Qhov sib txawv tseem ceeb ntawm C18 thiab phenyl kab yog qhov sib cais muab los ntawm C18 HPLC kab qis dua piv rau cov phenyl HPLC kab.
C18 kem hauv HPLC yog ib kem uas siv cov tshuaj C18 raws li cov theem nyob ruaj khov, thaum lub phenyl kem yog ib hom kab uas tuaj yeem pom hauv qee cov cuab yeej HPLC. Nws muaj luv luv alkyl phenyl ligands uas yog covalently khi rau silica nto.
Kab C18 yog dab tsi?
A C18 kem hauv HPLC yog ib kem uas siv C18 cov khoom ua haujlwm nyob ruaj khov. Yog li, cov no yog hu ua C18 HPLC kab. Cov no muaj txiaj ntsig zoo hauv kev tshawb fawb ib puag ncig thiab tshuaj tshuaj lom neeg. Tsis tas li ntawd, C18 HPLC kab yog qhov tseem ceeb hauv kev lag luam tshuaj thiab kev tshawb fawb ib puag ncig rau kev tshuaj xyuas ntawm ib tus neeg ntawm cov tshuaj sib tov.
Daim duab 01: Ib Hom HPLC Kem
Feem ntau, cov kab C18 no siv octaldecylsilane, thiab cov khoom no muaj 18 carbon atoms uas khi rau silica. Qhov no txhais tau hais tias qhov sib xyaw no muaj cov pa roj carbon ntau dua thiab cov saw hlau ntev dua li C-8. Cov pa roj carbon atoms ntxiv hauv cov saw hlau no ua rau thaj chaw loj heev rau lub sijhawm txawb mus hla.
Feem ntau, peb tuaj yeem siv C18 kem hauv HPLC ua kab rov qab-theem. Qhov no yog vim hais tias hom kab no siv ntau cov kuab tshuaj polar (xws li dej, methanol, thiab acetonitrile). Tsis tas li ntawd, cov theem nyob ruaj khov yog cov tsis muaj polar hydrocarbon (C18).
Phenyl Kem yog dab tsi?
A phenyl kem yog ib hom kab uas tuaj yeem pom hauv qee cov cuab yeej HPLC uas muaj luv luv alkyl phenyl ligands covalently khi rau silica nto. Txawm li cas los xij, hauv cov kab HPLC niaj hnub no, peb tuaj yeem nrhiav cov theem diphenyl uas tau tsim los txhim kho pi-pi kev sib cuam tshuam. Muaj qhov luv luv alkyl txuas, yog li cov kab phenyl feem ntau tsis muaj hydrophobic tuav, thiab nws pom tias tsis tshua muaj hydrolytic stability.
Daim duab 02: Ib qho piv txwv HPLC Pump
Phenyl kab muaj kev vam meej hauv kev sib cais qhov chaw isomers, tocopherols, flavonoids, polynuclear aromatics, nitroaromatic compounds, cov khoom xyaw tshuaj nquag, thiab lwm yam sib txuas.
Qhov txawv ntawm C18 thiab Phenyl Kab yog dab tsi?
HPLC ntsuas muaj ib kem uas muaj txiaj ntsig zoo hauv kev sib cais cov khoom sib xyaw. Muaj ntau hom kab hauv HPLC cov cuab yeej. C18 kem thiab phenyl kem yog ob kem. Qhov sib txawv tseem ceeb ntawm C18 thiab phenyl kem yog tias kev sib cais muab los ntawm C18 HPLC kab yog qis dua piv rau phenyl HPLC kab. Ntxiv mus, qhov kev daws teeb meem muab los ntawm phenyl kem yog siab dua piv rau C18 kem. Tsis tas li ntawd, cov theem nyob ruaj khov ntawm C18 kem yog C18 compound xws li octaldecylsilane, thaum lub sij hawm nyob ruaj khov ntawm phenyl kem yog ua los ntawm luv luv alkyl phenyl ligands uas yog covalently khi rau ntawm silica nto.
Cov hauv qab no infographic nthuav tawm qhov sib txawv ntawm C18 thiab phenyl kem hauv daim ntawv tabular rau ib sab ntawm kev sib piv.
Summary – C18 vs Phenyl Kab
Kab C18 hauv HPLC yog ib kem uas siv cov tshuaj C18 raws li theem nyob ruaj khov. Lub caij no, phenyl kem yog ib hom kab uas tuaj yeem pom nyob rau hauv qee cov cuab yeej HPLC uas muaj luv luv alkyl phenyl ligands uas yog covalently khi rau silica nto. Qhov sib txawv tseem ceeb ntawm C18 thiab phenyl kab yog qhov sib cais muab los ntawm C18 HPLC kab qis dua li ntawm phenyl HPLC kab.
