Qhov sib txawv tseem ceeb ntawm agar thiab agarose yog tias agar yog cov tshuaj gelatinous tau los ntawm liab algae thaum agarose yog cov kab polymer purified los ntawm agar lossis liab seaweeds.
Agar thiab agarose yog ob hom khoom polysaccharide uas los ntawm cov algae liab lossis seaweed. Lawv muaj txiaj ntsig zoo hauv ntau thaj chaw, xws li chav ua noj, xws li zaub mov, mus rau chemistry labs, ua kab lis kev cai rau kev loj hlob ntawm cov kab mob. Yog li, kev cog qoob loo ntawm cov peev txheej no yog kev lag luam muaj txiaj ntsig, thiab nws tau ua tiav hauv thaj chaw Asia thiab Tebchaws Meskas. Structureally, agarose yog ib tug linear polymer uas muaj alternating D-galactose thiab 3, 6-anhydro-L-galactose units. Ntawm qhov tod tes, agar yog sib xyaw ntawm agarose thiab agaropectin.
Agar yog dab tsi?
Agar, lossis agar-agar, yog polysaccharide rho tawm los ntawm cov algae liab xws li Gracilaria thiab Gelidium. Nws feem ntau yog ib yam khoom gelatinous. Feem ntau nws ua haujlwm raws li lub hauv paus hauv kev npaj cov xov xwm loj hlob rau culturing kab mob, fungi thiab lwm yam microbes rau kev tshawb fawb thiab tshuaj. Agar muaj galactose; ib lub polymer uas tuaj yeem siv los ua cov khoom noj zoo li gelatine uas cov vegans tuaj yeem hloov cov nqaij.
Daim duab 01: Agar
Tsis tas li ntawd, agar muaj agaropectin; ib tug heterogeneous sib tov ntawm me me molecules. Ntawm ob lub ntsiab lus; agarose thiab agaropectin, agarose tso nyiaj rau ntau tshaj 70% ntawm qhov sib tov. Agar melts ntawm kwv yees li 80 0 C thaum nws solidifies qis dua 40 0 C. Yog li, qhov tshwj xeeb no tau ua rau agar rau hauv tus neeg sawv cev tsim nyog hauv microbiological media.
Agarose yog dab tsi?
Agarose yog polysaccharide purified los ntawm agar los yog tau los ntawm agar ua liab seaweed. Nws yog ib tug linear polymer ntawm agarobiose uas yog ib tug disaccharide ua los ntawm D-galactose thiab 3, 6-anhydro-L-galactopyranose.
Daim duab 02: Agarose
Ntxiv mus, agarose yog qhov tseem ceeb ntawm agar uas suav txog ntau dua 70% ntawm nws. Agarose muaj txiaj ntsig zoo hauv kev tsim cov kab mob. Tsis tas li ntawd, agarose yog cov khoom muaj txiaj ntsig hauv kev npaj cov gels rau agarose gel electrophoresis kom cais DNA. Thaum lub sij hawm gel-electrophoresis, agarose tsim ib qho nruab nrab gel matrix uas tuaj yeem yooj yim liquefied hauv qhov kub thiab txias tab sis yooj yim reverts hauv gel daim ntawv thaum txias.
Dab tsi yog qhov zoo sib xws ntawm Agar thiab Agarose?
- Ob Agar thiab agarose yog cov polysaccharides siab molecular-luv tau muab rho tawm los ntawm cov phab ntsa ntawm tes ntawm qee yam marine liab algae.
- Lawv muaj txiaj ntsig zoo li cov neeg ua haujlwm khov kho hauv kev tshawb fawb microbiological thiab molecular biological.
- Kuj, ob qho tib si khov ntawm qhov txias txias tab sis, yaj ntawm qhov kub siab.
- Agar thiab agarose yog qhov tsim nyog rau kev tshawb fawb microbiological thiab rau kev coj tus kab mob.
- Dhau li ntawd, ob leeg zoo li gelatin.
Qhov txawv ntawm Agar thiab Agarose yog dab tsi?
Ob Agar thiab agarose yog polysaccharides. Agar tuaj ncaj qha los ntawm cov algae liab thaum peb tau txais agarose los ntawm agar los ntawm kev ua kom huv ntxiv ntawm agar. Qhov tseem ceeb, agarose yog qhov tseem ceeb ntawm agar. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm agar thiab agarose. Tsis tas li ntawd, agarose yog ib qho linear polysaccharide thaum agar muaj li ntawm agarose thiab agaropectin. Agar ntau lawm yog siv sij hawm tsawg dua thiab cov txheej txheem tsis yooj yim dua li cov khoom lag luam agarose. Tsis tas li ntawd, kev siv cov agar feem ntau yog nyob rau hauv kev lag luam zaub mov ua cov khoom xyaw rau jellies, ice cream, mizuyokan, thiab gulaman thaum lub ntsiab siv ntawm agarose yog nyob rau hauv gel electrophoresis. Yog li, qhov no yog lwm qhov sib txawv ntawm agar thiab agarose.
Daim ntawv sib piv hauv qab no ntawm qhov sib txawv ntawm agar thiab agarose summarizes qhov sib txawv ntawm daim ntawv tabular.
Summary – Agar vs Agarose
Agar thiab agarose yog ob polysaccharides muab tau los ntawm liab algae. Structurely, agar muaj ob yam khoom; uas yog, agarose thiab agaropectin thaum agarose muaj agarobiose uas yog disaccharide. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm agar thiab agarose. Tsis tas li ntawd, agar yog pheej yig dua agarose txij li thaum peb tau txais agarose los ntawm kev ua kom huv ntxiv ntawm agar. Kev siv lub ntsiab ntawm agar yog ua tus neeg sawv cev hauv cov kab lis kev cai microbiological thaum siv lub ntsiab ntawm agarose yog nyob rau hauv gel electrophoresis.
