Qhov Tseem Ceeb - Immunocytochemistry vs Immunohistochemistry
Immunocytochemistry (ICC) thiab Immunohistochemistry (IHC) yog ob txoj kev siv dav hauv kev kuaj mob molecular, uas txheeb xyuas thiab lees paub qhov tshwm sim ntawm ob qho tib si tsis sib kis thiab kab mob sib kis raws li cov cim molecular muaj nyob rau ntawm cov hlwb. Qhov sib txawv tseem ceeb ntawm immunocytochemistry thiab immunohistochemistry yog cov molecule uas yog siv los ua cov txheej txheem tshuaj xyuas hauv cov txheej txheem no. Hauv ICC, thawj thiab theem nrab cov tshuaj tiv thaiv kab mob sib txuas nrog cov cim xws li fluorescence yog siv thaum IHC, monoclonal thiab polyclonal antibodies yog siv rau kev txiav txim siab.
YImmunocytochemistry (ICC) yog dab tsi?
ICC siv cov tshuaj tiv thaiv thawj thiab theem nrab ua ke rau cov cim xws li cov cim fluorescent lossis cov enzymes thiab yog ib txoj hauv kev tshawb pom muaj zog los kuaj pom cov tshuaj tiv thaiv muaj nyob rau ntawm lub hom phiaj hlwb uas tuaj yeem yog cov kab mob sib kis los yog cov qog nqaij hlav cancer. Peb hom kev tswj yuav tsum tau ua rau immunocytochemistry.
- Primary Antibody - tswj uas qhia qhov tshwj xeeb ntawm thawj cov tshuaj tiv thaiv kab mob khi rau cov antigen
- Secondary Antibody - tswj uas qhia tias daim ntawv lo yog tshwj xeeb rau thawj cov tshuaj tiv thaiv
- Cov Ntawv Sau Npe - qhia cov ntawv sau npe yog qhov tshwm sim ntawm daim ntawv sau ntxiv thiab tsis yog qhov tshwm sim ntawm cov ntawv cim endogenous.
Daim duab 01: Immunocytochemistry sau cov proteins hauv cov hlwb (ntawm no, Tyrosine hydroxylase nyob rau hauv cov axons ntawm sympathetic autonomic neurons muaj nyob rau hauv ntsuab).
Cov tshuaj tiv thaiv thawj zaug yog tshwj xeeb rau txhua qhov tshuaj tiv thaiv tshiab thiab tsis tuaj yeem rov ua dua rau txhua qhov kev sim. Cov tshuaj tiv thaiv kab mob thib ob yog tsim los ntawm thawj cov tshuaj tiv thaiv kab mob uas siv hauv kev sim thiab suav nrog txhua qhov kev sim. Kev tswj daim ntawv lo yog suav nrog yog tias muaj kev hloov pauv ntawm tus txheej txheem, cov qauv hloov pauv, lossis thaum pom cov cim tsis tau pom dua.
Ob daim ntawv thov tseem ceeb ntawm ICC yog Xov Tooj Cua Immuno - Kev Tshawb Fawb (RIA) thiab Enzyme Linked Immunosorbent Assay (ELISA). Cov tshuaj tiv thaiv feem ntau siv yog immunoglobulin G.
Immunohistochemistry (IHC) yog dab tsi?
Hauv Immunohistochemistry, cov qauv hauv paus muaj cov tshuaj tiv thaiv kab mob monoclonal thiab polyclonal txhawm rau txiav txim siab muaj cov tshuaj tiv thaiv hauv cov hlwb txawv teb chaws. Cov txheej txheem no yog ua raws li cov tshuaj tiv thaiv tshwj xeeb ntawm antigen-antibody khi. Cov tshuaj tiv thaiv uas siv hauv kev tshawb nrhiav tuaj yeem raug cim nrog cov cim sib txawv; lawv tuaj yeem yog cov cim fluorescence, cov cim hluav taws xob lossis cov cim tshuaj lom neeg. Los ntawm kev ua kom yooj yim hauv vitro khi ntawm cov tshuaj tiv thaiv kab mob thiab cov tshuaj tiv thaiv kab mob, qhov muaj lossis tsis muaj cov protein tshwj xeeb ntawm cov cell tuaj yeem txiav txim siab.
