Qhov sib txawv tseem ceeb ntawm kev ntws cytometry thiab immunohistochemistry yog qhov ntws cytometry yog cov txheej txheem uas siv lub teeb laser los kuaj thiab ntsuas lub cev thiab tshuaj lom neeg ntawm cov neeg ntawm cov hlwb lossis cov khoom, thaum immunohistochemistry yog cov txheej txheem siv monoclonal thiab polyclonal antibodies los kuaj xyuas cov antigens tshwj xeeb hauv cov ntaub so ntswg.
Flow cytometry thiab immunohistochemistry yog ob txoj hauv kev uas siv los kuaj cov kab mob, tshwj xeeb yog mob qog noj ntshav. Flow cytometry siv lub laser beam los ua tus yam ntxwv ntawm lub cev thiab tshuaj lom neeg ntawm cov hlwb. Immunohistochemistry siv cov tshuaj tiv thaiv kab mob monoclonal thiab polyclonal rau kev tshawb nrhiav cov antigens tshwj xeeb hauv cov ntaub so ntswg.
Flow Cytometry yog dab tsi?
Flow cytometry yog cov txheej txheem nrov hauv cell biology. Cov txheej txheem no tshawb pom thiab ntsuas cov yam ntxwv ntawm lub cev thiab tshuaj lom neeg ntawm cov cell. Flow cytometry yog dav siv nyob rau hauv kev tiv thaiv kab mob, molecular biology, bacteriology, virology, mob qog noj ntshav thiab kuaj kab mob sib kis. Nws siv lub laser ua lub teeb ci los suav, txheeb, thiab cov hlwb hauv cov kua dej sib tov. Yog li ntawd, nws muab kev tshuaj ntsuam xyuas ntau yam ntawm cov hlwb hauv kev daws teeb meem.
Daim duab 01: Flow Cytometry
Cov txheej txheem no pib nrog kev txhaj tshuaj ntawm tes mus rau hauv qhov ntws cytometer. Flow cytometer muaj peb lub hauv paus tseem ceeb: fluidics (ib lub xovtooj ntawm tes), optics (ntau cov ntxaij lim dej, lub teeb pom kev, thiab lub teeb pom kev zoo), thiab electronics (flow cytometer instrumentation). Ua ntej ntawd, cov qauv yuav tsum tau kho nrog cov dyes tshwj xeeb nyob ntawm cov hlwb raug tshuaj xyuas. Yog li ntawd, nws siv ntau hom fluorescent reagents xws li fluorescently conjugated antibodies, DNA binding dyes, viability dyes, ion indicator dyes thiab fluorescent expression proteins, thiab lwm yam. Qhov ntsuas no tso cai rau ntws ntawm ib lub cell ntawm ib lub sij hawm los ntawm lub laser beam. Thaum lub teeb tawg los ntawm lub xovtooj ntawm tes thiab nws cov khoom, nws emits bands sib txawv wavelengths. Ua li no, kaum tawm txhiab lub hlwb tuaj yeem kuaj xyuas sai, thiab cov ntaub ntawv sau tau raug ua tiav los ntawm lub computer.
Immunohistochemistry yog dab tsi?
Immunohistochemistry yog ib qho txheej txheem uas siv cov tshuaj tiv thaiv kab mob monoclonal thiab polyclonal los txiav txim cov ntaub so ntswg ntawm cov tshuaj tiv thaiv kev txaus siab. Nws yog ib qho txheej txheem hauv histopathology. Nws yog cov txheej txheem microscopy, immunostaining. Cov txheej txheem no pab txhawb kev xaiv kev txheeb xyuas thiab thaj chaw ntawm cov antigens hauv cov hlwb ntawm cov ntaub so ntswg raws li kev khi tshwj xeeb nrog cov tshuaj tiv thaiv fluorescently sau npe. Cov txheej txheem no tau siv dav rau kev kuaj mob qog noj ntshav vim tias cov qog qog nqaij hlav tshwj xeeb tau hais tawm de novo los yog tswj hwm hauv qee cov qog nqaij hlav. Cov txheej txheem no muaj txiaj ntsig zoo hauv kev kwv yees kho cov lus teb hauv ob lub qog tseem ceeb, piv txwv li carcinoma ntawm lub mis thiab prostate. Ntxiv nrog rau kev kuaj kab mob, immunohistochemistry yog siv rau hauv kev tsim tshuaj thiab kev tshawb fawb lom. Ntxiv mus, immunohistochemistry muaj txiaj ntsig zoo thaum kuaj pom thiab lees paub cov kab mob sib kis hauv cov ntaub so ntswg.
Daim duab 02: Immunohistochemistry
Cov txheej txheem no yuav tsum tau muab cov tshuaj biopsies, thiab lawv tau ua tiav rau hauv ntu nrog microtome, thiab tom qab ntawd cov ntu tau tsim nrog cov tshuaj tiv thaiv tsim nyog. Cov chaw ntawm kev khi ntawm cov tshuaj tiv thaiv kab mob yog pom nyob rau hauv lub teeb lossis fluorescent microscope.
Dab tsi zoo sib xws ntawm Flow Cytometry thiab Immunohistochemistry?
- Flow cytometry thiab immunohistochemistry yog ob txoj kev siv tshuaj fluorescently sau tshuaj tiv thaiv.
- Ob txoj kev tuaj yeem ntes cov tshuaj tiv thaiv ntawm lub xov tooj ntawm tes lossis hauv cov hlwb.
- Li no, ob txoj kev tso cai rau kev kuaj kab mob, tshwj xeeb yog kis kab mob thiab mob qog noj ntshav.
Qhov txawv ntawm Flow Cytometry thiab Immunohistochemistry yog dab tsi?
Flow cytometry yog cov txheej txheem laser-raws li tshawb pom thiab ntsuas cov yam ntxwv ntawm lub cev thiab tshuaj lom neeg ntawm cov cell. Immunohistochemistry yog ib txoj hauv kev siv microscopy uas tso cai rau kev txheeb xyuas thiab kev txheeb xyuas cov antigens hauv cov hlwb ntawm cov ntaub so ntswg. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm kev ntws cytometry thiab immunohistochemistry. Ntxiv mus, ntws cytometry siv lub laser beam, thaum immunohistochemistry xav tau cov tshuaj tiv thaiv. Flow cytometer yog cov cuab yeej tseem ceeb uas yuav tsum tau ua hauv cov cytometry ntws, thaum immunohistochemistry xav tau lub teeb lossis fluorescent microscope.
Hauv qab no yog cov ntsiab lus ntawm qhov sib txawv ntawm qhov ntws cytometry thiab immunohistochemistry hauv daim ntawv tabular.
Summary – Flow Cytometry vs Immunohistochemistry
Flow cytometry xav tau lub cytometer ntws, thaum immunohistochemistry xav tau ib qho zoo tib yam lossis fluorescent microscope. Ntxiv mus, ntws cytometry siv lub laser beam, thaum immunohistochemistry siv monoclonal thiab polyclonal antibodies. Tsis tas li ntawd, tus nqi ntawm immunohistochemistry yog qhov tsawg dua piv rau cov cytometry ntws. Yog li, qhov no yog cov ntsiab lus ntawm qhov sib txawv ntawm qhov ntws cytometry thiab immunohistochemistry. Ob txoj kev no muaj txiaj ntsig zoo hauv kev kuaj mob xws li mob qog noj ntshav thiab kab mob sib kis.
