Qhov sib txawv tseem ceeb ntawm cov cim mesenchymal thiab cov cim qia cell yog tias cov cim mesenchymal yog los ntawm ntau lub zog mesoderm muab tau los ntawm cov hlwb progenitor thaum qia cell cim los ntawm embryonic qia hlwb lossis pluripotent qia cell nto.
Mesenchymal qia hlwb lossis MSCs yog cov neeg laus qia hlwb uas muaj ntau lub zog. Cov MSCs no ua rau muaj ntau hom cell hauv cov nqaij pob txha, uas suav nrog adipocytes, pob txha, tendons, pob txha mos, ligaments, thiab cov leeg. Lawv kuj sib txawv rau hauv lub plawv cov leeg hlwb, cov hlwb endothelial, pancreatic islet hlwb, thiab cov hlab ntsha. Stem cells yog cov tsis muaj kev faib cov hlwb uas ua rau muaj ntau hom cell sib txawv. Stem cells paub tias sau lossis hloov cov ntaub so ntswg xws li ntshav, pob txha, gametes, epithelia, paj hlwb, cov leeg, thiab lwm yam ntaub so ntswg nrog cov hlwb tshiab. Ob leeg MSCs thiab qia hlwb muaj cov cim txhawm rau txhawm rau cais, txheeb xyuas thiab ua tus yam ntxwv ntawm cov hlwb rau kev siv tshuaj kho mob ntawm tes hauv cov tshuaj kho dua tshiab.
Mesenchymal Markers yog dab tsi?
Mesenchymal cov cim yog ib pawg ntawm cov cim ntawm tes. Lawv tam sim no los yog tsis muaj nyob rau hauv mesenchymal qia hlwb, uas yog siv los cais thiab txheeb xyuas multipotent mesoderm-derived progenitor hlwb (mesenchymal qia hlwb). Cov hlwb mesenchymal no muaj peev xwm sib txawv rau hauv cov hlwb uas ua rau adipose, pob txha, pob txha mos, thiab cov leeg nqaij. Lawv muab lub peev xwm dav hauv kev txhim kho ntawm cov kev kho mob ntawm tes. Cov kab mob mesenchymal raug txheeb xyuas los ntawm cov cim ntawm tes uas suav nrog CD10, CD13, CD19, CD29, CD31, CD34, CD44, CD90, CD49a-f, CD51, CD73, CD105, CD106, CD166, thiab Stro-1. Cov no kuj tsis muaj cov nyhuv immunogenic thiab muaj peev xwm los hloov cov ntaub so ntswg puas. Cov yam ntxwv no txhawb kev sib cais thiab kev ua haujlwm ntawm cov cim mesenchymal.
Daim duab 01: Mesenchymal Markers
Mesenchymal cov cim yog ntawm ob pawg: cov cim ib leeg thiab cov cim stemness. Cov cim cim ib leeg yog txaus los txheeb xyuas lossis ntxuav cov qia hlwb mesenchymal zoo li cov hlwb hauv cov kab mob vitro. Stemness markers muaj peev xwm txheeb xyuas cov kab mob mesenchymal qia hlwb nrog cov colony siab tsim los yog txheeb xyuas cov embryonic qia cell zoo li cov hlwb. Cov cim no yog qhov zoo thiab tsis zoo raws li lawv cov lus qhia.
Stem Cell Markers yog dab tsi?
Cov cim kab mob qia cell yog cov noob thiab lawv cov proteins uas siv los cais thiab txheeb xyuas cov qia hlwb. Stem cells kuj raug txheeb xyuas los ntawm kev soj ntsuam ua haujlwm. Cov kev ntsuam xyuas no yog hu ua tus qauv kub rau kev txheeb xyuas thiab kho lub hom phiaj. Txawm hais tias cov kev ntsuam xyuas ua haujlwm ua tau zoo tshaj plaws rau kev txheeb xyuas cov qia hlwb, cov cim molecular lossis cov cim qia cell muab ib txoj hauv kev hauv kev txheeb xyuas cov qia hlwb. Cov cim no yog tsim nyob rau hauv embryonic qia hlwb los yog pluripotent qia hlwb.
qia cell marker profiles hloov pauv nyob ntawm keeb kwm, hom, thiab thiaj li hu ua stemness ntawm qia cell pejxeem. Ob yam tseem ceeb ntawm cov cim no yog cov qauv qhia thiab lub sijhawm. Cov no pab txhawb kev txheeb xyuas kom zoo, cais tawm, thiab ua tus cwj pwm ntawm cov qia hlwb. Ob peb ntawm cov qia hlwb no yog embryonic, mesenchymal / stromal, hematopoietic, thiab neural qia hlwb. Qee qhov piv txwv ntawm cov tshuaj tiv thaiv kab mob qia cell yog CD31 cov tshuaj tiv thaiv, CD4 tshuaj tiv thaiv, Nestin cov tshuaj tiv thaiv, Neurofilament antibodies, SOX2 antibodies, OCT4 antibodies. Cov kab mob qia cell no muaj peev xwm ua rau muaj kev tiv thaiv kab mob.
Dab tsi yog qhov zoo sib xws ntawm Mesenchymal Markers thiab Stem Cell Markers?
- Mesenchymal cov cim thiab cov cim ntawm cov qia hlwb yog cov proteins.
- Ob lub cim siv ib txoj hauv kev rau kev lees paub thiab kev txiav txim siab ntawm cov hlwb los ntawm pawg sib txawv (CD) raws tu qauv.
Qhov txawv ntawm Mesenchymal Markers thiab Stem Cell Markers yog dab tsi?
Mesenchymal cov cim muaj cov cim zoo thiab tsis zoo raws li lawv cov lus qhia, thaum cov cim ntawm qia cell tsuas muaj ib hom tshuaj tiv thaiv kab mob. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm cov cim mesenchymal thiab qia cell markers. Ntxiv mus, qia cell markers muaj cov nyhuv immunogenic, thaum cov cim mesenchymal tsis muaj cov nyhuv immunogenic ntawm provoking tiv thaiv kab mob. Yog li, qhov no yog lwm qhov sib txawv tseem ceeb ntawm cov cim mesenchymal thiab qia cell markers.
Cov hauv qab no infographic qhia txog qhov sib txawv ntawm cov cim mesenchymal thiab cov cim ntawm cov qia hauv daim ntawv.
Summary – Mesenchymal Markers vs Stem Cell Markers
Mesenchymal cov cim yog ib pawg ntawm cov cim ntawm tes uas muaj los yog tsis muaj nyob rau hauv mesenchymal qia hlwb. Lawv yog siv los cais thiab txheeb xyuas ntau lub zog mesoderm-derived progenitor cells (mesenchymal qia hlwb). Stem cell markers yog cov noob thiab lawv cov proteins uas siv los cais thiab txheeb xyuas cov qia hlwb. Stem cells kuj raug txheeb xyuas los ntawm kev soj ntsuam ua haujlwm. Mesenchymal markers yog ob pawg: cov cim ib leeg thiab stemness markers. Stem cell marker profiles hloov pauv nyob ntawm keeb kwm, hom, thiab thiaj li hu ua stemness ntawm qia cell pejxeem. Ob lub cim yog siv nyob rau hauv daim ntawv thov kev kho mob ntawm tes. Yog li, qhov no yog cov ntsiab lus ntawm qhov sib txawv ntawm cov cim mesenchymal thiab qia cell markers.
