Qhov Tseem Ceeb - Immunofluorescence vs Immunohistochemistry
Kev kuaj kab mob, uas siv cov txheej txheem molecular biological, tau dhau los ua thaj chaw tshiab ntawm kev kuaj mob thev naus laus zis. Nws suav nrog tag nrho cov kev sim thiab cov txheej txheem los txheeb xyuas tus kab mob thiab nkag siab qhov ua rau muaj tus kab mob los ntawm kev tshuaj xyuas DNA, RNA lossis cov protein uas tau hais hauv lub cev. Kev nce qib sai sai hauv kev kuaj mob molecular tau ua rau muaj kev tshawb fawb yooj yim ntawm cov kab mob sib kis thiab tsis sib kis. Cov no yog siv los txiav txim qhov kev hloov pauv hauv ntu lossis qhia qib hauv cov noob tseem ceeb lossis cov protein uas cuam tshuam nrog cov kab mob. Immunofluorescence (IF) thiab Immunohistochemistry (IHC) yog ob txoj kev siv dav hauv kev mob qog noj ntshav. IF yog ib hom IHC uas siv txoj kev tshawb nrhiav fluorescence los txheeb xyuas cov tshuaj tiv thaiv kab mob monoclonal thiab polyclonal, thaum IHC siv cov tshuaj raws li txoj hauv kev los txheeb xyuas cov tshuaj tiv thaiv monoclonal thiab polyclonal. Qhov no yog qhov sib txawv tseem ceeb ntawm IF thiab IHC.
Immunofluorescence (IF) yog dab tsi?
Immunofluorescence yog cov txheej txheem tshawb nrhiav qhov twg cov tshuaj tiv thaiv siv hauv qhov kev ntsuam xyuas tau sau npe siv fluorescent dyes lossis fluorescent proteins rau lub hom phiaj tshawb nrhiav. Cov tshuaj tiv thaiv kab mob thib ob tuaj yeem ua rau tsis xav tau keeb kwm yav dhau los; yog li ntawd, IF txheej txheem yog ua raws li kev sau npe cov tshuaj tiv thaiv thawj zaug nws tus kheej tam sim no txhawm rau kom tsis txhob muaj cov cim tsis xav tau thaum kuaj pom. Los ntawm cov txheej txheem no, tsis yog tshwj xeeb khi ntawm thawj thiab thib ob cov tshuaj tiv thaiv kab mob tiv thaiv, thiab nws nrawm dua vim tias tsis muaj qhov cuam tshuam thib ob cuam tshuam. Cov ntaub ntawv zoo kuj tseem txhim kho.
Daim duab 01: Ob chav immunofluorescence staining rau BrdU, NeuN, thiab GFAP
Fluorochromes lossis fluorescent dyes yog cov khoom sib txuas uas tuaj yeem nqus tau hluav taws xob, nyiam dua ultra violet hluav taws xob uas zoo siab. Thaum cov khoom mus txog hauv av hauv lub xeev los ntawm lub xeev zoo siab, lawv emission hluav taws xob uas yog ntes thiab ntes los ntawm ib tug detector los ua ib tug spectrum. Nws yog ib qho tseem ceeb heev uas daim ntawv lo fluorescent yog sib xws thiab ruaj khov rau cov tshuaj tiv thaiv tshwj xeeb thiab nws yuav tsum tau muab sib xyaw kom zoo rau cov tshuaj tiv thaiv kom tau txais cov txiaj ntsig raug. Ib qho ntawm feem ntau siv fluorochromes yog fluorescein isothiocyanate (FITC), uas yog xim ntsuab, nrog rau nqus thiab emission ncov wavelengths ntawm 490 nm thiab 520 nm, raws li. Rhodamine, lwm tus neeg siv hauv IF, yog xim liab thiab muaj qhov sib txawv ntawm qhov nqus thiab emission peak wavelengths ntawm 553 nm thiab 627 nm.
Immunohistochemistry (IHC) yog dab tsi?
