob thiab lub sijhawm ntawm txoj haujlwm tshawb fawb.
