Qhov sib txawv tseem ceeb ntawm electrophoresis thiab chromatography yog cov khoom hluav taws xob ntawm cov tshuaj lom neeg siv rau electrophoresis whereas qhov sib faib coefficient ntawm ib hom tshuaj yog siv rau chromatography.
Ob qho tib si electrophoresis thiab chromatography yog cov txheej txheem kuaj uas peb siv los soj ntsuam cov qauv. Txawm li cas los xij, chromatography muaj kev lag luam siv ntau dua thiab muaj txiaj ntsig zoo rau cov ntim loj thaum electrophoresis yog ib qho txheej txheem tshawb nrhiav uas peb siv rau qib microscopic.
YElectrophoresis yog dab tsi?
Electrophoresis yog cov txheej txheem kuaj uas peb siv los tshuaj xyuas tus qauv siv cov khoom hluav taws xob ntawm cov tshuaj muaj nyob hauv cov qauv ntawd. Nyob ntawd, peb tuaj yeem soj ntsuam qhov kev txav ntawm cov khoom tawg hauv cov qauv. Li no, peb tuaj yeem txiav txim siab qhov kev txav ntawm cov tshuaj lom neeg txheeb ze rau cov kua dej uas nws muaj. Txawm li cas los xij, peb yuav tsum tsim qee yam tshwj xeeb. Piv txwv li, peb yuav tsum muab cov kua dej los ntawm qhov chaw hluav taws xob tsis sib xws. Txoj kev xav tom qab cov txheej txheem no yog qhov sib txawv ntawm qhov sib txawv ntawm qhov nruab nrab ntawm kev txav mus los ntawm qhov sib txawv ntawm kev tsiv teb tsaws chaw nyob rau hauv lub xub ntiag ntawm hluav taws xob teb.
Daim duab 01: Txoj Kev xav qab Electrophoresis
Ib lub ntsiab lus rau electrophoresis yog "electrokinetic phenomena". Tsis tas li ntawd, nyob ntawm seb hom ion tam sim no hauv cov qauv, peb tuaj yeem faib cov electrophoresis rau hauv ob pawg. Namely, lawv yog cataphoresis thiab anaphoresis. Cataphoresis yog rau cations (positively charged ions) thaum anaphoresis yog rau anions (tsis zoo them ions). Daim ntawv thov tseem ceeb tshaj plaws ntawm electrophoresis yog nyob rau hauv extraction ntawm DNA tawg raws li nws loj.
Chromatography yog dab tsi?
Chromatography yog ib qho kev tshuaj ntsuam xyuas uas peb siv los txheeb xyuas cov qauv uas siv qhov sib faib coefficients ntawm cov tshuaj muaj nyob hauv cov qauv. Txoj kev no yog qhov tseem ceeb heev hauv kev sib cais cov khoom hauv ib qho sib tov. Piv txwv li, chromatography yog cov txheej txheem tseem ceeb heev uas peb siv hauv kev ua cov ntshav tib neeg. Ntawm no, peb siv cov txheej txheem no los cais cov ntshav sib txawv, rau kev siv tshuaj kho mob.
Daim duab 02: Ib txheej nyias Chromatographic ntawv
Hauv cov txheej txheem no, peb siv ob theem ua theem mobile thiab nyob ruaj khov. Raws li, theem mobile yuav tsum muaj peb cov qauv, thiab cov theem nyob ruaj khov pab cais nws mus rau hauv cov khoom. Peb dhau theem mobile nrog cov qauv los ntawm cov theem nyob ruaj khov uas cov khoom hauv cov qauv txav ntawm qhov sib txawv. Qhov no ua rau cov khoom sib cais. Yog li, txoj kev xav tom qab cov txheej txheem yog qhov sib txawv ntawm qhov sib txawv ntawm cov khoom nruab nrab ntawm lub xov tooj ntawm tes thiab nyob ruaj khov.
Qhov txawv ntawm Electrophoresis thiab Chromatography yog dab tsi?
Electrophoresis yog cov txheej txheem kuaj uas peb siv los tshuaj xyuas tus qauv siv cov khoom siv hluav taws xob ntawm cov tshuaj lom neeg tam sim no nyob rau hauv cov qauv ntawd whereas chromatography yog cov txheej txheem tshuaj ntsuam xyuas uas peb siv los tshuaj xyuas cov qauv siv cov kev faib coefficients ntawm cov tshuaj muaj nyob hauv. tus qauv. Yog li, qhov no yog qhov sib txawv loj ntawm electrophoresis thiab chromatography. Ntxiv mus, qhov sib txawv ntawm electrophoresis thiab chromatography raws li kev siv yog tias peb tuaj yeem siv chromatography rau cov kua, khoom thiab gaseous compounds whereas peb feem ntau nqa tawm electrophoresis ntawm cov kua thiab cov khoom sib txuas nkaus xwb.
Summary – Electrophoresis vs Chromatography
Electrophoresis thiab chromatography cov txheej txheem tau hloov pauv txoj hauv kev tshawb nrhiav uas peb ua hauv chav kuaj. Yog li ntawd, cov tswv yim no muab qhov tseem ceeb hauv kev kawm txog DNA qauv thiab tshawb pom cov kab mob cuam tshuam nrog nws. Electrophoresis tau ua DNA thiab cov noob caj noob ces ua haujlwm yooj yim thaum chromatography tau muab rau tib neeg txoj kev ywj pheej los siv tag nrho cov ntshav kom zoo. Qhov sib txawv tseem ceeb ntawm electrophoresis thiab chromatography yog tias cov khoom siv hluav taws xob ntawm cov tshuaj lom neeg siv rau electrophoresis whereas qhov sib faib coefficient ntawm ib hom tshuaj yog siv rau chromatography.