Daim duab 02: Immunohistochemical staining ntawm lub raum ib txwm nrog CD10
Tam sim no, cov kws tshawb fawb tau koom tes hauv kev tsim cov tshuaj tiv thaiv kab mob rau cov tshuaj tiv thaiv tshwj xeeb uas muaj nyob hauv cov hlwb uas tuaj yeem tsim los ua qog nqaij hlav qog hlwb lossis cov tshuaj tiv thaiv muaj nyob hauv cov kab mob xws li HIV.
Dab tsi yog qhov zoo sib xws ntawm Immunocytochemistry thiab Immunohistochemistry?
- Cov lus teb yog qhov tshwj xeeb thiab raug hauv ICC thiab IHC.
- Cov ntawv thov ntawm ICC thiab IHC suav nrog kev kuaj mob qog noj ntshav thiab kis kab mob.
- Cov mob tsis muaj menyuam yuav tsum tau khaws cia hauv ob qho xwm txheej, thiab lawv yuav tsum tau ua hauv vitro
- Ob txoj kev muab cov txiaj ntsig rov tsim dua.
- Ob leeg nrawm.
- xov tooj cua labeling, fluorescence cov txheej txheem yog siv los ua txoj kev tshawb nrhiav hauv ICC thiab IHC.
- Ob leeg yog raws li kev sib koom ua ke ntawm antigen-antibody.
Qhov txawv ntawm Immunocytochemistry thiab Immunohistochemistry yog dab tsi?
Immunocytochemistry (ICC) vs Immunohistochemistry (IHC) |
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| ICC siv cov tshuaj tiv thaiv thawj thiab theem nrab cov cim khi xws li cov cim fluorescent lossis cov enzymes thiab yog ib txoj hauv kev tshawb nrhiav muaj zog los kuaj pom cov tshuaj tiv thaiv muaj nyob rau ntawm lub hom phiaj hlwb. | IHC yog ib txoj hauv kev uas siv cov tshuaj tiv thaiv kab mob monoclonal thiab polyclonal los txiav txim seb muaj cov antigens uas yog cov cim tshwj xeeb protein tso rau ntawm lub xov tooj ntawm tes. |
| Sample Source | |
| Cov qauv muab los ntawm cov ntaub so ntswg uas tau ua tiav hauv cov txheej txheem nyias yog siv hauv ICC. | IHC siv cov qauv uas muaj cov hlwb loj hlob hauv monolayer lossis cov hlwb hauv kev ncua uas tau muab tso rau ntawm tus swb. |
| Sample processing | |
| Hauv ICC, cov hlwb yuav tsum tau nkag mus los pab txhawb kev nkag mus rau cov tshuaj tiv thaiv kab mob mus rau lub hom phiaj intracellular. | Nyob hauv IHC, cov hlwb yog formalin-taw, paraffin-embedded ua ntej staining. |
Summary – Immunocytochemistry vs Immunohistochemistry
Molecular diagnostics yog siv los txheeb xyuas thiab paub meej tias qhov tshwm sim ntawm cov kab mob tsis sib kis thiab cov kab mob sib kis raws li cov cim molecular muaj nyob rau ntawm cov hlwb. Cov cim molecular tuaj yeem yog cov protein lossis cov kab ke ntawm DNA lossis RNA; txoj kev loj hlob ntawm cov thev naus laus zis xws li ICC thiab IHC tau qhib txoj hauv kev rau cov kws tshawb fawb los txheeb xyuas tus kab mob thiab nws qhov ua rau thaum ntxov. Ob leeg ICC thiab IHC nyob ntawm qhov kev cuam tshuam tshwj xeeb ntawm cov tshuaj tiv thaiv kab mob thiab cov tshuaj tiv thaiv kab mob txawm hais tias cov qauv coj mus kuaj. Qhov sib txawv tseem ceeb ntawm immunocytochemistry thiab immunohistochemistry yog cov qauv ua ntawm ob txoj kev.
Download PDF Version of Immunocytochemistry vs Immunohistochemistry
Koj tuaj yeem rub tawm PDF version ntawm kab lus no thiab siv nws rau offline hom phiaj raws li daim ntawv ceeb toom. Thov download PDF version ntawm no Qhov txawv Ntawm Immunocytochemistry thiab Immunohistochemistry.