IHC yog ib txoj hauv kev kuaj molecular xyaum txhawm rau txheeb xyuas thiab paub meej tias muaj cov antigen hauv lub hom phiaj ntawm tes. Lub hom phiaj ntawm tes yuav yog cov kab mob sib kis, kab mob microbial los yog qog nqaij hlav malignant. IHC siv cov tshuaj tiv thaiv kab mob monoclonal thiab polyclonal los txiav txim seb muaj cov antigens nyob rau ntawm lub xov tooj ntawm tes ntawm lub hom phiaj. Cov txheej txheem yog ua raws li antigen-antibody khi. Ib qho kev kuaj pom tau muab sib txuas rau cov tshuaj tiv thaiv kab mob txhawm rau txhawm rau kuaj pom muaj lossis tsis muaj cov tshuaj tiv thaiv tshwj xeeb. Cov cim no tuaj yeem yog cov cim tshuaj xws li enzymes, fluorescently tagged antibodies lossis xov tooj cua sau cov tshuaj tiv thaiv.
Daim duab 02: Nas-hlwb hlais los ntawm Immunohistochemistry
Daim ntawv thov nrov tshaj plaws ntawm IHC yog nyob rau hauv kev mob qog noj ntshav hauv hlwb txhawm rau txheeb xyuas qhov muaj cov qog nqaij hlav, tab sis nws kuj yog siv los kuaj xyuas cov kab mob sib kis.
Dab tsi yog qhov zoo sib xws ntawm Immunofluorescence thiab Immunohistochemistry?
- Immunofluorescence thiab Immunohistochemistry tshwm sim nyob rau hauv vitro mob.
- Ob txoj kev yog ua raws li cov tshuaj tiv thaiv kab mob antigen
- Ob leeg yog cov txheej txheem nrawm heev.
- Cov txiaj ntsig ntawm cov txheej txheem yog rov tsim dua.
- Ob leeg tau txhim kho cov ntaub ntawv zoo.
- Cov tswv yim no siv rau kev kuaj mob qog noj ntshav thiab kis kab mob.
Qhov txawv ntawm Immunofluorescence thiab Immunohistochemistry yog dab tsi?
Immunofluorescence vs Immunohistochemistry |
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| IF yog cov txheej txheem tshawb nrhiav qhov twg cov tshuaj tiv thaiv siv hauv qhov kev ntsuam xyuas tau sau npe siv cov fluorescent dyes lossis fluorescent proteins rau kev kuaj pom. | IHC yog cov txheej txheem tshawb nrhiav qhov twg cov tshuaj tiv thaiv uas siv hauv qhov kev ntsuam xyuas tau sau npe siv tshuaj lossis cov khoom siv hluav taws xob rau kev kuaj pom. |
| Nyob zoo | |
| Qhov tseeb yog siab dua hauv IF txheej txheem piv rau IHC. | Qhov tseeb yog qis dua hauv IHC. |
| Specificity | |
| IF yog qhov tshwj xeeb. | IHC tsawg dua. |
Summary – Immunofluorescence vs Immunohistochemistry
Molecular mechanisms tau coj ntau yam kev hloov pauv hauv kev kho mob, ua rau muaj kev sim tshuaj molecular siab heev uas tau ua rau muaj kev hloov pauv hauv kev kuaj mob. Cov kev tsim kho no tau ua rau muaj kev txheeb xyuas sai thiab raug thiab pom tseeb ntawm tus kab mob, yog li ua kom muaj kev tswj hwm zoo thiab tsim cov tshuaj. Cov tswv yim no kuj tau siv nyob rau hauv pharmacology thiaj li nrhiav tau lub hom phiaj ntawm cov tshuaj thiab kom paub meej tias lub pharmacokinetic zog ntawm cov tshuaj thaum lub sij hawm cov tshuaj metabolism. IF thiab IHC yog ob txoj kev kuaj mob uas yog raws li lub tswv yim ntawm antigen thiab antibody khi, txawm hais tias hom kev kuaj pom hauv ob txoj kev sib txawv. IF siv lub hauv paus ntsiab lus ntawm fluorescence los kuaj xyuas cov antigen thiab IHC siv lub tswv yim ntawm cov tshuaj sib xyaw kom pom cov antigen. Qhov no yog qhov txawv ntawm IF thiab IHC.
Download PDF Version of Immunofluorescence vs Immunohistochemistry
Koj tuaj yeem rub tawm PDF version ntawm kab lus no thiab siv nws rau offline hom phiaj raws li daim ntawv ceeb toom. Thov download PDF version ntawm no Qhov txawv Ntawm Immunofluorescence thiab Immunohistochemistry.
