Qhov sib txawv tseem ceeb ntawm N glycosylation thiab O glycosylation yog tias N glycosylation tshwm sim hauv asparagine residues whereas O glycosylation tshwm sim nyob rau hauv sab saw ntawm serine lossis threonine residues.
Glycosylation yog kev tswj hwm kev hloov pauv ntawm cov organic molecule xws li cov protein los ntawm kev sib ntxiv ntawm cov piam thaj molecule. Nws yog ib qho tseem ceeb ntawm cov txheej txheem biochemical thiab kev tswj hwm cov txheej txheem ntawm kev ua cov protein ntau hauv cov hlwb.
YN Glycosylation yog dab tsi?
N glycosylation lossis N-txuas glycosylation yog qhov txuas ntawm oligosaccharide qab zib molecule rau nitrogen atom hauv asparagine residue ntawm protein molecule. Cov piam thaj molecule no tseem hu ua glycan. Nws txuas nrog nitrogen atom hauv amide pawg ntawm asparagine residue. Tsis tas li ntawd, txoj kev sib txuas no yog qhov tseem ceeb hauv cov qauv thiab kev ua haujlwm ntawm qee cov protein eukaryotic. Ntxiv mus, cov txheej txheem no tshwm sim hauv eukaryotes, dav hauv archaea, thiab tsis tshua muaj kab mob.
Kev txuas ntawm glycan residue rau cov protein yuav tsum tau lees paub ntawm qhov kev pom zoo ua ntu zus. Piv txwv li, N-txuas glycans yuav luag ib txwm txuas nrog nitrogen atom ntawm asparagine sab saw uas tshwm sim ua ib feem ntawm Asn-X-Ser / Thr kev pom zoo ua ntu zus. Ntawm no, X yog ib qho amino acid tshwj tsis yog proline (Pro). N-txuas glycans muaj ob qho tib si hauv nruab nrab thiab sab nraud ua haujlwm.
Daim duab 01: Hom N-Glycans
Muaj cov kev kho mob tseem ceeb ntawm N glycosylation txheej txheem. Piv txwv li, nws muaj feem xyuam nrog cov kab mob sib txawv, suav nrog mob caj dab rheumatoid, mob ntshav qab zib hom 1, Crohn's kab mob, thiab mob qog noj ntshav. Ntxiv mus, ntau cov tshuaj kho cov proteins hauv khw yog cov tshuaj tiv thaiv uas yog N-txuas glycoproteins.
YO Glycosylation yog dab tsi?
O glycosylation lossis O-linked glycosylation yog qhov txuas ntawm cov piam thaj molecule rau oxygen atom ntawm serine lossis threonine residues hauv cov protein molecule. Cov txheej txheem no yog kev hloov pauv tom qab hloov pauv uas tshwm sim tom qab kev sib txuas ntawm cov protein. Cov txheej txheem no tuaj yeem tshwm sim hauv ob qho tib si eukaryotes thiab prokaryotes. E.g. Hauv eukaryotes, N glycosylation tshwm sim hauv endoplasmic reticulum, Golgi apparatus thiab qee zaum hauv cytoplasm thaum nyob hauv prokaryotes, nws tshwm sim hauv cytoplasm.
Daim duab 02: Ntxiv ntawm Ribitol Qab Zib
Thaum O glycosylation txheej txheem, ntau cov suab thaj tuaj yeem ntxiv rau cov serine lossis threonine, thiab qhov sib ntxiv no tuaj yeem cuam tshuam rau cov protein ntau los ntawm kev hloov pauv cov protein ruaj khov thiab los ntawm kev tswj hwm cov haujlwm protein.
Qhov txawv ntawm N Glycosylation thiab O Glycosylation yog dab tsi?
N glycosylation thiab O glycosylation yog cov txheej txheem biochemical tseem ceeb. Qhov sib txawv tseem ceeb ntawm N glycosylation thiab O glycosylation yog tias N glycosylation tshwm sim hauv asparagine residues thaum O glycosylation tshwm sim hauv cov saw hlau ntawm serine lossis threonine residues. Feem ntau, N glycosylation tshwm sim hauv cov kab mob eukaryotic thiab hauv archaea thaum O glycosylation tshwm sim hauv cov kab mob prokaryotic nkaus xwb.
Hauv qab no yog cov lus qhia luv luv ntawm qhov sib txawv ntawm N glycosylation thiab O glycosylation.
Summary – N Glycosylation vs O Glycosylation
Hauv luv luv, N glycosylation lossis N-txuas glycosylation yog qhov txuas ntawm oligosaccharide qab zib molecule rau nitrogen atom hauv asparagine residue ntawm cov protein molecule. O glycosylation lossis O-linked glycosylation yog qhov txuas ntawm cov piam thaj molecule rau cov pa oxygen atom ntawm serine lossis threonine residues hauv cov protein molecule. Yog li ntawd, qhov sib txawv tseem ceeb ntawm N glycosylation thiab O glycosylation yog tias N glycosylation tshwm sim hauv asparagine residues, whereas O glycosylation tshwm sim nyob rau hauv cov saw ntawm serine los yog threonine residues.
