Qhov Tseem Ceeb - Phusion vs Taq Polymerase
DNA polymerases tau siv dav hauv cov enzymes hauv cov txheej txheem biology molecular thiab tseem muaj nyob rau hauv txhua yam kab mob uas tau txais DNA replication. Lawv yog cov tseem ceeb polymerizing enzymes uas koom nrog thaum lub sij hawm replication. DNA polymerase muaj peev xwm ntxiv nucleotides rau qhov dawb 3' kawg ntawm DNA strand yog li ua rau txuas ntxiv ntawm txoj hlua tshiab. Tam sim no, vim yog kev txhim kho ntawm molecular biology hauv kev kuaj kab mob thiab kev siv hauv kev lag luam, nws yog ib qho tseem ceeb los tsim cov polymerases uas muaj ntau yam txiaj ntsig. Qhov no ua rau kom qhov tseeb ntawm txoj kev thiab ua rau nws cov txheej txheem nrawm dua. Phusion thiab Taq polymerase yog ob lub lag luam ua lag luam thermostable polymerase enzymes uas siv rau hauv cov ntawv thov tshwj xeeb molecular. Phusion yog DNA polymerase cais los ntawm Pyrococcus furiosus thiab feem ntau yog siv rau hauv kev sim cloning kom muaj kev ncaj ncees. Taq DNA Polymerase yog tus qauv DNA polymerase siv hauv Polymerase Chain Reaction (PCR), thiab nws raug cais tawm ntawm cov kab mob thermostable; Thermus aquaticus. Qhov sib txawv tseem ceeb ntawm ob lub enzymes yog cov kab mob microorganisms. Phusion yog cais tawm ntawm extremophile, Pyrococcus furiosus whereas, Taq polymerase yog cais los ntawm cov thermophile, Thermus aquaticus.
Phusion yog dab tsi?
Phusion DNA polymerase yog ib qho tshiab polymerase uas yog tsim los ntawm kev cais cov enzyme los ntawm Pyrococcus furiosus, uas yog extremophilic Archaea. Cov microbes nyob hauv qhov kub thiab txias heev, yog li ua rau cov polymerase ua kom sov-ruaj khov polymerase. Phusion polymerase yog siv los ua kom muaj kev ncaj ncees tshaj li cov qauv thermostable polymerase; Cov tshuaj polymerase. Phusion polymerase muaj peev xwm nthuav dav cov qauv ntev txog 7.5kb ntawm genomic DNA. Qhov zoo tshaj plaws polymerization muaj peev xwm ntawm Phusion polymerase yog ntawm 72 0C. Phusion kuj tseem siv rau hauv cov khoom siv cloning rau sequencing, qhia kev tsom xam thiab kev hloov pauv.
Phusion DNA polymerase muaj 3' - 5' exonuclease kev ua. Qhov no tso cai rau cov ntaub ntawv pov thawj ntawm cov khoom siv tshiab tshiab tom qab kev sib txuas. Yog li ntawd, cov nucleotide mismatches tau yooj yim kho. Yog li, nws muaj qhov yuam kev tsawg dua. Tag nrho qhov zoo ntawm Phusion polymerase yog;
- Kev Ncaj Ncees
- Kev nrawm nrawm thiab txo lub sijhawm txuas ntxiv
- Cov Kev Ua Phem Zoo thiab xav tau kev ua kom zoo tsawg tsawg
- High Yield
Daim duab 01: Phusion
Lub ntsiab tsis zoo ntawm Phusion polymerase yog tias nws yog inhibited nyob rau hauv lub xub ntiag ntawm deoxyuridine triphosphate (dUTP). Thaum dUTPs tau sau rau hauv cov tshuaj tiv thaiv sib xyaw, nws tuaj yeem cuam tshuam cov kev ua ntawm Phusion polymerase. Qhov no tiv thaiv los ntawm kev kho cov tshuaj tiv thaiv sib xyaw nrog dUTPase ua ntej ntxiv cov enzyme. Tam sim no, dUTP resistant variant ntawm Phusion polymerase lub npe hu ua Pfu Turbo yog siv es tsis txhob Phusion polymerase raws li kev kho rau qhov teeb meem no.
Taq Polymerase yog dab tsi?
Taq DNA polymerase invention tau hloov pauv lub teb ntawm molecular biology. Nws daws qhov teeb meem loj hauv DNA amplification. Taq DNA polymerase yog lub tshav kub ruaj khov polymerase enzyme muab rho tawm thiab cais tawm ntawm cov kab mob thermophilic, Thermus aquaticus. Kev tshawb pom ntawm cov enzymes no ua rau kev txhim kho ntawm PCR. Cov enzyme no tau tso cai rau kev siv cov tshuaj polymerase saw hauv DNA amplification es tsis txhob siv cov txheej txheem ua haujlwm cloning. PCR tam sim no siv rau hauv kev tshuaj ntsuam xyuas molecular, kev ua liaj ua teb thiab kev lag luam nrog ntau qhov kev hloov pauv tshiab ntxiv rau cov txheej txheem.
