Qhov Tseem Ceeb Qhov txawv - Kab mob Transposases vs Retroviral Integrases
Cov khoom siv caj ces thauj tau hloov zuj zus nrog ob lub tswv yim tseem ceeb txav los ntawm ib cheeb tsam mus rau thaj tsam tom ntej ntawm sab hauv thiab nruab nrab ntawm cov genomes. Ib txoj hauv kev yog tshem tawm los ntawm RNA molecule ua ntej tsim DNA molecule thaum lwm txoj hauv kev cuam tshuam nrog DNA intermediates. Transposases thiab viral integrases yog cov piv txwv ntawm cov khoom siv noob caj noob ces. Cov kab mob transposases khi mus rau qhov kawg ntawm transposons thiab pab txhawb lub catalysis ntawm transposon txav mus rau lwm qhov ntawm genome los ntawm ntau yam txheej txheem. Retroviral integrases yog cov enzymes uas pab hauv kev sib koom ua ke ntawm cov khoom siv caj ces ntawm retrovirus xws li HIV rau hauv cov khoom siv caj ces (DNA) ntawm tus tswv tsev cell nws kis. Qhov no yog qhov sib txawv tseem ceeb ntawm cov kab mob transposases thiab retroviral integrases.
Dab tsi yog Kab Mob Transposases?
Transposase tuaj yeem txhais tau tias yog ib qho enzyme khi rau qhov kawg ntawm cov transposons uas pab txhawb cov catalysis ntawm transposon txav mus rau lwm qhov ntawm cov genome los ntawm ntau yam txheej txheem. Cov txheej txheem zoo li no suav nrog 'txiav thiab muab tshuaj txhuam' thiab 'txheej txheem hloov pauv'. Transposase yog thawj zaug qhia los ntawm cloning ntawm cov enzyme uas xav tau rau kev hloov pauv ntawm Tn3 transposon. Ob lub tswv yim tseem ceeb tau siv los ntawm kev hloov pauv cov noob caj noob ces rau kev hloov pauv ntawm cov genomes lossis los ntawm ib qhov chaw mus rau lwm qhov. Kev thauj mus los ntawm qhov nruab nrab ntawm RNA ua ntej kev sib txuas ntawm DNA luam yog ib lub tswv yim thaum lwm tus raug khi rau DNA intermediates ib leeg. Cov tshuaj tiv thaiv recombination uas koom nrog kev sib koom ua ke ntawm ob lub ntsiab lus tau tshwm sim vim yog cov ntsiab lus tshwj xeeb enzymes. Yog li, piv txwv li DNA cov ntsiab lus, cov enzymes no hu ua transposases thaum nyob rau hauv ib qho piv txwv ntawm RNA ntsiab, lawv hu ua integrases.
Thaum muab piv qhov sib txawv ntawm ob qho kev hloov pauv cov tswv yim, cov txheej txheem ntawm kev ntxig tau tshwm sim zoo ib yam tshuaj lom neeg. Tab sis, cov pov thawj tsis ntev los no qhia tias qee qhov zoo sib xws hauv kev sib koom ua ke tau pom nyob rau hauv thaj tsam ntawm cov amino acid sequences uas tsim ib qho chaw nquag; lub DDE motif. Tsib tsev neeg ntawm transposase tau raug cais tawm tam sim no tab sis, cov tsev neeg tseem tsis tau nce ntxiv nrog cov cim tshiab transposase. Cov tsev neeg suav nrog DDE transposase, Tyrosine (Y) transposase, Serine (S) transposase, Rolling circle transposase, Reverse transcriptases/endonucleases (RT/En) thiab lwm yam. Cov tsev neeg no siv cov cuab yeej tshwj xeeb catalytic rau kev tawg thiab rov ua dua ntawm DNA. Lub DDE transposase koom nrog hauv kev txiav thiab muab tshuaj txhuam ntawm qhov qub transposon thiab nqa peb pawg ntawm kev txuag cov amino acids uas yog; aspartate (D), aspartate (D) thiab glutamate (E). Cov tyrosine transposases kuj koom nrog hauv kev txiav thiab muab tshuaj txhuam los ntawm kev siv tyrosine residue, uas yog qhov chaw tshwj xeeb.
Daim duab 01: Cov kab mob kis kab mob
Cov serine transposases koom nrog qhov nruab nrab ntawm DNA ncig thiab ua tiav cov txheej txheem txiav thiab muab tshuaj raws li cov tsev neeg saum toj no. Lub voj voog rolling transposase koom nrog hauv daim ntawv theej- hauv cov txheej txheem uas ib txoj hlua txuas ncaj qha rau hauv lub hom phiaj ntawm qhov chaw los ntawm DNA replication. Qhov no ua kom cov template strand thiab the copy strand muaj ib strand uas yog tshiab synthesized. Qhov thim rov qab transcriptases / endonucleases transposase muaj ntau yam kev hloov pauv.
Retroviral Integrases yog dab tsi?
Nyob rau hauv cov ntsiab lus ntawm Retroviral Integrase, nws yog suav hais tias yog ib tug retroviral enzyme uas pab nyob rau hauv kev koom ua ke ntawm cov noob caj noob ces ntawm retrovirus xws li HIV mus rau hauv cov genetic khoom (DNA) ntawm lub cell uas kis tau tus kab mob. Cov retroviral integrases feem ntau tau tsis meej pem nrog phage integrases. Piv txwv rau phage integrases yog λ phage integrase. Tab sis cov no yog cov enzymes sib txawv kiag li thiab yuav tsum tsis txhob totaub nrog. Nyob rau hauv kev hwm rau kev tsim ntawm retroviral pre-integration complex, retroviral integrase plays lub luag haujlwm tseem ceeb. Retroviral integrase proteins feem ntau muaj peb (03) canonical domains. Cov npe no tau txuas nrog cov txuas yooj yim.
