Qhov sib txawv tseem ceeb ntawm tag nrho cov genome sequencing thiab exome sequencing yog tias tag nrho cov genome sequencing sequences tag nrho cov genome ntawm ib yam kabmob thaum lub exome sequences tsuas yog exome los yog protein-coding noob ntawm ib tug kab mob.
Sequencing yog txheej txheem uas txiav txim siab qhov kev txiav txim ntawm nucleotides hauv ib qho DNA molecule. Nws tsuas yog qhia txog qhov tseeb ntawm plaub lub hauv paus; adenine (A), guanine (G), cytosine (C) thiab thymine (T) hauv DNA tawg. Muaj ntau ntau txoj hauv kev xws li Sanger sequencing, tiam tom ntej sequencing, thiab lwm yam. Tsis tas li ntawd, txawm tias tag nrho cov genome ntawm cov kab mob (tag nrho cov genome sequencing) los yog tsuas yog cov protein-coding genes hauv genome (exome sequencing) tuaj yeem ua tiav.
Txhua Genome Sequencing yog dab tsi?
Tus tib neeg genome muaj yuav luag peb txhiab tus lej ntawm DNA. Tag nrho genome sequencing yog ib txoj hauv kev uas ua kom tiav cov genome ntawm ib yam kabmob. Yog li ntawd, nyob rau hauv tag nrho genome sequencing, qhov kev txiav txim siab yog nyob rau hauv tag nrho cov sau ntawm DNA molecules xws li exomes, introns, intergenic qhov chaw, thiab lwm yam. Tsis tas li ntawd, tag nrho cov genome sequencing sequences tsis tsuas yog lub genome uas nyob hauv lub nucleus tab sis kuj mitochondrial DNA thiab..
Daim duab 01: Tag Nrho Genome Sequencing
Nyob rau hauv kev npaj rau qhov sequencing no, thawj kauj ruam yog rho tawm tag nrho DNA. Tom qab ntawd, cov DNA rho tawm tau dhau los ua cov txheej txheem sib txuas uas siv cov txheej txheem tsim nyog. Los ntawm kev txheeb xyuas qhov tshwm sim, siv cov cuab yeej bioinformatics, peb tuaj yeem pom cov kab mob tsawg, mob qog noj ntshav, kab mob sib kis, thiab lwm yam.
Exome Sequencing yog dab tsi?
Exome yog ib feem ntawm cov genome uas muaj txog 1.5% ntawm cov genome, thiab nws yog cov sau ntawm exons. Exons yog cov sequences uas sau rau hauv mRNA thiab tom qab ntawd mus rau cov khoom protein kawg. Li no, exome sequencing sequences tag nrho cov protein-coding sequences nyob rau hauv lub genome. Exome sequencing qhia txog cov kab mob caj ces vim feem ntau ntawm cov kab mob caj ces tshwm sim vim kev hloov pauv ntawm cov protein-coding noob. Yog li, cov txheej txheem kuaj mob ntawm caj ces feem ntau siv exome sequencing raws li ib txoj hauv kev los kuaj tus kab mob. Tsis tas li ntawd, thaum muab piv nrog tag nrho cov genome sequencing daim ntawv thov rau kev ntsuam xyuas caj ces, qhov exome sequencing yuav siv sij hawm tsawg dua los ua hauj lwm thiab tsis kim.
Daim duab 02: Exome Sequencing
Nyob rau hauv exome sequencing, ua ntej, nws yog ib qho tsim nyog los xaiv cov protein coding sequences los ntawm tag nrho DNA. Nco ntsoov tias tib neeg muaj txog 180000 protein-coding sequences. Tom qab ntawd, tus exome yog sequenced siv ib tug high throughput sequencing txoj kev.
Dab tsi yog qhov zoo sib xws ntawm Tag Nrho Genome Sequencing thiab Exome Sequencing?
- Tag Nrho Genome Sequencing thiab Exome Sequencing yog ob daim ntawv thov ntawm sequencing.
- Ob txoj hauv kev yog qhov tseem ceeb hauv kev txiav txim siab qhov tseeb ntawm nucleotide ib ntus hauv ob txoj hauv kev.
- Tsis tas li, bioinformatics pab hauv ob txoj kev los tshuaj xyuas cov ntaub ntawv.
Dab tsi yog qhov txawv ntawm Tag Nrho Genome Sequencing thiab Exome Sequencing?
Tag nrho genome sequencing sequences tag nrho DNA ntawm genome ntawm ib yam kab mob, thaum lub exome sequences tsuas yog cov protein coding sequences los ntawm genome. Yog li, qhov no yog qhov sib txawv ntawm tag nrho genome sequencing thiab exome sequencing. Tsis tas li ntawd, txawm hais tias ob txoj hauv kev tau siv dav los txiav txim siab qhov tseeb ntawm nucleotide ib ntus, tag nrho cov genome sequencing yog kim thiab siv sij hawm ntau dua li exome sequencing.
Cov hauv qab no infographic tabulates qhov sib txawv ntawm tag nrho genome sequencing thiab exome sequencing.
Summary – Whole Genome Sequencing vs Exome Sequencing
Sequencing qhia meej meej ntawm cov hauv paus hauv DNA tawg thiab nws tuaj yeem yog tag nrho genome sequencing lossis exome sequencing. Ntawm no, tag nrho cov genome sequencing sequences tag nrho genome ntawm ib tug kab mob thaum lub exome sequences tsuas yog cov protein-coding sequences nyob rau hauv lub genome. Xav txog lawv cov ntawv thov, tag nrho cov genome sequencing yog pab tau rau hauv kev tshuaj ntsuam genetic aberrations xws li ib tug nucleotide variations, deletions, insertions, thiab lwm yam nyob rau hauv tag nrho cov genome thaum lub exome sequencing yog pab tau nyob rau hauv kev soj ntsuam cov kab mob ntawm caj ces. Yog li, qhov no yog qhov sib txawv ntawm tag nrho genome sequencing thiab exome sequencing.