Daim duab 02: Taq Polymerase
Taq DNA polymerase ua haujlwm ntawm qhov ntsuas kub zoo ntawm 720C - 800C. Taq DNA polymerase yuav tsum muaj kev sib koom ua ke; magnesium rau nws txoj haujlwm. Taq polymerase tsis muaj 3' - 5' proofreading muaj peev xwm, yog li ntawd, qhov yuam kev ntawm Taq DNA polymerase yog siab nyob rau hauv kev sib piv rau ntau hom tshiab ntawm DNA polymerases xws li Phusion polymerase thiab lwm yam. Tab sis, qhov nrov ntawm Taq DNA polymerase tseem yog tus. tib yam thoob plaws ntiaj teb kev tshawb fawb vim qhov yooj yim thiab yooj yim ntawm cov enzyme.
Dab tsi yog qhov zoo sib xws ntawm Phusion thiab Taq polymerase?
- Ob leeg Phusion thiab Taq polymerase enzymes yog polymerizing enzymes uas muaj peev xwm ntxiv nucleotides rau 3 'dawb kawg ntawm DNA strand.
- Ob leeg Phusion thiab Taq polymerases xav tau cov txheej txheem primer los pib polymerization.
- Ob leeg Phusion thiab Taq polymerases yog qhov kub thiab txias.
- Ob leeg Phusion thiab Taq polymerases yog siv hauv PCR cov txheej txheem los nthuav dav DNA.
- Thaum ntxiv cov polymerase rau cov tshuaj tiv thaiv sib tov, ob qho tib si Phusion thiab Taq polymerases tau ntxiv kawg los xyuas kom meej qhov ua tau zoo ntawm cov enzyme.
- Ob leeg Phusion thiab Taq polymerases yog cov khoom lag luam ua ke rau kev sim tshuaj lom neeg biology.
- Ob leeg Phusion thiab Taq polymerases xav tau cofactor kom ua tiav nws txoj haujlwm.
Qhov txawv ntawm Phusion thiab Taq Polymerase yog dab tsi?
Phusion vs Taq Polymerase |
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| Phusion yog DNA polymerase cais tawm ntawm Pyrococcus furiosus thiab feem ntau yog siv hauv kev sim cloning kom muaj kev ncaj ncees. | Taq DNA Polymerase yog tus qauv DNA polymerase siv hauv Polymerase Chain Reaction (PCR), thiab nws raug cais tawm ntawm cov kab mob thermostable, Thermus aquaticus. |
| Lub Koom Haum | |
| Phusion yog muab rho tawm los ntawm extremophilic archaea - Pyrococcus furiosus. | Cov ntaub ntawv no nthuav tawm los ntawm lub sijhawm colonization mus rau lub sijhawm decolonization. |
| Kev Nyeem Ntawv Pov Thawj | |
| 3 ' - 5 ' pov thawj kev nyeem ntawv muaj peev xwm muaj nyob hauv Phusion. | 3 ' - 5 ' pov thawj kev nyeem ntawv tsis muaj nyob hauv Taq polymerase. |
| Kev ncaj ncees | |
| High fidelity is there with Phusion. | Taq polymerase qhia tau hais tias tsis muaj tseeb. |
| Amplification | |
| Phusion muaj peev xwm nthuav dav DNA tawg ntev. | Taq polymerase muaj peev xwm ua kom nthuav dav ntau cov DNA luv luv. |
| dUTP Tshuaj lom | |
| Phusion kev txiav txim yog inhibited los ntawm kev sib txuam sib txawv. | Taq polymerase tsis yog inhibited los ntawm dUPT. |
Summary – Phusion vs Taq polymerase
Phusion thiab Taq DNA polymerase yog ob lub tshav kub ruaj khov polymerases uas tau siv hauv PCR cov txheej txheem. Phusion yog ib qho polymerase cais tawm ntawm extremophile, Pyrococcus furiosus whereas, Taq yog cais los ntawm cov kab mob thermostable Thermus aquaticus. Kev tshawb pom ntawm taq DNA polymerase ua rau PCR tsim. Phusion muaj ntau qhov zoo dua Taq uas tau ua rau Phusion qhov kev xaiv zoo dua hauv kev tsim cov DNA fidelity siab. Txawm li cas los xij, Taq polymerase tseem siv los ua tus qauv polymerase enzyme hauv PCR. Qhov no yog qhov txawv ntawm Phusion thiab Taq polymerase.
