Qhov sib txawv tseem ceeb ntawm acidophilic thiab basophilic yog cov khoom siv acidophilic ntawm lub xov tooj ntawm tes yog cov kua qaub-hlub thiab acidic dyes yog siv los stain lawv thaum basophilic Cheebtsam ntawm lub xovtooj yog lub hauv paus-hlub thiab cov xim yooj yim yog siv los stain lawv.
Staining yog txheej txheem siv los pom cov hlwb thiab lawv cov khoom vim tias ntau lub hlwb tsis muaj xim thiab pob tshab. Qee cov khoom ntawm tes yog acid-hlub, thaum qee qhov tseem ceeb-hlub. Acid dyes thiab yooj yim dyes yog ob hom dyes uas nquag siv hauv cov txheej txheem qhia. Basophilic staining siv cov xim yooj yim thaum acidophilic staining siv acidic dyes. Yog li ntawd, acidophilic los yog acid-hlub Cheebtsam khi nrog acidic dyes thaum basophilic los yog lub hauv paus hlub Cheebtsam khi nrog yooj yim dyes.
Acidophilic yog dab tsi?
Acidophilic yog ib lo lus siv los hais txog cov kua qaub-hlub cov khoom hauv lub cell. Qhov tseeb, cov kua qaub-hlub Cheebtsam yog cationic (zoo them) lossis cov khoom siv hauv cov hlwb. Cytoplasmic proteins yog ib qho piv txwv ntawm acidophilic Cheebtsam. Proteins raug nqi zoo ntawm pH siab dua; Yog li ntawd lawv yog acidophilic. Ntau cov proteins yog acidophilic ntawm physiological pH.
Daim duab 01: Staining of Cells with Basic and Acid Dye
Thaum peb ntxiv ib qho acidic stains, acidic stains teb nrog acidophilic Cheebtsam ntawm tes thiab pom lawv. Eosin, Txiv kab ntxwv G, thiab kua qaub fuschin yog qee cov kua qaub dyes.
Basophilic yog dab tsi?
Basophilic Cheebtsam ntawm cov hlwb yog lub hauv paus-hlub qhov chaw ntawm lub cell. Qhov tseeb, lawv yog anionic (tsis zoo them) lossis acidic Cheebtsam hauv cov hlwb. Lawv nyiam cov xim yooj yim. Qee qhov piv txwv ntawm basophilic Cheebtsam yog nucleic acids. Txij li cov nucleic acids muaj phosphate pawg, lawv raug nqi tsis zoo thiab nyiam ntawm cov xim yooj yim. Ntxiv mus, proteoglycans yog basophilic vim lawv cov suab thaj thiab esterified sulfates. Thaum peb ntxiv cov xim yooj yim, cov khoom siv basophilic ntawm cov hlwb hnov mob nrog cov xim yooj yim thiab yog xim los ntawm lawv. Ib qho piv txwv ntawm cov xim yooj yim yog hematoxylin. Methylene xiav, alcian xiav thiab toluidine xiav yog ob peb lwm yam dyes.
Dab tsi zoo sib xws ntawm Acidophilic thiab Basophilic?
- Ib lub cell muaj ob qho tib si acidophilic thiab basophilic Cheebtsam.
- Lawv stained nrog lawv cov dyes.
- Ob qho tib si acidophilic thiab basophilic tshuaj yog them cov khoom.
Qhov txawv ntawm Acidophilic thiab Basophilic yog dab tsi?
Acidophilic Cheebtsam ntawm lub xovtooj tau raug them zoo, thaum cov khoom basophilic ntawm lub xovtooj raug them tsis zoo. Yog li ntawd, acidophilic Cheebtsam yog attracted rau acidic dyes thaum basophilic tshuaj yog attracted rau cov yooj yim dyes. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm acidophilic thiab basophilic. Cytoplasmic proteins yog acidophilic thaum nucleic acids yog basophilic. Tsis tas li ntawd, eosin yog ib qho acidic dye uas stains acidophilic tshuaj thaum haematoxylin yog ib qho yooj yim dye uas stains basophilic tshuaj.
Cov infographic hauv qab no nthuav tawm qhov sib txawv ntawm acidophilic thiab basophilic hauv daim ntawv teev npe.
Summary – Acidophilic vs Basophilic
Acidophilic tshuaj yog acid-hlub Cheebtsam ntawm cell. Yog li ntawd, lawv tuaj yeem stained nrog acidic dye. Ntxiv mus, lawv raug nqi zoo. Nyob rau hauv sib piv, basophilic tshuaj yog lub hauv paus-hlub Cheebtsam ntawm lub hlwb. Lawv tuaj yeem stained nrog cov xim yooj yim. Basophilic Cheebtsam yog qhov tsis zoo them nqi. Ntau cov cytoplasmic proteins yog acidophilic thaum nucleic acids yog basophilic. Yog li, qhov no yog cov ntsiab lus ntawm qhov sib txawv ntawm acidophilic thiab basophilic.
