Cov ntsiab lus sib txawv ntawm cov sib txawv thiab qhov sib txawv ntawm cov khoom uas cais tawm ntawm qhov sib xyaw kom sib xyaw ua ke nyob rau hauv cov dej sib xyaw ua ke raws li qhov ntom ntom.
Centrifugation yog txoj kev sib cais ntawm cov khoom sib txawv hauv qhov sib xyaw ua ke. Txoj kev no suav nrog kev sib hloov ntawm cov qauv nyob ib ncig ntawm lub axis ruaj khov, uas ua rau kev tsim cov centrifugal quab yuam. Lub zog centrifugal ua rau cov khoom hauv cov qauv txav mus los ntawm cov kua nruab nrab. Cov txheej txheem no ua rau cov sedimentation ntawm cov khoom los yog cov hlwb nrog ntau qhov sib txawv thiab qhov ntom ntom ntawm tus nqi sib txawv.
Dab tsi yog qhov sib txawv centrifugation?
Differential centrifugation yog cov txheej txheem tshuaj ntsuam xyuas uas peb tuaj yeem cais cov khoom sib xyaw ua ke nyob ntawm qhov loj ntawm cov khoom. Nws yog daim ntawv yooj yim tshaj plaws ntawm centrifugation thiab tseem hu ua txawv pelleting. Txoj kev no yog qhov tseem ceeb hauv kev sib cais cov khoom hauv ib lub cell. Cov khoom uas muaj qhov sib txawv ntawm qhov sib txawv ntawm qhov sib txawv ntawm qhov sib txawv ntawm qhov centrifugation. Hauv lwm lo lus, cov khoom loj loj sediment sai dua cov khoom me me. Ntxiv mus, tus nqi sedimentation tuaj yeem nce ntxiv los ntawm kev ua kom lub zog centrifugal.
Thaum xav txog nws cov ntawv thov, qhov sib txawv centrifugation yog qhov muaj txiaj ntsig zoo hauv kev sau cov hlwb lossis tsim cov khoom siv tsis zoo los ntawm cov ntaub so ntswg homogenate. E.g. Lub siab homogenate muaj nuclei, mitochondria, lysosomes, thiab daim nyias nyias vesicles. Yog hais tias peb centrifuge no homogenate ntawm ib tug tsawg ceev thiab ib tug luv luv lub sij hawm, peb yuav tau txais lub loj nuclei raws li lub pellet. Yog tias peb siv lub zog centrifugal siab, ces peb tuaj yeem tau txais mitochondria hauv pellet. Txawm li cas los xij, cov qauv tshuaj lom neeg ib txwm muaj kev sib kis.
Density Gradient Centrifugation yog dab tsi?
Density gradient centrifugation yog ib qho kev tshuaj ntsuam xyuas uas peb tuaj yeem cais cov khoom hauv qhov sib xyaw ua ke raws li qhov ntom ntawm cov khoom. Nyob rau hauv txoj kev no, cov tshuaj yog concentrated nyob rau hauv ib tug daws ntawm cesium ntsev los yog nyob rau hauv sucrose. Txoj kev muaj feem cuam tshuam ntawm cov khoom raws li buoyancy ceev. Qhov ceev gradient nyob rau hauv txoj kev no yog Cesium ntsev los yog sucrose nruab nrab. Muaj ob hom kev ceev gradient centrifugation: tus nqi-zonla centrifugation thiab isopycnic centrifugation.
Tus nqi-zonal centrifugation cuam tshuam nrog kev sib cais tawm uas muaj cov txheej txheem txheej nrog ib cheeb tsam nqaim nyob rau sab saum toj ntawm qhov ceev gradient. Hauv cov txheej txheem no, cov khoom yuav txav mus los ntawm cov nqi sib txawv raws li lub zog centrifugal nyob ntawm qhov ntom ntawm cov khoom. Feem ntau, tag nrho cov khoom tsim pellets vim qhov ntom ntawm cov khoom yog siab dua qhov ntom ntom ntom.
Isopycnic centrifugation yog hom thib ob ntawm kev ceev gradient centrifugation txoj kev. Nws pib nrog homogeneous tov. Nyob rau hauv lub centrifugal quab yuam, cov hais nyob rau hauv lub analyte sib tov txav mus txog rau thaum lub ceev ntawm cov hais zoo ib yam li qhov ceev gradient. Yog li ntawd, peb tuaj yeem hu cov txheej txheem no ua equilibrium centrifugation thiab.
Dab tsi yog qhov txawv ntawm qhov sib txawv thiab qhov ntom ntom ntom nti centrifugation?
Differential and density gradient centrifugation yog ob hom txheej txheem centrifugation koom nrog kev sib cais ntawm cov khoom hauv ib qho kev sib xyaw ua ke. Tus yuam sij sib txawv ntawm cov sib txawv thiab qhov sib txawv ntawm Centrifugation cais cov khoom uas cais tawm ntawm qhov sib xyaw ua ke raws li qhov ntom ntom.
Hauv qab infographic tabulates ntau qhov sib txawv ntawm qhov sib txawv thiab qhov ntom ntom gradient centrifugation.
Summary – Differential vs Density Gradient centrifugation
Differential and density gradient centrifugation yog ob hom txheej txheem centrifugation koom nrog kev sib cais ntawm cov khoom hauv ib qho kev sib xyaw ua ke. Tus yuam sij sib txawv ntawm cov sib txawv thiab qhov sib txawv ntawm Centrifugation cais cov khoom uas cais tawm ntawm qhov sib xyaw ua ke raws li qhov ntom ntom.
