Qhov sib txawv tseem ceeb ntawm protoplasts thiab spheroplast yog tias protoplasts yog cov nroj tsuag lossis cov kab mob microbial tsim los ntawm stripping phab ntsa cell tag, thaum spheroplast yog cov nroj tsuag lossis cov kab mob microbial tsim los ntawm stripping phab ntsa cell ib nrab.
Ib phab ntsa ntawm tes yog txheej txheej txheej txheem thiab tiv thaiv uas nyob ib puag ncig qee hom cell. Nws yog nyob rau sab nraud ntawm lub cell membrane. Nws tuaj yeem yog tawv, hloov tau, lossis nruj. Lub cell phab ntsa ib txwm muab kev txhawb nqa thiab kev tiv thaiv rau lub hlwb. Ntxiv mus, nws tuaj yeem ua raws li kev lim dej. Cov phab ntsa ntawm tes tsis muaj nyob hauv cov tsiaj hlwb. Tab sis cov phab ntsa ntawm tes muaj nyob rau hauv lwm yam kab mob xws li algae, fungi, nroj tsuag, thiab kab mob. Protoplasts thiab spheroplast yog ob hom kev hloov pauv ntawm cov nroj tsuag lossis cov kab mob microbial uas cov phab ntsa ntawm tes tau raug tshem tawm tag nrho lossis ib nrab tshem tawm.
Protoplasts yog dab tsi?
Protoplasts yog tsim los ntawm kev tshem tawm cov phab ntsa ntawm tes los ntawm cov nroj tsuag, cov kab mob los yog cov kab mob fungal los ntawm kev siv tshuab, tshuaj, los yog enzymatic txhais tau tias. Protoplast yog ib lo lus lom neeg uas tau tsim los ntawm Hanstein thawj zaug hauv xyoo 1880. Muaj cov enzymes sib txawv uas muaj thaum cais cov protoplast los ntawm enzymatic txhais tau tias. Cov phab ntsa ntawm tes feem ntau yog ua los ntawm ntau hom polysaccharides. Cov protoplasts tuaj yeem tsim los ntawm degrading cov phab ntsa ntawm tes nrog kev sib xyaw ntawm cov enzymes tsim nyog polysaccharide degrading. Piv txwv ntawm qee cov enzymes no yog cellulase, pectinase, xylanase (cov hlwb cog), lysozyme, N, O-diacetylmuramidase, lysostaphin (gram-positive bacteria), thiab chitinase (cov kab mob fungal). Tom qab kev zom ntawm cov phab ntsa ntawm tes, cov protoplast yuav nkag siab zoo rau osmotic kev nyuaj siab. Yog li ntawd, kev zom cov phab ntsa ntawm tes thiab cov khoom siv protoplast yuav tsum tau ua hauv cov tshuaj isotonic los tiv thaiv kev tawg ntawm cov plasma membrane.
Daim duab 01: Protoplasts
Tsis tas li ntawd, protoplasts yog cov cuab yeej tshawb fawb muaj txiaj ntsig uas tuaj yeem siv rau ntau yam kev siv. Cov ntawv thov ntawm cov protoplasts yog kev kawm txog cov kab mob ntawm daim nyias nyias, kev hloov pauv ntawm somaclonal hauv cov nqaij mos, hloov DNA, cog qoob loo (hybrid cov ntaub so ntswg kab lis kev cai), thiab fluorescence-activated cell sorting (FACS).
Spheroplast yog dab tsi?
Spheroplasts yog cov nroj tsuag lossis cov kab mob microbial tsim los ntawm stripping cell phab ntsa ib nrab. Spheroplast feem ntau yog tsim los ntawm cov kab mob xws li kab mob gram-tsis zoo thiab cov kab mob fungal xws li cov poov xab. Spheroplast tsuas khaws ib feem ntawm lawv cov phab ntsa ntawm tes. Nyob rau hauv cov ntaub ntawv ntawm gram-tsis zoo kab mob spheroplats, lub peptidoglycan tivthaiv ntawm lub cell phab ntsa raug tshem tawm, tab sis cov txheej membrane tivthaiv tsis tau muab tshem tawm. Cov txheej txheem uas yog siv los tsim spheroplast nyob ntawm seb hom cell. Cov kab mob fungal tuaj yeem tsim tom qab kev kho mob chitinase, lyticase lossis β glucuronidase, thaum cog cov hlwb tsim spheroplast tom qab kho nrog pectinase, cellulase, thiab xylanase. Ntxiv mus, cov kab mob spheroplast yog tsim tom qab kev kho mob nrog tshuaj tua kab mob xws li fosfomycin, vancomycin, moenomycin, lactivicin, thiab beta-lactam tshuaj tua kab mob. Cov kab mob Gram-tsis zoo kuj tuaj yeem kho nrog lysozyme nyob rau hauv lub xub ntiag ntawm EDTA los tsim spheroplast.
Daim duab 02: Spheroplasts
Kev siv ntau yam ntawm spheroplast suav nrog kev tshawb pom cov tshuaj tua kab mob uas inhibit cell phab ntsa biosynthesis, kawm txog kev ua haujlwm ntawm cov kab mob ion channel los ntawm cov txheej txheem hu ua patch clamping, transfection ntawm cov tsiaj hlwb, thiab kev yooj yim ntawm cell lysis.
Dab tsi yog qhov zoo sib xws ntawm Protoplasts thiab Spheroplast?
- Protoplasts thiab spheroplast yog ob hom kev hloov pauv ntawm cov nroj tsuag lossis cov kab mob microbial uas cov phab ntsa ntawm tes tau raug tshem tawm tag nrho lossis ib nrab tshem tawm.
- Ob leeg yog kheej kheej.
- Ob leeg yog cov rhiab heev rau osmotic thiab mechanical shock.
- Lub cell phab ntsa zom thiab khaws cia ntawm ob qho tib si yuav tsum tau ua hauv cov tshuaj isotonic kom tiv thaiv kev tawg ntawm cov plasma membrane.
- Lawv tau npaj ib txwm nyob hauv chav kuaj.
- Lawv muaj ntau yam kev siv.
Qhov txawv ntawm Protoplasts thiab Spheroplast yog dab tsi?
Protoplasts yog cov nroj tsuag lossis cov kab mob microbial tsim los ntawm kev tshem tawm cov phab ntsa ntawm tes kom tiav, thaum spheroplast yog cov nroj tsuag lossis cov kab mob microbial tsim los ntawm stripping phab ntsa cell ib nrab. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm protoplasts thiab spheroplast. Tsis tas li ntawd, protoplasts yog bounded los ntawm ib daim nyias nyias, thaum spheroplast yog bounded los ntawm ob daim nyias nyias.
Cov hauv qab no infographic nthuav tawm qhov sib txawv ntawm cov protoplasts thiab spheroplast hauv daim ntawv tabular rau ib sab ntawm kev sib piv.
Summary – Protoplasts vs Spheroplats
Protoplasts thiab spheroplast xa mus rau cov ntaub ntawv hloov pauv ntawm cov nroj tsuag, kab mob lossis cov kab mob fungal. Protoplasts yog cov nroj tsuag lossis cov kab mob microbial tsim los ntawm stripping phab ntsa ntawm tes kom tiav, thaum spheroplast yog cov nroj tsuag lossis cov kab mob microbial tsim los ntawm stripping cell phab ntsa ib nrab. Yog li, qhov no qhia txog qhov sib txawv ntawm protoplasts thiab spheroplast.
