Qhov Tseem Ceeb Qhov txawv - Microarray vs Next Generation Sequencing
DNA sequencing txheej txheem yog siv dav hauv kev lag luam biotechnology, virology, kev kuaj mob thiab kev tshawb fawb forensic. Nws yog cov txheej txheem uas txiav txim siab qhov tseeb ntawm nucleotides, adenine, guanine, thymine thiab cytosine tam sim no nyob rau hauv DNA molecule. DNA sequencing cov txheej txheem tau dhau los ua qhov nrawm rau kev tshawb pom txuj ci tseem ceeb hauv kev tshawb fawb kho mob thiab tshuaj lom neeg. Cov txheej txheem sequencing no tau hloov zuj zus mus rau qhov ua tiav genome ntawm tus kheej cov kab mob xws li tib neeg thiab lwm hom tsiaj. Microarrays thiab Next Generation Sequencing yog cov txheej txheem DNA sequencing niaj hnub. Microarray txheej txheem yog tshwj xeeb raws li hybridization uas muaj cov txheej txheem paub lub hom phiaj. Cov tiam tom ntej sequencing yog raws li kev sib txuas (uas siv DNA polymerase los koom nrog nucleotides) thiab muaj peev xwm ua kom tiav tag nrho cov genome ywj siab ntawm cov hom phiaj yav dhau los. Qhov no yog qhov sib txawv tseem ceeb ntawm Microarray thiab Next Generation Sequencing.
Microarray yog dab tsi?
DNA microarray yog siv los ua cov cuab yeej kuaj xyuas txhawm rau txheeb xyuas ntau txhiab tus kab mob sib txawv tib lub sijhawm. Nws yog ib qho khoom nto, piv txwv li, lub tshuab tsom iav swb, uas muaj cov ntawv sau ntawm microscopic DNA me ntsis luam tawm ntawm nws. Txhua qhov chaw luam tawm muaj cov kab ke paub los yog cov noob. Cov kev sojntsuam paub no tau luam tawm ntawm cov swb ua haujlwm raws li kev sojntsuam txhawm rau txhawm rau txhawm rau txheeb xyuas cov kabmob. Qhov no yog hu ua transcriptome. Hybridization ntawm ob DNA strands yog lub hauv paus ntsiab lus microarrays raws li. Nws yog lub hauv paus ua ke ntawm nucleic acid sequences nrog kev tsim ntawm hydrogen bonds.
Daim duab 01: Microarray
Thaum pib, mRNA molecules tau sau los ntawm cov qauv kev sim thiab cov qauv siv tau los ntawm tus neeg noj qab haus huv. Cov qauv sim tau txais los ntawm cov neeg mob; Piv txwv li, ib tug neeg mob cancer. Thaum tau txais, ob qho qauv mRNA raug hloov mus rau cDNA (ua tiav DNA). Tom ntej no, txhua tus qauv raug sau npe siv lub fluorescent sojntsuam. Cov fluorescent probes yog cov xim sib txawv kom paub qhov txawv ntawm cov qauv cDNA los ntawm kev siv cDNA. Txhawm rau pib qhov kev khi ntawm cDNA molecules rau microarray swb, ob qho qauv sib xyaw ua ke. Hybridization yog txheej txheem uas cov cDNA molecules tau txuas rau DNA sojntsuam ntawm microarray swb. Thaum hybridization tiav lawm, muaj ntau qhov kev tshwm sim tshwm sim txhawm rau txheeb xyuas thiab ntsuas qhov qhia ntawm txhua tus noob nrog cov tsos ntawm cov xim sib txawv raws li tus nqi ntawm cov noob tau hais. Cov txiaj ntsig los ntawm microarray yog siv rau hauv kev tsim cov noob qhia profile uas tuaj yeem siv los txheeb xyuas cov kab mob sib txawv.
Tam sim no Sequencing yog dab tsi?
