Qhov sib txawv tseem ceeb ntawm gel pom thiab gel permeation chromatography yog tias lub sijhawm txawb ntawm gel pom chromatography yog cov tshuaj aqueous whereas lub sijhawm txawb ntawm gel permeation chromatography yog cov kuab tshuaj organic.
Ob lub gel pom thiab gel permeation chromatography tuaj nyob rau hauv qeb ntawm qhov loj me tsis suav nrog chromatography uas peb tuaj yeem cais cov molecules hauv cov tshuaj nyob ntawm lawv qhov loj me. Ntawd yog, qee zaum, peb tuaj yeem cais cov molecules raws li lawv qhov hnyav molecular. Feem ntau, peb siv cov tswv yim no los cais cov molecules nyuaj xws li cov proteins thiab hauv kev lag luam nplai, cais cov ntaub ntawv polymer. Qhov sib txawv tseem ceeb ntawm gel pom thiab gel permeation chromatography yog nyob rau hauv lub xov tooj ntawm tes peb siv nyob rau hauv txhua txoj kev.
Gel Filtration Chromatography yog dab tsi?
Gel filtration chromatography yog ib daim ntawv ntawm qhov loj me tsis suav nrog chromatography uas peb siv cov tshuaj aqueous raws li lub sijhawm txawb. Yog li ntawd, feem ntau ntawm lub sij hawm, lub mobile theem uas peb siv nyob rau hauv cov txheej txheem no yog ib tug aqueous tsis. Tsis tas li ntawd, peb siv lub chromatographic kem rau qhov kev sib cais no, thiab peb yuav tsum tau ntim cov kem nrog cov hlaws dai. Feem ntau, Sephadex thiab agarose muaj txiaj ntsig zoo li cov khoom ntxeem tau. Yog li, cov khoom siv no yog lub sijhawm nyob ruaj khov ntawm peb qhov kev sim. Ntxiv mus, peb tuaj yeem siv qhov pore loj ntawm cov hlaws no los txiav txim qhov loj ntawm macromolecules uas peb tau sib cais. Txawm li cas los xij, nws tsuas yog kev kwv yees xwb.
Daim duab 01: Apparatus rau Gel Filtration Chromatography
Daim ntawv thov tseem ceeb ntawm cov txheej txheem no yog cais cov proteins thiab lwm yam khoom siv dej-soluble raws li qhov loj me. Cov txheej txheem ua haujlwm los ntawm kev ntxiab cov khoom me me hauv qhov pores ntawm lub sijhawm nyob ruaj khov (cov hlaws dai) thaum cov molecules loj elute los ntawm cov gel. Yog li ntawd, thawj feem muaj cov molecules loj. Tom qab ntawd peb tuaj yeem siv cov kuab tshuaj sib txawv uas tuaj yeem tshem tawm cov me me hauv cov pores. Tom qab ntawd peb feem thib ob muaj cov molecules me me.
YGel Permeation Chromatography yog dab tsi?
Gel permeation chromatography yog ib daim ntawv ntawm qhov loj me ntawm chromatography uas peb siv cov organic hnyav raws li theem mobile. Yog li, peb tuaj yeem siv cov kev daws teeb meem xws li hexane thiab toluene rau lub hom phiaj no.
Daim duab 02: Gel Permeation Chromatographic Instrument
theem nyob ruaj khov yog cov khoom ntxeem tau zoo ib yam li hauv gel pom chromatography. Cov txheej txheem no feem ntau siv tau rau cov polymers thiab lwm yam khoom siv organic-soluble. Cov txheej txheem ntawm kev ua ntawm cov txheej txheem zoo ib yam li cov gel filtration chromatography.
Qhov txawv ntawm Gel Lim thiab Gel Permeation Chromatography yog dab tsi?
Gel filtration thiab gel permeation chromatography yog ob daim ntawv ntawm qhov loj me tsis suav nrog chromatography uas koom nrog kev sib cais ntawm cov molecules hauv cov qauv raws li qhov loj ntawm cov molecules. Qhov sib txawv ntawm gel filtration thiab gel permeation chromatography yog nyob rau hauv lub xov tooj ntawm tes lawv siv thiab li no, daim ntawv thov ntawm cov txheej txheem. Yog li ntawd, qhov sib txawv tseem ceeb ntawm gel pom thiab gel permeation chromatography yog tias lub sijhawm txawb ntawm gel filtration chromatography yog cov tshuaj aqueous thaum lub sijhawm txawb ntawm gel permeation chromatography yog cov organic hnyav.
Cov hauv qab no qhia txog qhov sib txawv ntawm gel pom thiab gel permeation chromatography hauv daim ntawv.
Summary – Gel Lim vs Gel Permeation Chromatography
Size cais chromatography yog nyob rau hauv ob hom loj li gel pom thiab gel permeation chromatography. Qhov sib txawv tseem ceeb ntawm gel pom thiab gel permeation chromatography yog tias lub xov tooj ntawm tes ntawm gel pom chromatography yog cov tshuaj aqueous thaum lub sijhawm txawb ntawm gel permeation chromatography yog cov organic hnyav.
