Qhov sib txawv tseem ceeb ntawm ion khub thiab ion pauv chromatography yog tias, hauv ion khub chromatography, ions hauv cov qauv tuaj yeem "paired" thiab sib cais raws li tus khub ion whereas, hauv ion pauv chromatography, ions hauv cov qauv tuaj yeem muab cais ua cations thiab anions nyias.
Chromatography yog cov txheej txheem tseem ceeb uas ua rau kev sib cais ntawm cov khoom sib txawv hauv kev sib tov. Ion khub thiab ion pauv chromatography yog cov txheej txheem kev tshuaj ntsuam peb tuaj yeem siv los cais cov ions thiab cov polar molecules hauv kev sib xyaw, raws li cov nqi hluav taws xob uas lawv nqa nrog lawv.
Ion Pair Chromatography yog dab tsi?
Ion khub chromatography yog cov txheej txheem tshuaj xyuas qhov twg ions hauv cov qauv raug ua khub thiab sib cais raws li cov khub ion. Ion pairing hais txog qhov nruab nrab; Thaum cations khub nrog anions, lawv cov nqi hluav taws xob neutralize. Ntawm no, cov txheej txheem kev sib cais no yog ua tiav hauv kab rov qab-theem. Hauv cov txheej txheem no, peb yuav tsum siv ion-pairing agents txhawm rau tsim cov ion khub thiab cais cov ions hauv cov qauv. Feem ntau, cov ion-pairing agents yog cov sib txuas uas muaj cov saw hlau hydrocarbon. Cov ion-pairing cov neeg ua haujlwm no yuav tsum muaj cov hluav taws xob sib txawv rau cov ions hauv cov qauv; txwv tsis pub, ions yuav tsis ua khub (ions nrog tib tus nqi tsis ua khub, vim lawv tawm tsam ib leeg). Tsis tas li ntawd, cov ion-pairing cov neeg ua haujlwm tuaj yeem ua rau kom muaj dej thiab tuav tau zoo.
Tsis tas li ntawd, siv ion-pair tus neeg sawv cev raws li theem ntawm lub xov tooj ntawm tes tso cai rau peb cais cov ionic thiab cov polar ntau tau yooj yim. Piv txwv li, yog hais tias peb ntxiv ib tug reagent uas muaj ib tug hydrophobic functional pab pawg neeg, cov theem nyob ruaj ruaj yuav khaws tau no hydrophobic functional pawg; Yog li, khub ions kuj tseem khaws cia nyob rau theem nyob ruaj khov nrog rau cov pab pawg ua haujlwm hydrophobic ntxiv. 1-pentylsodiumsulfonate thiab 1-hexylsodiumsulfonate yog ib qho tseem ceeb raws li cov anionic counter ions rau cations tam sim no nyob rau hauv cov qauv thiab 1-pentanesulfonate yog ib qho tseem ceeb raws li ib tug cationic counter ion rau anions.
Qhov zoo ntawm Ion Pair Chromatography
Muaj ntau qhov zoo ntawm ion khub chromatography thaum piv rau ion pauv chromatography;
- Yooj yim los npaj cov kev daws teeb meem tsis xav tau
- tuaj yeem xaiv ntau yam ntawm cov saw hlau ntev hauv ion-pairing agents
- Kev tshwm sim yog rov tsim tawm heev
- Yuav tau txais cov duab zoo dua qub
- Txo lub sij hawm sib cais
Ion Exchange Chromatography yog dab tsi?
Ion pauv chromatography yog ib hom kua chromatography uas peb tuaj yeem txheeb xyuas cov tshuaj ionic. Peb feem ntau siv nws los tshuaj xyuas cov anions thiab cations inorganic (piv txwv li chloride thiab nitrate anions thiab potassium, sodium cations). Txawm hais tias nws tsis tshua muaj, peb tuaj yeem txheeb xyuas cov organic ions thiab. Tsis tas li ntawd, peb tuaj yeem siv cov txheej txheem no rau kev ua kom huv ntawm cov protein vim tias cov proteins raug them cov roj molecules ntawm qee qhov pH qhov tseem ceeb. Ntawm no, peb siv lub sijhawm ruaj khov uas cov khoom them tuaj yeem txuas tau. Piv txwv li, peb tuaj yeem siv cov resin polystyrene-divinylbenzene copolymer ua cov khoom txhawb nqa.
Txhawm rau piav qhia qhov no ntxiv, theem nyob ruaj khov tau kho ions xws li sulfate anions lossis quaternary amine cations. Txhua yam ntawm cov no yuav tsum koom nrog lub txee ion (ib qho ion nrog cov nqi sib txawv) yog tias peb yuav tsum tswj qhov nruab nrab ntawm qhov system no. Yog hais tias lub txee ion yog ib qho cation, ces peb muab lub npe hu ua cation exchange resin. Tab sis, yog hais tias tus txee ion yog anion, lub kaw lus yog anion pauv resin. Tsis tas li ntawd, muaj tsib kauj ruam tseem ceeb hauv ion pauv chromatography:
- pib theem
- Adsorption ntawm lub hom phiaj
- pib ntawm elution
- End of elution
- Regeneration
Qhov txawv ntawm Ion Pair thiab Ion Txauv Chromatography yog dab tsi?
Ion khub thiab ion-exchange chromatographies yog cov txheej txheem analytical peb tuaj yeem siv los cais cov ions thiab cov polar molecules hauv kev sib tov. Qhov sib txawv tseem ceeb ntawm ion khub thiab ion txauv chromatography yog tias, hauv ion-pair chromatography, peb tuaj yeem ua ions hauv cov qauv "paired" thiab cais nws ua tus khub ion, thaum nyob rau hauv ion pauv chromatography, peb tuaj yeem cais cov ions hauv. tus qauv ua cations thiab anions nyias.
Hauv qab infographic qhia ntau qhov kev sib piv ntsig txog qhov sib txawv ntawm ion khub thiab ion pauv chromatography.
Summary – Ion Pair vs Ion Exchange Chromatography
Ion khub thiab ion pauv chromatography yog cov txheej txheem tshuaj ntsuam peb tuaj yeem siv los cais cov ions thiab cov polar molecules hauv kev sib xyaw. Qhov sib txawv tseem ceeb ntawm ion khub thiab ion txauv chromatography yog tias nyob rau hauv ion-pair chromatography, ions hauv cov qauv tuaj yeem "paired" thiab sib cais raws li tus khub ion whereas, hauv ion pauv chromatography, ions hauv cov qauv tuaj yeem sib cais ua cations. thiab anions cais.