Next Generation Sequencing (NGS) yog ib txoj hauv kev zoo tshaj plaws ntawm kev sib txuas caj ces. Nws lub hauv paus ntsiab lus zoo ib yam li Sanger Sequencing, nyob ntawm capillary electrophoresis. Hauv NGS, genomic strand yog fragmented thiab ligated rau ib tug template strand. Lub hauv paus ntawm txhua strand raug txheeb xyuas los ntawm cov teeb liab tawm thaum nws txoj kev ligation. Nyob rau hauv Sanger sequencing txoj kev, peb cais cov kauj ruam, sequencing, sib cais, thiab nrhiav kom tau. Raws li cov kauj ruam sib cais no, automation ntawm cov qauv kev npaj yog txwv nyob rau hauv throughput. Hauv NGS, cov txheej txheem yog tsim los siv array raws li kev sib txuas nrog kev sib txuas ntawm cov kauj ruam ntawm Sanger sequencing txheej txheem uas tuaj yeem ua rau ntau lab ntawm cov tshuaj tiv thaiv series yuav tsum tau ua tib lub sijhawm; qhov no ua rau muaj kev kub ceev thiab hla dhau ntawm tus nqi qis.
Daim duab 02: Kev Txhim Kho hauv NGS
NGS yog tsim los ntawm peb kauj ruam; kev npaj cov tsev qiv ntawv (tsim cov tsev qiv ntawv nrog kev siv cov khoom sib cais ntawm DNA), kev nthuav dav (kev nthuav dav ntawm lub tsev qiv ntawv siv clonal amplification thiab PCR) thiab sequencing. Cov txheej txheem genome sequencing uas tau ua rau lub sijhawm ntev heev uas siv Sanger sequencing txheej txheem tuaj yeem ua tiav hauv ob peb teev siv NGS.
Dab tsi yog qhov zoo sib xws ntawm Microarray thiab Lwm Tiam Tiam Sequencing?
Ob qho tib si Microarray thiab Tom ntej no Sequencing yog tsim los siv array raws li sequencing
Qhov txawv ntawm Microarray thiab Lwm Tiam Sequencing yog dab tsi?
Microarray vs Next Generation Sequencing |
|
| Microarray yog ib qho kev sau ntawm microscopic DNA me ntsis txuas rau ntawm cov khoom nto, uas yog siv los ntsuas qhov qhia theem ntawm ntau cov noob ib txhij. | NGS (Tam sim no sequencing) yog ib qho uas tsis yog-Sanger-based high-throughput DNA sequencing thev naus laus zis uas pab txhawb ntau lab lossis ntau txhiab tus DNA strands kom ua raws li qhov sib npaug. |
| Kev sib cuam tshuam nrog Antigen | |
| Microarray yog raws li hybridization uas yog tsim los ntawm cov phiaj xwm paub. | NGS yog raws li kev sib txuas uas siv DNA polymerase los koom nrog nucleotides thiab tsis muaj kev ywj pheej ntawm cov hom phiaj yav dhau los. |
Summary – Microarray vs Next Generation Sequencing
Hauv cov ntsiab lus ntawm kev tshawb fawb, DNA sequencing tau dhau los ua qhov tseem ceeb nrawm. Nws yog dav siv hauv biotechnology, kuaj mob, thiab kev tshawb fawb forensic. Nws tau evolved thiab tsim mus rau ntau npaum thiab ceev sequencing txheej txheem. Microarrays thiab NGS yog ob txoj kev tshawb fawb DNA qib siab tam sim no. Ob leeg yog tsim los siv array raws sequencing. Cov txheej txheem Microarray tso siab rau hybridization thaum NGS yog raws li kev sib txuas, uas siv DNA polymerase los koom nrog nucleotides. Qhov no yog qhov txawv ntawm Microarray thiab Next Generation Sequencing.
Download PDF Version of Microarray vs Next Generation Sequencing
Koj tuaj yeem rub tawm PDF version ntawm kab lus no thiab siv nws rau offline lub hom phiaj raws li daim ntawv ceeb toom. Thov download PDF version ntawm no Qhov txawv Ntawm Microarray thiab Next Generation Sequencing
