Qhov sib txawv tseem ceeb ntawm electrophoresis thiab dielectrophoresis yog tias electrophoresis cais cov khoom siv hluav taws xob, hos dielectrophoresis cais cov khoom tsis them lossis tsis them.
Electrophoresis thiab dielectrophoresis yog cov txheej txheem kev tshuaj xyuas tseem ceeb hauv kev ua haujlwm ntawm biochemistry. Cov no yog txoj kev sib cais peb tuaj yeem siv los cais cov khoom xav tau los ntawm kev sib xyaw ntawm cov khoom.
Electrophoresis yog dab tsi?
Electrophoresis yog cov txheej txheem kev tshuaj xyuas uas muaj txiaj ntsig zoo rau kev tshuaj xyuas tus qauv siv cov khoom hluav taws xob ntawm cov tshuaj muaj nyob hauv cov qauv ntawd. Ntawm no, peb tuaj yeem soj ntsuam cov lus tsa suab ntawm dispersed solute nyob rau hauv nruab nrab tsom xam. Yog li, peb tuaj yeem txiav txim siab qhov kev txav ntawm cov tshuaj lom neeg txheeb ze rau nruab nrab.
Daim duab 01: Gel Electrophoresis Technique
Txawm li cas los xij, cov txheej txheem no yuav tsum tau tsim qee yam tshwj xeeb. Piv txwv li, peb yuav tsum muab qhov nruab nrab nrog kev cuam tshuam los ntawm qhov chaw hluav taws xob tsis sib xws. Txoj kev xav tom qab cov txheej txheem no yog qhov sib txawv ntawm qhov sib txawv ntawm qhov nruab nrab ntawm kev txav mus los ntawm qhov sib txawv ntawm kev tsiv teb tsaws chaw nyob rau hauv lub xub ntiag ntawm hluav taws xob teb.
Lwm lo lus rau electrophoresis yog "electrokinetic phenomena". Nyob ntawm seb hom ion tam sim no nyob rau hauv cov qauv, cov txheej txheem ntawm electrophoresis muaj ob pawg xws li cataphoresis thiab anaphoresis.
Cataphoresis yog electrophoresis ntawm cations (positively charged ions) thaum anaphoresis yog electrophoresis ntawm anions (tsis zoo them ions). Daim ntawv thov tseem ceeb tshaj plaws ntawm electrophoresis yog nyob rau hauv extraction ntawm DNA tawg raws li nws loj.
YDielectrophoresis yog dab tsi?
Dielectrophoresis yog cov txheej txheem kev tshuaj xyuas uas lub zog tau tawm ntawm cov khoom siv hluav taws xob thaum cov khoom nyob rau hauv qhov chaw tsis muaj hluav taws xob. Hauv cov txheej txheem no, cov khoom tsis tas yuav tsum tau them nyiaj txhawm rau txhawm rau cais lawv. Txawm li cas los xij, lub zog ntawm kev quab yuam tawm ntawm dielectric particle nyob ntawm hom nruab nrab, cov khoom hluav taws xob ntawm cov khoom, cov duab thiab qhov loj ntawm cov khoom.
Daim duab 02: Txoj Kev Xav Tom Qab Dielectrophoresis Technique
Dielectrophoresis tso cai rau kev sib cais ntawm cov hlwb, kev taw qhia thiab kev tswj hwm ntawm nanoparticles, thiab lwm yam. Cov hlwb muaj dielectric zog. Yog li ntawd, cov txheej txheem no muaj ntau yam kev siv hauv kev kho mob. Piv txwv li, peb tuaj yeem siv nws los cais cov qog nqaij hlav cancer ntawm cov hlwb noj qab haus huv. Tsis tas li ntawd, platelets tuaj yeem sib cais los ntawm lwm cov qe ntshav. Tsis tas li ntawd, Dielectrophoresis yog qhov tseem ceeb hauv kev tsim khoom semiconductor.
Qhov txawv ntawm Electrophoresis thiab Dielectrophoresis yog dab tsi?
Electrophoresis thiab dielectrophoresis yog cov txheej txheem kev tshuaj xyuas tseem ceeb hauv kev ua haujlwm ntawm biochemistry. Electrophoresis yog cov txheej txheem kev tshuaj ntsuam xyuas uas muaj txiaj ntsig zoo rau kev tshuaj xyuas cov qauv siv cov khoom hluav taws xob ntawm cov tshuaj uas muaj nyob hauv cov qauv ntawd. Nyob rau hauv sib piv, dielectrophoresis yog ib qho kev soj ntsuam cov txheej txheem nyob rau hauv uas ib tug quab yuam yog exerted rau dielectric hais thaum cov hais nyob rau hauv ib tug tsis-uniform hluav taws xob teb. Qhov sib txawv tseem ceeb ntawm electrophoresis thiab dielectrophoresis yog tias electrophoresis cais cov khoom siv hluav taws xob, hos dielectrophoresis cais cov khoom siv los yog tsis them nyiaj.
Tseem muaj qhov sib txawv theoretical ntawm electrophoresis thiab dielectrophoresis. Qhov ntawd yog; cov theories siv hauv cov txheej txheem no txawv. Hauv electrophoresis, cov khoom them nqi txav mus rau qhov sib txawv ntawm qhov hluav taws xob qhov hluav taws xob qhov twg qhov kev tsiv teb tsaws ntawm cov khoom no nyob ntawm hom nruab nrab thiab qhov loj ntawm cov khoom txav. Txawm li cas los xij, electrophoresis, cov khoom txav mus rau hauv nruab nrab hauv cov nyhuv dielectric.
Summary – Electrophoresis vs Dielectrophoresis
Electrophoresis thiab dielectrophoresis yog cov txheej txheem kev tshuaj xyuas tseem ceeb hauv kev ua haujlwm ntawm biochemistry. Qhov sib txawv tseem ceeb ntawm electrophoresis thiab dielectrophoresis yog tias electrophoresis cais cov khoom siv hluav taws xob, hos dielectrophoresis cais cov khoom tsis them los yog tsis them.
