Qhov sib txawv tseem ceeb ntawm chimeric thiab humanized antibody yog tias chimeric antibody yog cov tshuaj tiv thaiv kab mob uas ua los ntawm ntau hom tsiaj, thiab nws nqa ntau dua ntawm cov protein tsis yog tib neeg thaum tib neeg cov tshuaj tiv thaiv kab mob yog cov tshuaj tiv thaiv kab mob tsim los ntawm cov protein hloov pauv. kab mob sib kis tsis yog tib neeg.
Chimeric thiab humanized antibodies yog ob hom tshuaj tiv thaiv tsis yog tib neeg. Lawv raug tsim los ua cov tshuaj tiv thaiv kab mob hauv cov chaw kuaj mob. Thaum muab piv rau humanized antibodies thiab chimeric antibodies, humanized antibodies muaj tsawg dua kev pheej hmoo ntawm immunogenicity dua li chimeric antibodies. Hauv cov tshuaj tiv thaiv chimeric, cov cheeb tsam tas mus li nas tau hloov los ntawm tib neeg cov cheeb tsam tas li. Nyob rau hauv humanized antibodies, nas amino acids nyob rau hauv complementarity-determining regions (CDRs) yog kis mus rau hauv tib neeg V-thav duab cheeb tsam.
Chimeric Antibody yog dab tsi?
Chimeric antibody yog ib qho tshuaj tiv thaiv tsis yog tib neeg uas yog tsim los ntawm cov npe los ntawm ntau hom. Piv txwv li, thaj tsam Fc ntawm tus nas mAb tuaj yeem hloov tau los ntawm cov cheeb tsam ntawm tib neeg cov tshuaj tiv thaiv kab mob lossis lwm hom tshuaj tiv thaiv kab mob. Chimeric cov tshuaj tiv thaiv uas muaj tib neeg qhov chaw tas li thiab cov nas sib txawv ntawm tus nas yog pheej yig dua li humanized antibodies.
Daim duab 01: Chimeric thiab Humanized Antibodies
Chimeric cov tshuaj tiv thaiv khaws cia cov tshuaj tiv thaiv thawj tus antigen tshwj xeeb thiab kev sib raug zoo. Li no, lawv yog cov cuab yeej tseem ceeb rau kev tshawb fawb hauv vitro thiab hauv vivo thiab kev txhim kho kev kuaj mob. Infliximab, rituximab thiab abciximab yog ob peb yam piv txwv ntawm chimeric antibodies. Infliximab los yog Remicade yog siv los kho rheumatoid mob caj dab. Rituximab lossis Rituxan yog siv los kho mob qog noj ntshav.
Dab tsi yog Humanized Antibody?
Humanization yog ib kauj ruam tseem ceeb hauv kev tsim cov tshuaj tiv thaiv kab mob los ntawm cov khoom tsis yog tib neeg. Cov txheej txheem no cuam tshuam txog kev hloov lossis grafting ntawm qhov tseem ceeb tsis yog tib neeg cov amino acids mus rau tib neeg cov tshuaj tiv thaiv kab mob. Feem ntau, nas cov amino acids nyob rau hauv complementarity-determining regions (CDRs) yog pauv mus rau hauv tib neeg V-thav duab cheeb tsam. Qhov tshwm sim antibody yog hu ua humanized antibody. Thaum tsim tawm, tib neeg cov ntsiab lus tau qhia ntau npaum li qhov ua tau txhawm rau txo qhov kev pheej hmoo ntawm immunogenicity. Tab sis nws kuj qhia txog cov ntsiab lus tsis yog tib neeg txaus kom tswj tau qhov kev sib khi qub ntawm niam txiv cov tshuaj tiv thaiv. Yog li ntawd, humanized antibodies yog ib nrab nas Ig thiab ib nrab tib neeg Ig.
Humanized monoclonal antibodies yog siv los kho ntau yam mob, suav nrog mob qog noj ntshav. Humanization ntawm cov tshuaj tiv thaiv zoo heev txo cov peev xwm immunogenic hauv vivo piv rau cov tshuaj tiv thaiv chimeric. Trastuzumab (Herceptin) yog thawj cov tshuaj tiv thaiv tib neeg tsim los kho mob qog noj ntshav.
Dab tsi yog qhov zoo sib xws ntawm Chimeric thiab Humanized Antibody?
- Chimeric thiab humanized antibodies yog cov tshuaj tiv thaiv tsis yog tib neeg.
- Lawv yog los ntawm tsis yog tib neeg.
- Ob leeg muaj cov protein ntau ua kom zoo ib yam li tib neeg cov tshuaj tiv thaiv.
- Lawv yog cov tshuaj tiv thaiv kab mob monoclonal uas tsim los ntawm nas lossis nas.
- Lawv yog cov tshuaj tiv thaiv kab mob.
- Ntxiv mus, lawv tau siv los kho ntau yam mob, suav nrog mob qog noj ntshav.
- Cov tshuaj tiv thaiv kab mob no tuaj yeem siv los ua kev tswj hwm rau kev txheeb xyuas cov tshuaj lead ua ntej thiab cov tshuaj muaj zog rau kev txhim kho cov tshuaj kho tshiab.
Qhov txawv ntawm Chimeric thiab Humanized Antibody yog dab tsi?
Chimeric antibody yog ib qho tshuaj tiv thaiv uas muaj nws cov thawj antigen-binding variable domains nrog rau qhov chaw tas li los ntawm ntau hom. Humanized antibody, ntawm qhov tod tes, yog ib qho tshuaj tiv thaiv kab mob los ntawm cov tsiaj tsis yog tib neeg uas nws cov protein ntau raug hloov kho kom lawv zoo sib xws rau cov tshuaj tiv thaiv kab mob uas tsim los ntawm tib neeg. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm chimeric thiab humanized antibody.
Tsis tas li ntawd, cov tshuaj tiv thaiv chimeric nqa ntau dua ntawm cov proteins uas tsis yog tib neeg lub cev thaum tib neeg cov tshuaj tiv thaiv tsis muaj cov protein ntau dua uas tsis yog tib neeg.
Hauv qab inforgrphic qhia ntau qhov sib txawv ntawm chimeric thiab humanized antibody.
Summary – Chimeric vs Humanized Antibody
Kev siv cov tshuaj tiv thaiv kab mob, tshwj xeeb tshaj yog cov tshuaj tiv thaiv kab mob monoclonal, raws li kev kho mob muaj keeb kwm ntev. Chimeric thiab humanized antibodies yog kev kho mob monoclonal antibodies muab los ntawm cov khoom tsis yog tib neeg. Chimeric antibodies yog tsim los ntawm kev hloov cov murine tas li ntawm tib neeg thaj chaw. Humanized antibodies yog tsim los ntawm kev qhia cov nas ntxiv-txiav txim siab thaj tsam (CDRs) rau hauv tib neeg V-thaj tsam cheeb tsam. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm chimeric thiab humanized antibody. Thaum piv rau humanized antibody, chimeric antibody muaj ib tug loj stretch ntawm cov protein uas tsis yog tib neeg.
