Qhov sib txawv tseem ceeb ntawm lambda phage thiab M13 phage yog tias lambda phage yog lub taub hau mus rau tus Tsov tus tw bacteriophage uas muaj ib txoj kab ob-stranded genome thaum M13 phage yog filamentous bacteriophage uas muaj ib ncig ib leeg-stranded genome.
Viruses yog tus kab mob sib kis. Lawv kis tau cov nroj tsuag, tsiaj txhu, fungi, thiab kab mob. Bacteriophages yog cov kab mob kab mob. Lawv tshwj xeeb kis cov kab mob. Hauv molecular biology, bacteriophages yog siv los ua cov noob xa khoom rau eukaryotes. Lambda phage thiab M13 phage yog cov feem ntau kawm thiab siv phages ntawm txawv bacteriophages. Lawv tsis qhia tropism rau eukaryotic hlwb. Tsis tas li ntawd, lawv qhia kev ruaj ntseg hauv cov xwm txheej hnyav. Lawv kuj yooj yim rau manipulate thiab loj ntau lawm.
Lambda Phag yog dab tsi?
Lambda phage yog bacteriophage uas kis tau Eschechia coli. Lwm lub npe ntawm tus kab mob no yog Escherichia phage lambda, coliphage lambda thiab Escherichia virus lambda. Nws yog lub taub hau rau tailed bacteriophage. Nws muaj ib tug linear ob-stranded genome. Txhua qhov kawg ntawm genome yog 12 bp ntev overhang. Lub genome loj ntawm tus kab mob no yog hais txog 48 kb, thiab txoj kab uas hla ntawm icosahedral protein capsid yog nyob ib ncig ntawm 55 nm. Tsis tas li ntawd, qhov ntev ntawm tus Tsov tus tw ntawm tus kab mob no yog nyob ib ncig ntawm 145 nm. Tus kab mob no tau tshawb pom los ntawm Esther Lederberg xyoo 1950. Nws yog tus kab mob temperate. Nws tuaj yeem ua rau ob qho tib si lytic thiab lysogenic cycles. Thaum lub sij hawm lysogenic hom, lambda phage muaj nyob rau hauv prophage yam tsis ua mob rau tus tswv tsev kab mob.
Daim duab 01: Lambda Phag
Lambda phage yog qhov muaj txiaj ntsig zoo li vector hauv kev xa cov noob rau cov hlwb eukaryotic. Nws yog siv los ua cloning vector hauv kev tsim kho tsev qiv ntawv genomic. Tab sis lambda phage vector tsuas tuaj yeem xa qhov loj ntawm 35-50 kb ntawm DNA. Nov yog ib qho kev txwv ntawm tus kab mob no ua tus vector.
M13 Phaj yog dab tsi?
M13 phage yog cov kab mob bacteriophage uas yog tus tswv cuab ntawm tsev neeg filamentous bacteriophage. Nws kis tau E. coli. M13 phage muaj ib leeg-stranded zoo-sensitive ncig genome. Nws genome loj yog hais txog 6.4 kb, thiab nws codes rau kaum genes. Nws yog ib qho yooj yim genome.
Daim duab 02: M13 Phag
Lub capsid ntawm M13 phage tuaj yeem txuas ntxiv los ntawm kev ntxiv cov protein subunits ntxiv. Lub capsid yog helical nyob rau hauv cov duab. Zoo ib yam li lambda phage, M13 kuj tseem muaj txiaj ntsig cloning vector. Nws yog ib qho ntawm thawj vectors tsim rau molecular cloning. Qhov loj ntawm DNA ntxig yog 12 kb rau M13.
Dab tsi zoo sib xws ntawm Lambda Phage thiab M13 Phage?
- Ob leeg Lambda phage thiab M13 phage yog cov kab mob bacteriophages lossis cov kab mob kab mob uas kis kab mob tshwj xeeb.
- Lawv tus kab mob yog Escherichia coli.
- Lawv yog cloning vectors feem ntau siv nyob rau hauv recombinant DNA technology thiab genetic engineering.
- Lawv ua raws li cov noob xa khoom tsheb hauv molecular biology.
- Yog li ntawd, ob qho tib si phages yog thawj cov vectors tsim rau molecular cloning.
Qhov txawv ntawm Lambda Phage thiab M13 Phag yog dab tsi?
Lambda phage yog lub taub hau rau tailed bacteriophage uas muaj ib txoj kab ob-stranded genome, thaum M13 phage yog filamentous bacteriophage uas muaj ib leeg-stranded ncig genome. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm lambda phage thiab M13 phage. Qhov loj ntawm lambda phage genome yog li 48kb, thaum qhov loj ntawm M13 genome yog li 6.4 kb.
Cov hauv qab no infographic teev qhov sib txawv ntawm lambda phage thiab M13 phage nyob rau hauv daim ntawv tabular rau ib sab ntawm kev sib piv.
Summary – Lambda Phage vs M13 Phage
Lambda phage yog lub taub hau rau tailed bacteriophage. Nws muaj ib tug linear ob-stranded genome. Nyob rau hauv sib piv, M13 phage yog ib tug filamentous bacteriophage thiab muaj ib ncig ib leeg-stranded genome. Yog li, qhov no yog qhov txawv ntawm lambda phage thiab M13 phage. Ob leeg bacteriophages kis tau E. coli. Ob lub phages no tau dav siv los ua cloning vectors.