Peb lub npe suav nrog N davhlau ya nyob twg zinc-binding domain uas peb lub pob helical txuas nrog thiab ruaj khov los ntawm kev sib koom tes nrog kev koom tes ntawm Zn 2+ cation, ib qho RNase H quav catalytic core domain thiab C terminal DNA binding domain, uas yog SH3 quav. Los ntawm kev tshawb nrhiav thiab los ntawm cov ntaub ntawv biochemical thiab cov qauv, nws qhia tias kev sib koom ua ke ntawm retroviral muaj peev xwm ua haujlwm raws li ib qho dimer ntawm dimmers (tetramer). Nyob rau hauv cov ntsiab lus ntawm multimerisation thiab kab mob DNA binding, tag nrho peb yam ntawm cov retroviral integrase protein. Lub luag haujlwm tseem ceeb ntawm retroviral integrase yog los ntxig nws cov khoom siv caj ces los tuav DNA. Cov kauj ruam no yog kauj ruam tseem ceeb tshaj plaws hauv kev kis tus kab mob HIV. Thaum ua tiav kev sib koom ua ke, nws yuav nyob rau hauv chromosomal DNA ntawm lub cell rau tag nrho nws lub neej.
Daim duab 02: Kev sib xyaw ua ke ntawm Retroviral
Yog li ntawd, ib zaug kev koom ua ke yuav tsis rov qab los rau lub xovtooj. Cov retroviral integrases no koom nrog hauv kev ua kom muaj ob qho kev cuam tshuam loj xws li 3 'kev ua tiav thiab covalent ligation. Thaum lub sij hawm 3' kawg ua, 2-3 nucleotides los ntawm ob qho tib si 3' kawg ntawm tus kab mob DNA raug tshem tawm nrog lub hom phiaj ntawm kev nthuav tawm CA dinucleotides ntawm 3' kawg ntawm cov kab mob DNA, thiab thaum lub sij hawm covalent ligation, cov txheej txheem 3' kawg ntawm DNA kab mob yog ligated covalently rau hauv tus tswv chromosomal DNA.
Dab tsi yog qhov zoo sib xws ntawm Bacterial Transposases thiab Retroviral Integrases?
Ob qho tib si Bacterial Transposases thiab Retroviral Integrases muaj cov amino acid zoo sib xws
Dab tsi yog qhov txawv ntawm Bacterial Transposases thiab Retroviral Integrases?
Cov kab mob kis kab mob vs Retroviral Integrases |
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| Cov kab mob Transposase yog ib qho enzyme khi rau qhov kawg ntawm cov transposons thaum pab txhawb cov catalysis ntawm transposon txav mus rau lwm qhov ntawm cov genome los ntawm ntau yam txheej txheem. | Retroviral Integrases yog suav tias yog ib qho retroviral enzyme uas pab hauv kev koom ua ke ntawm cov khoom siv caj ces ntawm cov kab mob retrovirus xws li HIV rau hauv cov khoom siv caj ces (DNA) ntawm cov cell uas kis tau. |
| Sib Tw Thaj Av | |
| Cov cheeb tsam tshwj xeeb khi yog xav tau rau cov kab mob Transposases. | Tsawg lossis tsis muaj nucleotide ib ntus xav tau rau kev khi. |
Summary – Bacterial Transposases vs Retroviral Integrases
Cov kab mob kis tau raug suav hais tias yog ib qho enzyme retroviral uas pab kev sib koom ua ke ntawm cov khoom siv caj ces ntawm cov kab mob retrovirus xws li HIV rau hauv cov khoom siv caj ces (DNA) ntawm cov cell uas kis tau. Ob lub tswv yim tseem ceeb tau siv los ntawm kev hloov pauv cov noob caj noob ces rau kev hloov pauv ntawm cov genomes lossis los ntawm ib qhov chaw mus rau lwm qhov. Tsib tsev neeg ntawm transposase tau raug cais tawm tam sim no tab sis, cov tsev neeg tseem tsis tau nce ntxiv nrog cov cim tshiab transposase. Retroviral Integrase, nws yog suav tias yog ib qho enzyme retroviral uas pab hauv kev sib koom ua ke ntawm cov khoom siv caj ces ntawm cov kab mob retrovirus xws li HIV rau hauv cov khoom siv caj ces (DNA) ntawm cov cell uas kis tau. Retroviral integrase proteins feem ntau muaj peb (03) canonical domains. Lub luag haujlwm tseem ceeb ntawm retroviral integrase yog los ntxig nws cov khoom siv caj ces los tuav DNA. Cov kauj ruam no yog kauj ruam tseem ceeb tshaj plaws hauv kev kis tus kab mob HIV. Yog li ntawd, ib zaug kev koom ua ke yog tsis rov qab rau lub cell. Qhov no yog qhov txawv ntawm Bacterial transposases thiab Retroviral Integrase.
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Koj tuaj yeem rub tawm PDF version ntawm kab lus no thiab siv nws rau offline hom phiaj raws li daim ntawv ceeb toom. Thov rub tawm PDF version ntawm no: Qhov Sib txawv Ntawm Cov Kabmob Transposases thiab Retroviral Integrases
