Qhov sib txawv tseem ceeb ntawm kev sib pauv ion thiab qhov loj me tsis suav nrog chromatography yog tias ion pauv chromatography yog siv los tshuaj xyuas cov tshuaj ionic raws li tus nqi whereas loj cais chromatography yog siv los tshuaj xyuas cov molecules loj raws li qhov loj me.
Chromatography yog cov txheej txheem kev tshuaj xyuas uas tuaj yeem siv los txheeb xyuas cov khoom sib txawv. Cov txheej txheem chromatographic muaj npe raws li qhov ntsuas siv los txheeb xyuas cov tshuaj - piv txwv li. Qhov loj cais chromatography, ion pauv chromatography, thiab lwm yam.
Ion Exchange Chromatography yog dab tsi?
Ion pauv chromatography yog cov txheej txheem tshuaj xyuas uas peb tuaj yeem txheeb xyuas cov tshuaj ionic. Nws yog feem ntau pab tau los txheeb xyuas cov anions thiab cations inorganic (piv txwv li chloride thiab nitrate anions thiab potassium, sodium cations). Txawm hais tias nws tsis yog ib txoj hauv kev zoo heev, peb tuaj yeem txheeb xyuas cov organic ions siv nws. Tsis tas li ntawd, peb tuaj yeem siv cov txheej txheem no rau kev ua kom huv ntawm cov protein vim tias cov proteins raug them cov roj molecules ntawm qee qhov pH qhov tseem ceeb. Ntawm no, peb siv lub sijhawm ruaj khov uas cov khoom them tuaj yeem txuas tau. Piv txwv li, peb tuaj yeem siv cov resin polystyrene-divinylbenzene copolymer ua cov khoom txhawb nqa.
Daim duab 01: Kev xav tom qab Ion Exchange Chromatography
Txhawm rau piav qhia qhov no ntxiv, theem nyob ruaj khov tau kho ions xws li sulfate anions lossis quaternary amine cations. Txhua yam ntawm cov no yuav tsum koom nrog lub txee ion (ib qho ion nrog cov nqi sib txawv) yog tias peb yuav tsum tswj qhov nruab nrab ntawm qhov system no. Yog hais tias lub txee ion yog ib qho cation, ces peb muab lub npe hu ua cation exchange resin. Tab sis, yog hais tias tus counterion yog ib qho anion, lub kaw lus yog anion exchange resin. Tsis tas li ntawd, muaj tsib kauj ruam tseem ceeb hauv ion pauv chromatography:
- pib theem
- Adsorption ntawm lub hom phiaj
- pib ntawm elution
- End of elution
- Regeneration
Dab tsi yog Qhov Cuam Tshuam Chromatography?
Size cais chromatography lossis SEC yog cov txheej txheem tshuaj ntsuam xyuas uas cov molecules hauv cov sib tov tuaj yeem sib cais los ntawm lawv qhov loj thiab qhov hnyav. Feem ntau, nws ua haujlwm nrog cov molecules loj hu ua macromolecules. Feem ntau, cov tshuaj aqueous yog siv rau kev tshuaj xyuas. Tom qab ntawd nws yog hu ua gel filtration chromatography. Thaum cov kuab tshuaj organic, nws hu ua gel permeation chromatography.
Gel filtration chromatography yog ib daim ntawv ntawm qhov loj me tsis suav nrog chromatography uas peb siv cov tshuaj aqueous raws li lub sijhawm txawb. Yog li ntawd, lub sij hawm mobile peb feem ntau siv nyob rau hauv cov txheej txheem no yog ib tug aqueous tsis. Tsis tas li ntawd, peb siv lub chromatographic kem rau qhov kev sib cais no, thiab peb yuav tsum tau ntim cov kem nrog cov hlaws dai. Feem ntau, Sephadex thiab agarose muaj txiaj ntsig zoo li cov khoom ntxeem tau. Yog li, cov khoom siv no yog lub sijhawm nyob ruaj khov ntawm peb qhov kev sim. Ntxiv mus, peb tuaj yeem siv qhov pore loj ntawm cov hlaws no los txiav txim qhov loj ntawm macromolecules uas peb tau sib cais. Txawm li cas los xij, nws tsuas yog kev kwv yees xwb.
Daim duab 02: Ib qho cuab yeej rau qhov loj me me tsis suav nrog Chromatography
Gel permeation chromatography yog ib daim ntawv ntawm qhov loj me ntawm chromatography uas peb siv cov organic hnyav raws li theem mobile. Yog li, peb tuaj yeem siv cov kev daws teeb meem xws li hexane thiab toluene rau lub hom phiaj no. Cov theem nyob ruaj ruaj yog cov khoom ntxeem tau, zoo ib yam li hauv gel filtration chromatography. Cov txheej txheem no feem ntau siv rau polymers thiab lwm yam khoom siv organic-soluble. Cov txheej txheem ntawm kev ua ntawm cov txheej txheem zoo ib yam li cov gel filtration chromatography.
Dab tsi yog qhov txawv ntawm Ion Txauv thiab Qhov Cuam Tshuam Tsis Txaus Siab Chromatography?
Chromatography yog cov txheej txheem kev tshuaj xyuas uas tuaj yeem siv los txheeb xyuas cov khoom sib txawv. Qhov sib txawv tseem ceeb ntawm kev sib pauv ion thiab qhov loj me tsis suav nrog chromatography yog tias ion pauv chromatography yog siv los tshuaj xyuas cov tshuaj ionic raws li tus nqi thaum qhov loj cais chromatography yog siv los tshuaj xyuas cov molecules loj raws li qhov loj me.
Hauv qab infographic muab cov ntaub ntawv ntxiv txog qhov sib txawv ntawm ion pauv thiab qhov loj me ntawm chromatography.
Summary – Ion Txauv vs Loj Tsis suav nrog Chromatography
Chromatography yog cov txheej txheem kev tshuaj xyuas uas tuaj yeem siv los txheeb xyuas cov khoom sib txawv. Qhov sib txawv tseem ceeb ntawm kev sib pauv ion thiab qhov loj me tsis suav nrog chromatography yog tias ion pauv chromatography yog siv los tshuaj xyuas cov tshuaj ionic raws li tus nqi thaum qhov loj cais chromatography yog siv los tshuaj xyuas cov molecules loj raws li qhov loj me.
